Q50394 (EMBA_MYCSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable arabinosyltransferase A
EC=2.4.2.-

Gene names

Name:

embA

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	1092 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Miscellaneous	This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1092	1092	Probable arabinosyltransferase A		PRO_0000220563
Regions
	Transmembrane	21 – 43	23	Helical; Potential
	Transmembrane	214 – 233	20	Helical; Potential
	Transmembrane	249 – 271	23	Helical; Potential
	Transmembrane	324 – 346	23	Helical; Potential
	Transmembrane	353 – 372	20	Helical; Potential
	Transmembrane	382 – 399	18	Helical; Potential
	Transmembrane	404 – 426	23	Helical; Potential
	Transmembrane	517 – 534	18	Helical; Potential
	Transmembrane	541 – 563	23	Helical; Potential
	Transmembrane	568 – 590	23	Helical; Potential
	Transmembrane	602 – 624	23	Helical; Potential
	Transmembrane	639 – 661	23	Helical; Potential
	Transmembrane	682 – 704	23	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q50394 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: 62399B9F9EB24F3A	FASTA	1,092	117,055
	10 20 30 40 50 60 MPGDEQRERT ADDAVTEPSR IARLIAVVAG IAGVLLCGLV PLLPVEETTA TVLWPQGVGA 70 80 90 100 110 120 DGNVTELTAP LVAGAPRALD VTIPCRAVAE LPADGGVVFS TNPAGGIEAG RNGMFIRANA 130 140 150 160 170 180 DVVYVAFRDT VAAVAPREAV DSGACSEIHV WADVSAVGAD FAGIPDASGT LPVDKRPQVS 190 200 210 220 230 240 GVFTDLKVPA QPGLAARIDI DTRFITSPTL LKTAVMVLGL ACVIGSIVAL ALLDRGWRRR 250 260 270 280 290 300 PARTRGRAGL WTWITDTGVI GGLLIWHIVG APTSDDGYNM TIARVASEAG YTTNYYRYFG 310 320 330 340 350 360 ASEAPFDWYQ SVLSHLASIS TAGVWMRLPA TAAAIATWLI ISRCVLPRIG RRVAANRVAM 370 380 390 400 410 420 LTAGATFLAA WLPFNNGLRP EPLIAFAVIT VWMLVENSIG TRRLWPAAVA IVIAMFSVTL 430 440 450 460 470 480 APQGLIALAP LLVGARAIGR VVTARRAAPG SWRPCPLAAS VAVVFVIIFR DQTLATVAES 490 500 510 520 530 540 VRIKYVVGPT IPWYQEFLRY YFLTVEDSVD GSLTRRFAVL VLLLCLFGLI MVLLRRGRVP 550 560 570 580 590 600 GAVSGPLWRL CGSTAIGLLL LILTPTKWAI QFGAFAGLAG ALGGVTAFAF ARVGLHSRRN 610 620 630 640 650 660 LALYVTALLF ILAWATSGLN GWFYVGNYGV PWFDKQPVIA HYPVTTIFLV LAIVGGLLAG 670 680 690 700 710 720 WLHFRMDYAG HTEVADTGRN RALASTPLLI VATIMVVLEL GSMVKATVGR YPVYTVGSAN 730 740 750 760 770 780 IAALRSAGDS CAMADAVLVE ADPNEGMLQP VPGQRFGDYG PLGGEDPVGF TPSGVSEHLE 790 800 810 820 830 840 PEPVGTNPGT PNSEGPVDKP NIGIAYAGDT GGGYAPEGVN GSRVFLPFGL DPSRTPVMGS 850 860 870 880 890 900 YGENKLAAKA TSAWYQLPPR TPDRPLVTVA AAGAIWYYEE DGSFNYGQSL KLQWGVHRPD 910 920 930 940 950 960 GTYQALSEVQ PIDIFQQKAW RNLRFPLAWA PPEANVARIV ADDPNLSEDQ WCAFTPPRVP 970 980 990 1000 1010 1020 VLQTAQQFLG SQTPVLMDIA TAANFPCQRP FAERLGVAEL PEYRIIPNFK QMVVSSNQWQ 1030 1040 1050 1060 1070 1080 SAADGGPFLF IQALLRTEAI PTYLRDDWYR DWGSIERYIR VVPQEQAPTA AIEEGSTRVF 1090 GWSRGGPIRA LP

References

[1]

"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: imm30.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46844 Genomic DNA. Translation: AAC45272.1.
PIR	T45095.
3D structure databases
ProteinModelPortal	Q50394.
ModBase	Search...
Protein family/group databases
CAZy	GT53. Glycosyltransferase Family 53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007680. Mycobac_arabinan_synth. [Graphical view]
Pfam	PF04602. Arabinose_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EMBA_MYCSM

Accession

Primary (citable) accession number: Q50394

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program