Reviewed, UniProtKB/Swiss-Prot Q50501 (MTD_METTH)

Names and origin

Protein names

Recommended name:
    F420-dependent methylenetetrahydromethanopterin dehydrogenase
      Short name=MTD
    EC=1.5.99.9
Alternative name(s):
    Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	MTH_1464

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity.
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (cofactor F420 route): step 1/1. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 276	275	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_0000075041

Sequences

	Sequence		Length	Mass (Da)
	Q50501-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 18445E608FEE8457	FASTA	276	29,601
	10 20 30 40 50 60 MVVKIGIIRC GNIGTSPVLD LLLDERADRP NIDVCVVGSG AKMNPDEIER AVPTMLEMDR 70 80 90 100 110 120 DFVIFISPNP GAPGPAKARE LLSEADVPAM IIGDAPGLRV KDEIEEQGLG YIIVKADPMI 130 140 150 160 170 180 GARREFLDPT EMASFNSDVI KVLAFTGAYR VVQNTIDAMI ADVEAGKAPE LPQVVIDTDV 190 200 210 220 230 240 AVEAAGYENP YAKAKAMAAY EIATKVADID VKGCFMVQDP DKYIPIVASA HEMLAAAAKL 250 260 270 AVEAREIEKA NDTVLRTPHG KEGETLSKTD LLAKPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome." Noelling J., Pihl T.D., Vriesema A., Reeve J.N. J. Bacteriol. 177:2460-2468(1995) [PubMed: 7730278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19362 Genomic DNA. Translation: AAA87412.1. AE000666 Genomic DNA. Translation: AAB85939.1.
PIR	A69062.
RefSeq	NP_276578.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471733.
GenomeReviews	Gene locus MTH_1464 in contig AE000666_GR.
KEGG	mth:MTH1464.
NMPDR	fig|187420.1.peg.1433.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q50501.
OMA	Q50501. NPYAKAK.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1464-MON.
BRENDA	1.5.99.9. 270.
Family and domain databases
HAMAP	MF_00058. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD_METTH

Accession

Primary (citable) accession number: Q50501

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)