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Protein

DNA ligase

Gene

lig

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491ATPUniRule annotation
Active sitei251 – 2511N6-AMP-lysine intermediateUniRule annotation
Binding sitei256 – 2561ATPUniRule annotation
Binding sitei271 – 2711ATPUniRule annotation
Binding sitei301 – 3011ATPUniRule annotation
Binding sitei340 – 3401ATPUniRule annotation
Binding sitei417 – 4171ATPUniRule annotation
Binding sitei423 – 4231ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1582-MONOMER.
BRENDAi6.5.1.1. 3256.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:MTH_1580
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561DNA ligasePRO_0000059609Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1580.

Structurei

3D structure databases

ProteinModelPortaliQ50566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50566-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKELLYMELA EVYHRLESTT KRLEKTEILA ELLRSVDKEL LPDVTILMLG
60 70 80 90 100
RVFPIWSEEE LGVGIKLLMK AISLVVGVSM DEIEDEIREQ GDIGLASEKL
110 120 130 140 150
FSRKTQTTFF SQPLTVEFVY SRLKALASAS GDRAQSKKIN ILVEVLSQAK
160 170 180 190 200
PLEARYITRT VLEELRVGVA EGIIRDAIAR AFEVDPALVE RAHMLTNDLG
210 220 230 240 250
MVAAVAREEG EPGLGRLNLE PGRPVKPMLA QLASSIESAI TELGRAFCET
260 270 280 290 300
KYDGIRVQIH RCGDEISIFT RRLENITAAV PEILEGVEEA LPADDYIVEG
310 320 330 340 350
EIIVTMDGRP ASFQYILQRV RRKYDVERLT REVPLSLFLF DVLYHREPLI
360 370 380 390 400
DEPLRHRREV LESILSEIPG RVEASRMVDV GPDNLNDALW LFEESIREGH
410 420 430 440 450
EGIMIKDTEA PYIPGIRGKK MLKFKAEPET LDLIVVGGTY GRGKRAHLVG
460 470 480 490 500
SYLLAARDED SGDLVTVAHV ATGLDDETLQ QLSERMEKLA IERKGRKLLV
510 520 530 540 550
RPEIILEVAY SEIVKSPEYE SGYSLRFPVV KRIRDDLCLD DVDTVGRIES
560
LFQSGQPDQP G
Length:561
Mass (Da):63,089
Last modified:November 1, 1996 - v1
Checksum:i0E05CE1E361E5907
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51624 Genomic DNA. Translation: AAC44812.1.
AE000666 Genomic DNA. Translation: AAB86053.1.
PIRiH69077.
RefSeqiNP_276692.1. NC_000916.1.
WP_010877188.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB86053; AAB86053; MTH_1580.
GeneIDi1471849.
KEGGimth:MTH1580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51624 Genomic DNA. Translation: AAC44812.1.
AE000666 Genomic DNA. Translation: AAB86053.1.
PIRiH69077.
RefSeqiNP_276692.1. NC_000916.1.
WP_010877188.1. NC_000916.1.

3D structure databases

ProteinModelPortaliQ50566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB86053; AAB86053; MTH_1580.
GeneIDi1471849.
KEGGimth:MTH1580.

Phylogenomic databases

eggNOGiCOG1793.
KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1582-MONOMER.
BRENDAi6.5.1.1. 3256.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245."
    Noelling J., Reeve J.N.
    J. Bacteriol. 179:899-908(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiDNLI_METTH
AccessioniPrimary (citable) accession number: Q50566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.