ID   AHPD_MYCS2              Reviewed;         177 AA.
AC   Q50441; A0R1V8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   16-JUN-2009, entry version 35.
DE   RecName: Full=Alkyl hydroperoxide reductase ahpD;
DE            EC=1.11.1.15;
DE   AltName: Full=Alkylhydroperoxidase ahpD;
GN   Name=ahpD; OrderedLocusNames=MSMEG_4890;
OS   Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RX   MEDLINE=96256622; PubMed=8655566;
RA   Dhandayuthapani S., Zhang Y., Mudd M.H., Deretic V.;
RT   "Oxidative stress response and its role in sensitivity to isoniazid in
RT   mycobacteria: characterization and inducibility of ahpC by peroxides
RT   in Mycobacterium smegmatis and lack of expression in M. aurum and M.
RT   tuberculosis.";
RL   J. Bacteriol. 178:3641-3649(1996).
CC   -!- FUNCTION: Antioxidant protein with alkyl hydroperoxidase activity.
CC       Required for the reduction of the ahpC active site cysteine
CC       residues and for the regeneration of the ahpC enzyme activity (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
CC   -!- SUBUNIT: Homotrimer (By similarity).
CC   -!- SIMILARITY: Belongs to the ahpD family.
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DR   EMBL; CP000480; ABK74352.1; -; Genomic_DNA.
DR   EMBL; U43719; AAC44140.1; -; Genomic_DNA.
DR   RefSeq; YP_889146.1; -.
DR   GeneID; 4535695; -.
DR   GenomeReviews; CP000480_GR; MSMEG_4890.
DR   KEGG; msm:MSMEG_4890; -.
DR   TIGR; MSMEG_4890; -.
DR   OMA; Q50441; MAMNNVY.
DR   GO; GO:0008785; F:alkyl hydroperoxide reductase activity; IEA:HAMAP.
DR   GO; GO:0051920; F:peroxiredoxin activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01676; -; 1.
DR   InterPro; IPR004674; AhpD.
DR   InterPro; IPR004675; AhpD_core.
DR   InterPro; IPR003779; CMD.
DR   Pfam; PF02627; CMD; 1.
DR   TIGRFAMs; TIGR00777; ahpD; 1.
DR   TIGRFAMs; TIGR00778; ahpD_dom; 1.
PE   3: Inferred from homology;
KW   Antioxidant; Complete proteome; Disulfide bond; Oxidoreductase;
KW   Peroxidase; Redox-active center.
FT   CHAIN         1    177       Alkyl hydroperoxide reductase ahpD.
FT                                /FTId=PRO_0000064506.
FT   ACT_SITE    130    130       Proton donor (By similarity).
FT   ACT_SITE    133    133       Cysteine sulfenic acid (-SOH)
FT                                intermediate (By similarity).
FT   DISULFID    130    133       Alternate (By similarity).
FT   DISULFID    133    133       Interchain (with ahpC); in linked form
FT                                (By similarity).
FT   CONFLICT     28     30       Missing (in Ref. 2; AAC44140).
SQ   SEQUENCE   177 AA;  18837 MW;  BFDA644673FA4932 CRC64;
     MSIDNIKSAL PEYAKDLKLN LGSIANTTEL TEQQLWGALV ATAAATKNAR LLREISEDAL
     DILSEEAYNA ALGAAAIMGM NNVFYRTKGQ LDGKYDDLRA GLRMNIIGNP GVAKEDFELW
     SLAVSAINGC GHCLAAHEKT LRDADVARTT IFEAIRLASI VSGVAQALLT ADTLAAV
//