Q50313 (DPO3B_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta
EC=2.7.7.7

Gene names

Name:	dnaN
Ordered Locus Names:	MPN_001
ORF Names:	MP153

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	DNA polymerase III subunit beta		PRO_0000105446

Sequences

	Sequence		Length	Mass (Da)
	Q50313 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AE3F5717962D3DFB	FASTA	380	43,856
	10 20 30 40 50 60 MKVLINKNEL NKILKKLNNV IVSNNKMKPY HSYLLIEATE KEINFYANNE YFSAKCTLAE 70 80 90 100 110 120 NIDVLEEGEV IVKGKIFSEL INGIKEDIIT IQEKDQTLLV KTKKTNINLN TIDKKEFPRI 130 140 150 160 170 180 RFNQNVDLKE FDELKIQHSL LTKGLKKIAH AVSTFRESTR KFNGVNFNGS NGKQIFLEAS 190 200 210 220 230 240 DSYKLSVYEI KQKTDPFNFI VETNLLSFIN SFNPEGGDLI SIFFRKEHKD DLSTELLIKL 250 260 270 280 290 300 DNFLINYTSI NESFPRVMQL FDFEPETKVT IQKNELKDAL QRILTLAQNE RFFLCDMQVT 310 320 330 340 350 360 NSHLKINSNV QNIGASLEEV TCLKFEGHKL NIAVNALSLL EHIDSFDTDE IELYFQGSNK 370 380 YFLISSNNEP ELKEILVPSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes." Hilbert H., Himmelreich R., Plagens H., Herrmann R. Nucleic Acids Res. 24:628-639(1996) [PubMed: 8604303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34816 Genomic DNA. Translation: AAC43645.1. U00089 Genomic DNA. Translation: AAG34739.1.
PIR	S62836.
RefSeq	NP_109689.1. NC_000912.1.
3D structure databases
ProteinModelPortal	Q50313.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q50313. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877175.
GenomeReviews	Gene locus MPN_001 in contig U00089_GR.
KEGG	mpn:MPN001.
PATRIC	20021275. VBIMycPne110_0001.
Phylogenomic databases
HOGENOM	HBG129976.
OMA	NADANFE.
PhylomeDB	Q50313.
ProtClustDB	CLSK437066.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN001-MONOMER.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR022637. DNA_polIII_beta_cen. IPR024693. DNA_polIII_beta_dom. IPR022634. DNA_polIII_beta_N. [Graphical view]
Gene3D	G3DSA:3.10.150.10. DNA_polIII_beta. 3 hits.
KO	K02338.
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. Dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_MYCPN

Accession

Primary (citable) accession number: Q50313

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program