Reviewed, UniProtKB/Swiss-Prot Q50420 (DHMH_METFK)

Names and origin

Protein names

Recommended name:
    Methylamine dehydrogenase heavy chain
      Short name=MADH
    EC=1.4.99.3

Gene names

Name:	mauB
Ordered Locus Names:	Mfla_0548

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
Catalytic activity	RCH2NH2 + H2O + acceptor = RCHO + NH3 + reduced acceptor.
Subunit structure	Tetramer of two light and two heavy chains.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the aromatic amine dehydrogenase heavy chain family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW methylamine metabolic process Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 400	373	Methylamine dehydrogenase heavy chain		PRO_0000025578

Sequences

	Sequence		Length	Mass (Da)
	Q50420-1 [UniParc]. Last modified April 27, 2001. Version 3. Checksum: 6ACCF91ADF6979B6	FASTA	400	44,330
	10 20 30 40 50 60 MTTFQPGRLA GQLAATALLA ATCSAFAADA PTTVPSSLGA IGTTPTLDNI TMEPGLPSDA 70 80 90 100 110 120 KRVYVLDPGH FHVTTTVYTI DGNKNNLLGM TDTGKLANVM LSSDGKFFVT SNTTYSRIAR 130 140 150 160 170 180 GKRDDYVEVI DAQSHKVLAD IDIPEGRFLT GVMNRMASLS TDNKYMLFQQ FAPSPAVGLV 190 200 210 220 230 240 DLEKKSFVKM MDIPDCYQIF PVPNQSFYMH CRDGSLQQFG YDDKGNLKPM KPTKVFHGED 250 260 270 280 290 300 DYLFVNPYYS NGSGRLVWPT YEGRIFQAKL TDKKVDFMKP FELFTEAEKK ANWRPGGWQV 310 320 330 340 350 360 VAYHKARNEI YVLADQRAKW THVTASRYVF VVDGTTGKRL RRIDLGHEID GISVTQDANP 370 380 390 400 NLYAVSAEAK TLFTFNAVTG KETGKVDELG RAPTISLTMD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of methylamine utilization genes (mau) in 'Methylobacillus flagellatum ' KT and analysis of mau mutants." Gak E.R., Tsygankov Y.D., Chistoserdov A.Y. Microbiology 143:1827-1835(1997) [PubMed: 9202457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Methylobacillus flagellatus KT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT." Gak E.R., Chistoserdov A.Y., Lidstrom M.E. J. Bacteriol. 177:4575-4578(1995) [PubMed: 7635847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-68 AND 332-400.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF188997 Genomic DNA. Translation: AAC41469.2. CP000284 Genomic DNA. Translation: ABE48818.1.
RefSeq	YP_544659.1.
3D structure databases
HSSP	HSSP built from PDB template 2MTA based on UniProtKB P29894.
ModBase	Search...
Protein-protein interaction databases
STRING	Q50420.
Genome annotation databases
GeneID	4000887.
GenomeReviews	Gene locus Mfla_0548 in contig CP000284_GR.
KEGG	mfa:Mfla_0548.
NMPDR	fig|265072.7.peg.546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q50420.
OMA	RWDLAQQ.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0548-MON.
Family and domain databases
InterPro	IPR013476. MeN_DH_hvc. IPR009451. Metamine_DH_Hsu. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF06433. Me-amine-dh_H. 1 hit. [Graphical view]
PIRSF	PIRSF017797. TTQ_MADH_Hv. 1 hit.
TIGRFAMs	TIGR02658. TTQ_MADH_Hv. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHMH_METFK

Accession

Primary (citable) accession number: Q50420
Secondary accession number(s): Q1H3W9, Q50413

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)