Reviewed, UniProtKB/Swiss-Prot Q502J0 (PCAT2_DANRE)

Names and origin

Protein names

Recommended name:
    Lysophosphatidylcholine acyltransferase 2
      Short name=LysoPC acyltransferase 2
      Short name=LPC acyltransferase 2
      Short name=LPCAT-2
    EC=2.3.1.-
Alternative name(s):
    1-alkylglycerophosphocholine O-acetyltransferase
    EC=2.3.1.67
    Acetyl-CoA:lyso-platelet-activating factor acetyltransferase
      Short name=Acetyl-CoA:lyso-PAF acetyltransferase
      Short name=Lyso-PAF acetyltransferase
      Short name=LysoPAFAT
    1-acylglycerophosphocholine O-acyltransferase
    EC=2.3.1.23
    Acyltransferase-like 1

Gene names

Name:	lpcat2
Synonyms:	aytl1
ORF Names:	zgc:112165

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine. Acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine = CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine.
Pathway	Lipid metabolism; phospholipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. Contains 3 EF-hand domains.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Repeat Signal-anchor Transmembrane
Ligand	Calcium
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerophosphocholine O-acyltransferase activity Inferred from electronic annotation. Source: EC 1-alkylglycerophosphocholine O-acetyltransferase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Lysophosphatidylcholine acyltransferase 2		PRO_0000247063
Regions
	Topological domain	1 – 50	50	Cytoplasmic Potential
	Transmembrane	51 – 71	21	Signal-anchor for type II membrane protein Potential
	Topological domain	72 – 529	458	Lumenal Potential
	Domain	373 – 408	36	EF-hand 1
	Domain	410 – 445	36	EF-hand 2
	Domain	445 – 480	36	EF-hand 3
	Calcium binding	386 – 397	12	1 Potential
	Calcium binding	423 – 434	12	2 Potential
	Calcium binding	458 – 469	12	3 Potential
	Motif	128 – 133	6	HXXXXD motif
Amino acid modifications
	Glycosylation	207	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q502J0-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: F54CA365FE6461D1	FASTA	529	59,424
	10 20 30 40 50 60 MPPPHRVFAL PRQQSLLLPA VINPFVHDLS LSTADITKCF LLGIILVPLR AIFLLLVLLV 70 80 90 100 110 120 MWPVSVIITF GQSLKGVVEP MTGWRRFLHR RVMTFLGRMY FFGMGFKVVV KGKKASTLEA 130 140 150 160 170 180 PILAVAPHSS FFDAIACIES GLPSTVSRIE SLEAPIFGRF LRCVQPVLVS RTDPDSRRNT 190 200 210 220 230 240 IIEIERRAKS GGHWPQVLIF PEGTCTNRSC LITFKQGGFV PGVPVQPVLI RYPNKLDTVT 250 260 270 280 290 300 WTWQGPKSAR LLLLTLCQLC TTVEVEFLPP QVPTEMEKKC PLKFAQSVRA VMAKSLKLPV 310 320 330 340 350 360 TDHTYEDCRL MIAAGELTLP MEAGLVEFTK ISRKLELKWD NVKKELESFA NIACSCKGGR 370 380 390 400 410 420 ITVEEFASFL KLPISPALQQ LFALFDRNGD GTIDFREYVI GVTVLCRPAN NEEVIQTAFK 430 440 450 460 470 480 LFDIDEDNCI TQEEFSSLLR SALGVCDLDV HSLFREIDAD GSGHITYDEF RSFALNHPEY 490 500 510 520 AKLFTTYIEL QRYQGLQGDE TDFDSAFSHC CTAAYDEYQE DSASDKKDD

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Larva.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC095679 mRNA. Translation: AAH95679.1.
IPI	IPI00607431.
RefSeq	NP_001018492.1.
UniGene	Dr.14414
3D structure databases
SMR	Q502J0. Positions 304-475, 347-493.
ModBase	Search...
Protein-protein interaction databases
STRING	Q502J0.
Genome annotation databases
Ensembl	ENSDART00000074963; ENSDARP00000069449; ENSDARG00000053010; Danio rerio. [Genome view]
GeneID	553683.
KEGG	dre:553683.
Organism-specific databases
ZFIN	ZDB-GENE-050522-229. zgc:112165.
Phylogenomic databases
eggNOG	fiNOG05133.
HOGENOM	HBG446767.
HOVERGEN	Q502J0.
InParanoid	Q502J0.
PhylomeDB	Q502J0.
Gene expression databases
ArrayExpress	Q502J0.
Bgee	Q502J0.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCAT2_DANRE

Accession

Primary (citable) accession number: Q502J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project