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Q50566

- DNLI_METTH

UniProt

Q50566 - DNLI_METTH

Protein

DNA ligase

Gene

lig

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Divalent metal cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei249 – 2491ATPBy similarity
    Active sitei251 – 2511N6-AMP-lysine intermediateBy similarity
    Binding sitei256 – 2561ATPBy similarity
    Binding sitei271 – 2711ATPBy similarity
    Binding sitei301 – 3011ATPBy similarity
    Binding sitei340 – 3401ATPBy similarity
    Binding sitei417 – 4171ATPBy similarity
    Binding sitei423 – 4231ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-1582-MONOMER.
    BRENDAi6.5.1.1. 3256.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase (EC:6.5.1.1)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
    Gene namesi
    Name:lig
    Ordered Locus Names:MTH_1580
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 561561DNA ligasePRO_0000059609Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi187420.MTH1580.

    Structurei

    3D structure databases

    ProteinModelPortaliQ50566.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG1793.
    KOiK10747.
    OMAiARVQVHK.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q50566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKELLYMELA EVYHRLESTT KRLEKTEILA ELLRSVDKEL LPDVTILMLG    50
    RVFPIWSEEE LGVGIKLLMK AISLVVGVSM DEIEDEIREQ GDIGLASEKL 100
    FSRKTQTTFF SQPLTVEFVY SRLKALASAS GDRAQSKKIN ILVEVLSQAK 150
    PLEARYITRT VLEELRVGVA EGIIRDAIAR AFEVDPALVE RAHMLTNDLG 200
    MVAAVAREEG EPGLGRLNLE PGRPVKPMLA QLASSIESAI TELGRAFCET 250
    KYDGIRVQIH RCGDEISIFT RRLENITAAV PEILEGVEEA LPADDYIVEG 300
    EIIVTMDGRP ASFQYILQRV RRKYDVERLT REVPLSLFLF DVLYHREPLI 350
    DEPLRHRREV LESILSEIPG RVEASRMVDV GPDNLNDALW LFEESIREGH 400
    EGIMIKDTEA PYIPGIRGKK MLKFKAEPET LDLIVVGGTY GRGKRAHLVG 450
    SYLLAARDED SGDLVTVAHV ATGLDDETLQ QLSERMEKLA IERKGRKLLV 500
    RPEIILEVAY SEIVKSPEYE SGYSLRFPVV KRIRDDLCLD DVDTVGRIES 550
    LFQSGQPDQP G 561
    Length:561
    Mass (Da):63,089
    Last modified:November 1, 1996 - v1
    Checksum:i0E05CE1E361E5907
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51624 Genomic DNA. Translation: AAC44812.1.
    AE000666 Genomic DNA. Translation: AAB86053.1.
    PIRiH69077.
    RefSeqiNP_276692.1. NC_000916.1.
    WP_010877188.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB86053; AAB86053; MTH_1580.
    GeneIDi1471849.
    KEGGimth:MTH1580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51624 Genomic DNA. Translation: AAC44812.1 .
    AE000666 Genomic DNA. Translation: AAB86053.1 .
    PIRi H69077.
    RefSeqi NP_276692.1. NC_000916.1.
    WP_010877188.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali Q50566.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH1580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB86053 ; AAB86053 ; MTH_1580 .
    GeneIDi 1471849.
    KEGGi mth:MTH1580.

    Phylogenomic databases

    eggNOGi COG1793.
    KOi K10747.
    OMAi ARVQVHK.

    Enzyme and pathway databases

    BioCyci MTHE187420:GJNM-1582-MONOMER.
    BRENDAi 6.5.1.1. 3256.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245."
      Noelling J., Reeve J.N.
      J. Bacteriol. 179:899-908(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiDNLI_METTH
    AccessioniPrimary (citable) accession number: Q50566
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3