DNA ligase - Methanobacterium thermoautotrophicum (strain Delta H)

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Q50566 (DNLI_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MTH_1580

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 561

561

DNA ligase HAMAP MF_00407

PRO_0000059609

Sites

Active site

251

N6-AMP-lysine intermediate By similarity

Binding site

249

ATP By similarity

Binding site

256

ATP By similarity

Binding site

271

ATP By similarity

Binding site

301

ATP By similarity

Binding site

340

ATP By similarity

Binding site

417

ATP By similarity

Binding site

423

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q50566 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0E05CE1E361E5907
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FASTA

561

63,089

        10         20         30         40         50         60 
MKELLYMELA EVYHRLESTT KRLEKTEILA ELLRSVDKEL LPDVTILMLG RVFPIWSEEE 

        70         80         90        100        110        120 
LGVGIKLLMK AISLVVGVSM DEIEDEIREQ GDIGLASEKL FSRKTQTTFF SQPLTVEFVY 

       130        140        150        160        170        180 
SRLKALASAS GDRAQSKKIN ILVEVLSQAK PLEARYITRT VLEELRVGVA EGIIRDAIAR 

       190        200        210        220        230        240 
AFEVDPALVE RAHMLTNDLG MVAAVAREEG EPGLGRLNLE PGRPVKPMLA QLASSIESAI 

       250        260        270        280        290        300 
TELGRAFCET KYDGIRVQIH RCGDEISIFT RRLENITAAV PEILEGVEEA LPADDYIVEG 

       310        320        330        340        350        360 
EIIVTMDGRP ASFQYILQRV RRKYDVERLT REVPLSLFLF DVLYHREPLI DEPLRHRREV 

       370        380        390        400        410        420 
LESILSEIPG RVEASRMVDV GPDNLNDALW LFEESIREGH EGIMIKDTEA PYIPGIRGKK 

       430        440        450        460        470        480 
MLKFKAEPET LDLIVVGGTY GRGKRAHLVG SYLLAARDED SGDLVTVAHV ATGLDDETLQ 

       490        500        510        520        530        540 
QLSERMEKLA IERKGRKLLV RPEIILEVAY SEIVKSPEYE SGYSLRFPVV KRIRDDLCLD 

       550        560 
DVDTVGRIES LFQSGQPDQP G

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245." Noelling J., Reeve J.N. J. Bacteriol. 179:899-908(1997) [PubMed: 9006048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51624 Genomic DNA. Translation: AAC44812.1. AE000666 Genomic DNA. Translation: AAB86053.1.
PIR	H69077.
RefSeq	NP_276692.1. NC_000916.1.
3D structure databases
ProteinModelPortal	Q50566.
ModBase	Search...
Protein-protein interaction databases
STRING	Q50566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471849.
GenomeReviews	Gene locus MTH_1580 in contig AE000666_GR.
KEGG	mth:MTH1580.
NMPDR	fig\|187420.1.peg.1547.
Phylogenomic databases
eggNOG	arNOG04596.
HOGENOM	HBG570821.
OMA	DTERIED.
PhylomeDB	Q50566.
ProtClustDB	CLSK798208.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1580-MONOMER.
BRENDA	6.5.1.1. 3256.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METTH

Accession

Primary (citable) accession number: Q50566

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents