ID   DHML_METFK              Reviewed;         186 AA.
AC   Q50425; Q1H3W6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Methylamine dehydrogenase light chain;
DE            Short=MADH;
DE            EC=1.4.99.3;
DE   Flags: Precursor;
GN   Name=mauA; OrderedLocusNames=Mfla_0551;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Methylophilales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=95362696; PubMed=7635847;
RA   Gak E.R., Chistoserdov A.Y., Lidstrom M.E.;
RT   "Cloning, sequencing, and mutation of a gene for azurin in
RT   Methylobacillus flagellatum KT.";
RL   J. Bacteriol. 177:4575-4578(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N., Anderson I.,
RA   Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Methylamine dehydrogenase carries out the oxidation of
CC       methylamine. Electrons are passed from methylamine dehydrogenase
CC       to amicyanin.
CC   -!- CATALYTIC ACTIVITY: RCH(2)NH(2) + H(2)O + acceptor = RCHO + NH(3)
CC       + reduced acceptor.
CC   -!- COFACTOR: Contains 1 tryptophan tryptophylquinone per subunit (By
CC       similarity).
CC   -!- PATHWAY: One-carbon metabolism; methylamine degradation;
CC       formaldehyde from methylamine: step 1/1.
CC   -!- SUBUNIT: Heterotetramer of two light and two heavy chains.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has not been experimentally proven.
CC   -!- PTM: Tryptophan tryptophylquinone (TTQ) is formed by oxidation of
CC       the indole ring of a tryptophan to form tryptophylquinone followed
CC       by covalent cross-linking with another tryptophan residue.
CC   -!- SIMILARITY: Belongs to the aromatic amine dehydrogenase light
CC       chain family.
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DR   EMBL; L37427; AAC41474.1; -; Genomic_DNA.
DR   EMBL; CP000284; ABE48821.1; -; Genomic_DNA.
DR   RefSeq; YP_544662.1; -.
DR   HSSP; P22619; 2BBK.
DR   SMR; Q50425; 65-186.
DR   GeneID; 4000890; -.
DR   GenomeReviews; CP000284_GR; Mfla_0551.
DR   KEGG; mfa:Mfla_0551; -.
DR   NMPDR; fig|265072.7.peg.549; -.
DR   HOGENOM; Q50425; -.
DR   OMA; Q50425; LFDSWFE.
DR   BioCyc; MFLA265072:MFLA_0551-MON; -.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
DR   GO; GO:0030058; F:amine dehydrogenase activity; IEA:EC.
DR   GO; GO:0009308; P:cellular amine metabolic process; IEA:InterPro.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR016008; Amine_DH_bsu.
DR   InterPro; IPR004229; MeN_DH_Ltc.
DR   InterPro; IPR013504; MeN_DH_Ltc_C.
DR   Gene3D; G3DSA:2.60.30.10; MADH_Lt_C; 1.
DR   Pfam; PF02975; Me-amine-dh_L; 1.
DR   PIRSF; PIRSF000192; Amine_dh_beta; 1.
DR   TIGRFAMs; TIGR02659; TTQ_MADH_Lt; 1.
DR   PROSITE; PS51318; TAT; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Disulfide bond; Electron transport; Oxidoreductase;
KW   Periplasm; Signal; Transport; TTQ.
FT   SIGNAL        1     57       Tat-type signal (Potential).
FT   CHAIN        58    186       Methylamine dehydrogenase light chain.
FT                                /FTId=PRO_0000025573.
FT   MOD_RES     112    112       Tryptophylquinone (By similarity).
FT   DISULFID     78    143       By similarity.
FT   DISULFID     84    116       By similarity.
FT   DISULFID     91    176       By similarity.
FT   DISULFID     93    141       By similarity.
FT   DISULFID    101    132       By similarity.
FT   DISULFID    133    164       By similarity.
FT   CROSSLNK    112    163       Tryptophan tryptophylquinone (Trp-Trp)
FT                                (By similarity).
SQ   SEQUENCE   186 AA;  20174 MW;  E94721FDA794B523 CRC64;
     MKKNTGFDSG IEKLARKTAS KTGRRSFIGK LGGFLVGSAL LPLLPVDRRG RMNEAHAETK
     GVLGREGYKP QDKDPKSCDY WRHCSIDGNL CDCCGGSLTS CPPGTELSPS SWVASCFNPG
     DGQTYLIAYR DCCGKQTCGR CNCVNVQGEL PVYRPEFNND IVWCFGADND AMTYHCTVSP
     IVGKAS
//