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Q502K2 (SAMH1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Short name=dNTPase
EC=3.1.5.-
Gene names
Name:samhd1
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur By similarity.

Catalytic activity

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.

Subunit structure

Homodimer. Homotetramer; in dGTP-bound form By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the SAMHD1 family.

Contains 1 HD domain.

Contains 1 SAM (sterile alpha motif) domain.

Sequence caution

The sequence AAH95665.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
PRO_0000361970

Regions

Domain26 – 8964SAM
Domain143 – 299157HD
Nucleotide binding116 – 1249dGTP By similarity
Nucleotide binding116 – 1249dGTP 1 By similarity
Nucleotide binding332 – 3343dGTP 2 By similarity
Region289 – 2957Substrate binding By similarity
Region350 – 3556Substrate binding By similarity

Sites

Active site2121 By similarity
Metal binding1461Zinc; via tele nitrogen By similarity
Metal binding1851Zinc; via tele nitrogen By similarity
Metal binding1861Zinc By similarity
Metal binding2911Zinc By similarity
Binding site951dGTP 1 By similarity
Binding site981dGTP 2; via amide nitrogen; shared with neighboring subunit By similarity
Binding site1281Substrate By similarity
Binding site1431Substrate By similarity
Binding site1891Substrate By similarity
Binding site2951Substrate By similarity
Binding site2991Substrate By similarity
Binding site3131dGTP 2 By similarity
Binding site3381dGTP 2 By similarity
Binding site3461Substrate By similarity
Binding site3561dGTP 2; shared with neighboring subunit By similarity
Binding site3571dGTP 2; shared with neighboring subunit By similarity
Binding site4311dGTP 1; shared with neighboring subunit By similarity
Binding site4351dGTP 1; shared with neighboring subunit By similarity
Binding site5021dGTP 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q502K2 [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: C4DA54301CD2C77D

FASTA62271,668
        10         20         30         40         50         60 
MENRIKRPRV DGECVKSINN GEFDRWDVED TVAYLRGEGL QEWAQIFKDN GITGAGLLCL 

        70         80         90        100        110        120 
TEAHLEKMGV SPLGARLHIL HCLQKLSQIS TEPMKVFNDP IHGHIELHPL LLHFIDTPQF 

       130        140        150        160        170        180 
QRLRHIKQLG GTYLVFPGAS HNRFEHSIGV GYLAGCLVKA LNERQPELFI TKQDILCVQI 

       190        200        210        220        230        240 
AGLCHDLGHG PFSHMFDGMF IPKARPADKW KHEIASVQMF DHLVEVNGLE AVMLHHGMRL 

       250        260        270        280        290        300 
PEDLIFIKEQ IAGPLESAVL DQSQWPYKGR PVEKSFLYEV VANKRNGIDV DKWDYFARDC 

       310        320        330        340        350        360 
YHLGIQNNFD YQRFLRFARV CEVKGRKHIC TRDKEVGNLY DMFHTRNCLH RRAYQHKVGN 

       370        380        390        400        410        420 
IIETMITEAF LKADPHIQIQ GSSGRIFTIS SAIEDMEAYS KLTDHIFEQI LYSSGPELSE 

       430        440        450        460        470        480 
ARAILQNIIC RRLYKCVGQT TSETNVDVSQ EKLLDWAKEL ARSKPTGTEG NLIAEDFVVS 

       490        500        510        520        530        540 
VIHMDYGMKE KNPINNVHFY CKNDPTKAIK IHKKQVSKLL PERFAEQLIR VYCKKTDDKS 

       550        560        570        580        590        600 
LEAAKKYFVQ WCMDRNFTKP QDGDIIAPEL TPLKQDWHAR EDEDEEEEEK HRQNQTLPHH 

       610        620 
TPQRTGRNVK VDLFQARGET KL 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC095665 mRNA. Translation: AAH95665.1. Different initiation.
UniGeneDr.79209.

3D structure databases

ProteinModelPortalQ502K2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000096087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ZFINZDB-GENE-090313-386. samhd1.

Phylogenomic databases

eggNOGCOG1078.
HOGENOMHOG000264286.
HOVERGENHBG054208.
InParanoidQ502K2.
PhylomeDBQ502K2.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
1.10.3210.10. 2 hits.
InterProIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
[Graphical view]
PfamPF01966. HD. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSAMH1_DANRE
AccessionPrimary (citable) accession number: Q502K2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families