Reviewed, UniProtKB/Swiss-Prot Q50425 (DHML_METFK)

Names and origin

Protein names

Recommended name:
    Methylamine dehydrogenase light chain
      Short name=MADH
    EC=1.4.99.3

Gene names

Name:	mauA
Ordered Locus Names:	Mfla_0551

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
Catalytic activity	RCH2NH2 + H2O + acceptor = RCHO + NH3 + reduced acceptor.
Cofactor	Contains 1 tryptophan tryptophylquinone per subunit By similarity.
Pathway	One-carbon metabolism; methylamine degradation; formaldehyde from methylamine: step 1/1.
Subunit structure	Heterotetramer of two light and two heavy chains.
Subcellular location	Periplasm.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Tryptophan tryptophylquinone (TTQ) is formed by oxidation of the indole ring of a tryptophan to form tryptophylquinone followed by covalent cross-linking with another tryptophan residue.
Sequence similarities	Belongs to the aromatic amine dehydrogenase light chain family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Oxidoreductase
PTM	Disulfide bond TTQ
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amine metabolic process Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	amine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 57	57	Tat-type signal Potential
	Chain	58 – 186	129	Methylamine dehydrogenase light chain		PRO_0000025573
Amino acid modifications
	Modified residue	112	1	Tryptophylquinone By similarity
	Disulfide bond	78 ↔ 143		By similarity
	Disulfide bond	84 ↔ 116		By similarity
	Disulfide bond	91 ↔ 176		By similarity
	Disulfide bond	93 ↔ 141		By similarity
	Disulfide bond	101 ↔ 132		By similarity
	Disulfide bond	133 ↔ 164		By similarity
	Cross-link	112 ↔ 163		Tryptophan tryptophylquinone (Trp-Trp) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50425-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E94721FDA794B523	FASTA	186	20,174
	10 20 30 40 50 60 MKKNTGFDSG IEKLARKTAS KTGRRSFIGK LGGFLVGSAL LPLLPVDRRG RMNEAHAETK 70 80 90 100 110 120 GVLGREGYKP QDKDPKSCDY WRHCSIDGNL CDCCGGSLTS CPPGTELSPS SWVASCFNPG 130 140 150 160 170 180 DGQTYLIAYR DCCGKQTCGR CNCVNVQGEL PVYRPEFNND IVWCFGADND AMTYHCTVSP IVGKAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT." Gak E.R., Chistoserdov A.Y., Lidstrom M.E. J. Bacteriol. 177:4575-4578(1995) [PubMed: 7635847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Methylobacillus flagellatus KT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37427 Genomic DNA. Translation: AAC41474.1. CP000284 Genomic DNA. Translation: ABE48821.1.
RefSeq	YP_544662.1.
3D structure databases
HSSP	HSSP built from PDB template 2BBK based on UniProtKB P22619.
SMR	Q50425. Positions 65-186.
ModBase	Search...
Genome annotation databases
GeneID	4000890.
GenomeReviews	Gene locus Mfla_0551 in contig CP000284_GR.
KEGG	mfa:Mfla_0551.
NMPDR	fig|265072.7.peg.549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q50425.
OMA	Q50425. LFDSWFE.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0551-MON.
Family and domain databases
InterPro	IPR016008. Amine_DH_bsu. IPR004229. MeN_DH_Ltc. IPR013504. MeN_DH_Ltc_C. [Graphical view]
Gene3D	G3DSA:2.60.30.10. MADH_Lt_C. 1 hit.
Pfam	PF02975. Me-amine-dh_L. 1 hit. [Graphical view]
PIRSF	PIRSF000192. Amine_dh_beta. 1 hit.
TIGRFAMs	TIGR02659. TTQ_MADH_Lt. 1 hit.
PROSITE	PS51318. TAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHML_METFK

Accession

Primary (citable) accession number: Q50425
Secondary accession number(s): Q1H3W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)