Q50441 (AHPD_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkyl hydroperoxide reductase AhpD
EC=1.11.1.15

Alternative name(s):
Alkylhydroperoxidase AhpD

Gene names

Name:	ahpD
Ordered Locus Names:	MSMEG_4890

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity By similarity. HAMAP MF_01676
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. HAMAP MF_01676
Subunit structure	Homotrimer By similarity. HAMAP MF_01676
Sequence similarities	Belongs to the AhpD family.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Alkyl hydroperoxide reductase AhpD HAMAP MF_01676		PRO_0000064506
Sites
	Active site	130	1	Proton donor By similarity
	Active site	133	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	130 ↔ 133		Alternate By similarity
	Disulfide bond	133		Interchain (with AhpC); in linked form By similarity
Experimental info
	Sequence conflict	28 – 30	3	Missing in AAC44140. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q50441 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: BFDA644673FA4932	FASTA	177	18,837
	10 20 30 40 50 60 MSIDNIKSAL PEYAKDLKLN LGSIANTTEL TEQQLWGALV ATAAATKNAR LLREISEDAL 70 80 90 100 110 120 DILSEEAYNA ALGAAAIMGM NNVFYRTKGQ LDGKYDDLRA GLRMNIIGNP GVAKEDFELW 130 140 150 160 170 SLAVSAINGC GHCLAAHEKT LRDADVARTT IFEAIRLASI VSGVAQALLT ADTLAAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Oxidative stress response and its role in sensitivity to isoniazid in mycobacteria: characterization and inducibility of ahpC by peroxides in Mycobacterium smegmatis and lack of expression in M. aurum and M. tuberculosis." Dhandayuthapani S., Zhang Y., Mudd M.H., Deretic V. J. Bacteriol. 178:3641-3649(1996) [PubMed: 8655566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74352.1. U43719 Genomic DNA. Translation: AAC44140.1.
RefSeq	YP_889146.1. NC_008596.1.
3D structure databases
ProteinModelPortal	Q50441.
SMR	Q50441. Positions 2-175.
ModBase	Search...
Protein-protein interaction databases
STRING	Q50441.
Protein family/group databases
PeroxiBase	4584. MsmAhpD.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000044705; EBMYCP00000043042; EBMYCG00000044700.
GeneID	4535695.
GenomeReviews	Gene locus MSMEG_4890 in contig CP000480_GR.
KEGG	msm:MSMEG_4890.
PATRIC	18082077. VBIMycSme59918_4772.
TIGR	MSMEG_4890.
Phylogenomic databases
eggNOG	COG2128.
GeneTree	EBGT00050000016698.
HOGENOM	HBG414634.
OMA	RAGLRMN.
ProtClustDB	CLSK791846.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4890-MONOMER.
Family and domain databases
HAMAP	MF_01676. AhpD. [Tree]
InterPro	IPR004674. AhpD. IPR004675. AhpD_core. IPR003779. CMD. [Graphical view]
KO	K04756.
Pfam	PF02627. CMD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00777. AhpD. 1 hit. TIGR00778. AhpD_dom. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AHPD_MYCS2

Accession

Primary (citable) accession number: Q50441
Secondary accession number(s): A0R1V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program