Reviewed, UniProtKB/Swiss-Prot Q504A5 (TPMT_DANRE)

Names and origin

Protein names

Recommended name:
    Probable thiopurine S-methyltransferase
      Short name=Thiopurine methyltransferase
    EC=2.1.1.67

Gene names

Name:	tpmt
ORF Names:	zgc:109981

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine By similarity.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Probable thiopurine S-methyltransferase		PRO_0000284920
Sites
	Binding site	18	1	S-adenosyl-L-methionine By similarity
	Binding site	54	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	75	1	S-adenosyl-L-methionine By similarity
	Binding site	137	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q504A5-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 0EC5EF8310112559	FASTA	232	26,334
	10 20 30 40 50 60 MSAQADRVMD LSEWENRWQE GRTGFHRSDV HNLLKANVDK LICGRREVRF FFPLCGKAVD 70 80 90 100 110 120 MKWLADMGHT VVGVEFSEKG IKEFSQEQNL EYNEEAVADI PGAKLFKSTD GKISIYQCDL 130 140 150 160 170 180 YKFSSAVAGH FGGIWDRGAL VAINPCDRQK YASLLVSLMS SDCRYLLDTL EYNPELYKGP 190 200 210 220 230 PFFVSEDDIK TVFGGSCNID LLQSVDGFEE KHRSWGLDSL TEKLYLLTTK TQ

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC095104 mRNA. Translation: AAH95104.1.
IPI	IPI00607312.
UniGene	Dr.85410
3D structure databases
HSSP	HSSP built from PDB template 2BZG based on UniProtKB O43213.
SMR	Q504A5. Positions 3-230.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000078382; ENSDARP00000072844; ENSDARG00000055974; Danio rerio. [Genome view]
Organism-specific databases
ZFIN	ZDB-GENE-050522-141. zgc:109981.
Phylogenomic databases
eggNOG	fiNOG06887.
HOGENOM	HBG444929.
HOVERGEN	Q504A5.
InParanoid	Q504A5.
Enzyme and pathway databases
BRENDA	2.1.1.67. 96826.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_DANRE

Accession

Primary (citable) accession number: Q504A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project