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Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

samhd1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur (By similarity).By similarity

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95dGTP 1By similarity1
Binding sitei98dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei128SubstrateBy similarity1
Binding sitei143SubstrateBy similarity1
Metal bindingi146Zinc; via tele nitrogenBy similarity1
Metal bindingi185Zinc; via tele nitrogenBy similarity1
Metal bindingi186ZincBy similarity1
Binding sitei189SubstrateBy similarity1
Active sitei212By similarity1
Metal bindingi291ZincBy similarity1
Binding sitei295SubstrateBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei313dGTP 2By similarity1
Binding sitei338dGTP 2By similarity1
Binding sitei346SubstrateBy similarity1
Binding sitei356dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei357dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei431dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei435dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei502dGTP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 124dGTPBy similarity9
Nucleotide bindingi116 – 124dGTP 1By similarity9
Nucleotide bindingi332 – 334dGTP 2By similarity3

GO - Molecular functioni

GO - Biological processi

  • dATP catabolic process Source: UniProtKB
  • defense response to virus Source: UniProtKB
  • dGTP catabolic process Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • regulation of innate immune response Source: ZFIN

Keywordsi

Molecular functionHydrolase
Biological processAntiviral defense, Immunity, Innate immunity
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:samhd1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-090313-386. samhd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003619701 – 622Deoxynucleoside triphosphate triphosphohydrolase SAMHD1Add BLAST622

Proteomic databases

PaxDbiQ502K2.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form (By similarity).By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096087.

Structurei

3D structure databases

ProteinModelPortaliQ502K2.
SMRiQ502K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 89SAMPROSITE-ProRule annotationAdd BLAST64
Domaini143 – 296HDPROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 295Substrate bindingBy similarity7
Regioni350 – 355Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the SAMHD1 family.Curated

Phylogenomic databases

eggNOGiKOG2681. Eukaryota.
COG1078. LUCA.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ502K2.
PhylomeDBiQ502K2.

Family and domain databases

CDDicd00077. HDc. 1 hit.
InterProiView protein in InterPro
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
PfamiView protein in Pfam
PF01966. HD. 1 hit.
PF07647. SAM_2. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
SM00454. SAM. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiView protein in PROSITE
PS51831. HD. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q502K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENRIKRPRV DGECVKSINN GEFDRWDVED TVAYLRGEGL QEWAQIFKDN
60 70 80 90 100
GITGAGLLCL TEAHLEKMGV SPLGARLHIL HCLQKLSQIS TEPMKVFNDP
110 120 130 140 150
IHGHIELHPL LLHFIDTPQF QRLRHIKQLG GTYLVFPGAS HNRFEHSIGV
160 170 180 190 200
GYLAGCLVKA LNERQPELFI TKQDILCVQI AGLCHDLGHG PFSHMFDGMF
210 220 230 240 250
IPKARPADKW KHEIASVQMF DHLVEVNGLE AVMLHHGMRL PEDLIFIKEQ
260 270 280 290 300
IAGPLESAVL DQSQWPYKGR PVEKSFLYEV VANKRNGIDV DKWDYFARDC
310 320 330 340 350
YHLGIQNNFD YQRFLRFARV CEVKGRKHIC TRDKEVGNLY DMFHTRNCLH
360 370 380 390 400
RRAYQHKVGN IIETMITEAF LKADPHIQIQ GSSGRIFTIS SAIEDMEAYS
410 420 430 440 450
KLTDHIFEQI LYSSGPELSE ARAILQNIIC RRLYKCVGQT TSETNVDVSQ
460 470 480 490 500
EKLLDWAKEL ARSKPTGTEG NLIAEDFVVS VIHMDYGMKE KNPINNVHFY
510 520 530 540 550
CKNDPTKAIK IHKKQVSKLL PERFAEQLIR VYCKKTDDKS LEAAKKYFVQ
560 570 580 590 600
WCMDRNFTKP QDGDIIAPEL TPLKQDWHAR EDEDEEEEEK HRQNQTLPHH
610 620
TPQRTGRNVK VDLFQARGET KL
Length:622
Mass (Da):71,668
Last modified:February 10, 2009 - v2
Checksum:iC4DA54301CD2C77D
GO

Sequence cautioni

The sequence AAH95665 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC095665 mRNA. Translation: AAH95665.1. Different initiation.
UniGeneiDr.79209.

Similar proteinsi

Entry informationi

Entry nameiSAMH1_DANRE
AccessioniPrimary (citable) accession number: Q502K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 10, 2009
Last modified: September 27, 2017
This is version 70 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families