Q504N0 (CBPA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxypeptidase A2
EC=3.4.17.15

Gene names

Name:

Cpa2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Similar to that of carboxypeptidase A (EC 3.4.17.1), but with a preference for bulkier C-terminal residues.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase M14 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallocarboxypeptidase activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 112	96	Activation peptide		PRO_0000280809
	Chain	113 – 417	305	Carboxypeptidase A2		PRO_0000280810
Regions
	Region	177 – 180	4	Substrate binding By similarity
	Region	252 – 253	2	Substrate binding By similarity
	Region	305 – 306	2	Substrate binding By similarity
Sites
	Active site	378	1	Nucleophile By similarity
	Metal binding	177	1	Zinc; catalytic By similarity
	Metal binding	180	1	Zinc; catalytic By similarity
	Metal binding	304	1	Zinc; catalytic By similarity
	Binding site	235	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	246 ↔ 269		By similarity
	Disulfide bond	318 ↔ 352		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q504N0 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: A051FF523B9E1579	FASTA	417	47,057
	10 20 30 40 50 60 MRLTPLLVAL FGYIYCQETF VGDQVLEVIP NDEEQIKTLL QLEAEEHLEL DFWKSPSVPR 70 80 90 100 110 120 QTVHVRVPFA SIQDVKVFLE SQGITYSIMI EDVQVLLDQE REEMLFNQQR ERGTNFNFGA 130 140 150 160 170 180 YHTLEEIYQE MDNLVAENPG LVSKVNIGSS FENRPMNVLK FSTGGDKPAI WLDAGIHARE 190 200 210 220 230 240 WVTQATALWT ANKIASDYGT DPAITSLLNT LDVFLLPVTN PDGYVFSQTS NRMWRKTRSK 250 260 270 280 290 300 RSGSFCVGVD PNRNWDANFG GPGASSNPCS DSYHGPSPNS EVEVKSIVDF IKSHGKVKAF 310 320 330 340 350 360 ITLHSYSQLL MFPYGYKCAK PDDFNELDEV AQRAAQSLKR LHGTSYKVGP ICSVIYQASG 370 380 390 400 410 GSIDWAYDLG IKYSFAFELR DTGYYGFLLP AKQILPTAEE TWLGLKTIME HVRDHPY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC094929 mRNA. Translation: AAH94929.1.
IPI	IPI00342962.
RefSeq	NP_001019869.1. NM_001024698.2.
UniGene	Mm.268855.
3D structure databases
HSSP	HSSP built from PDB template 1DTD based on UniProtKB P48052.
ProteinModelPortal	Q504N0.
SMR	Q504N0. Positions 18-417.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000093771.
Protein family/group databases
MEROPS	M14.002.
PTM databases
PhosphoSite	Q504N0.
Proteomic databases
PaxDb	Q504N0.
PRIDE	Q504N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000096066; ENSMUSP00000093771; ENSMUSG00000071553.
GeneID	232680.
KEGG	mmu:232680.
UCSC	uc009bfn.1. mouse.
Organism-specific databases
CTD	1358.
MGI	MGI:3617840. Cpa2.
Phylogenomic databases
eggNOG	COG2866.
GeneTree	ENSGT00560000076932.
HOGENOM	HOG000252967.
HOVERGEN	HBG050815.
InParanoid	Q504N0.
KO	K01298.
OMA	ITYSIMI.
OrthoDB	EOG4320Z5.
Gene expression databases
Bgee	Q504N0.
CleanEx	MM_CPA2.
Genevestigator	Q504N0.
Family and domain databases
Gene3D	3.30.70.340. 1 hit.
InterPro	IPR000834. Peptidase_M14. IPR003146. Prot_inh_M14A. IPR009020. Prot_inh_propept. [Graphical view]
Pfam	PF00246. Peptidase_M14. 1 hit. PF02244. Propep_M14. 1 hit. [Graphical view]
PRINTS	PR00765. CRBOXYPTASEA.
SMART	SM00631. Zn_pept. 1 hit. [Graphical view]
SUPFAM	SSF54897. Prot_inh_propept. 1 hit.
PROSITE	PS00132. CARBOXYPEPT_ZN_1. 1 hit. PS00133. CARBOXYPEPT_ZN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CPA2. mouse.
NextBio	381193.
SOURCE	Search...

Entry information

Entry name

CBPA2_MOUSE

Accession

Primary (citable) accession number: Q504N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	June 7, 2005
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program