Reviewed, UniProtKB/Swiss-Prot Q50566 (DNLI_METTH)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MTH_1580

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	DNA ligase HAMAP MF_00407		PRO_0000059609
Sites
	Active site	251	1	N6-AMP-lysine intermediate By similarity
	Binding site	249	1	ATP By similarity
	Binding site	256	1	ATP By similarity
	Binding site	271	1	ATP By similarity
	Binding site	301	1	ATP By similarity
	Binding site	340	1	ATP By similarity
	Binding site	417	1	ATP By similarity
	Binding site	423	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50566-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0E05CE1E361E5907	FASTA	561	63,089
	10 20 30 40 50 60 MKELLYMELA EVYHRLESTT KRLEKTEILA ELLRSVDKEL LPDVTILMLG RVFPIWSEEE 70 80 90 100 110 120 LGVGIKLLMK AISLVVGVSM DEIEDEIREQ GDIGLASEKL FSRKTQTTFF SQPLTVEFVY 130 140 150 160 170 180 SRLKALASAS GDRAQSKKIN ILVEVLSQAK PLEARYITRT VLEELRVGVA EGIIRDAIAR 190 200 210 220 230 240 AFEVDPALVE RAHMLTNDLG MVAAVAREEG EPGLGRLNLE PGRPVKPMLA QLASSIESAI 250 260 270 280 290 300 TELGRAFCET KYDGIRVQIH RCGDEISIFT RRLENITAAV PEILEGVEEA LPADDYIVEG 310 320 330 340 350 360 EIIVTMDGRP ASFQYILQRV RRKYDVERLT REVPLSLFLF DVLYHREPLI DEPLRHRREV 370 380 390 400 410 420 LESILSEIPG RVEASRMVDV GPDNLNDALW LFEESIREGH EGIMIKDTEA PYIPGIRGKK 430 440 450 460 470 480 MLKFKAEPET LDLIVVGGTY GRGKRAHLVG SYLLAARDED SGDLVTVAHV ATGLDDETLQ 490 500 510 520 530 540 QLSERMEKLA IERKGRKLLV RPEIILEVAY SEIVKSPEYE SGYSLRFPVV KRIRDDLCLD 550 560 DVDTVGRIES LFQSGQPDQP G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245." Noelling J., Reeve J.N. J. Bacteriol. 179:899-908(1997) [PubMed: 9006048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51624 Genomic DNA. Translation: AAC44812.1. AE000666 Genomic DNA. Translation: AAB86053.1.
PIR	H69077.
RefSeq	NP_276692.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471849.
GenomeReviews	Gene locus MTH_1580 in contig AE000666_GR.
KEGG	mth:MTH1580.
NMPDR	fig|187420.1.peg.1547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q50566.
OMA	Q50566. NIENDSE.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1580-MON.
BRENDA	6.5.1.1. 270.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METTH

Accession

Primary (citable) accession number: Q50566

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)