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Protein

Lysophosphatidylcholine acyltransferase 2

Gene

lpcat2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. May catalyze the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine. May also cataylze the conversion of as well 1-O-alkyl-sn-glycero-3-phosphocholine to 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine and 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.
Acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine = CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine.

Pathwayi: phospholipid metabolism

This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi386 – 397121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi423 – 434122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi458 – 469123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00085.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophosphatidylcholine acyltransferase 2 (EC:2.3.1.23)
Short name:
LPC acyltransferase 2
Short name:
LPCAT-2
Short name:
LysoPC acyltransferase 2
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 11 (EC:2.3.1.51)
Short name:
1-AGP acyltransferase 11
Short name:
1-AGPAT 11
1-acylglycerophosphocholine O-acyltransferase
1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67)
Acyltransferase-like 1
Gene namesi
Name:lpcat2
Synonyms:aytl1
ORF Names:zgc:112165
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050522-229. lpcat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5050CytoplasmicSequence analysisAdd
BLAST
Transmembranei51 – 7121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini72 – 529458LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Lysophosphatidylcholine acyltransferase 2PRO_0000247063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ502J0.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000111397.

Structurei

3D structure databases

ProteinModelPortaliQ502J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini373 – 40836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini410 – 44536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini445 – 48036EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi128 – 1336HXXXXD motif
Motifi202 – 2054EGTC motif

Domaini

The HXXXXD motif is essential for acyltransferase activity.By similarity

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4666. Eukaryota.
ENOG410XSIQ. LUCA.
HOGENOMiHOG000234374.
HOVERGENiHBG060273.
InParanoidiQ502J0.
KOiK13510.
PhylomeDBiQ502J0.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00563. PlsC. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q502J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPPHRVFAL PRQQSLLLPA VINPFVHDLS LSTADITKCF LLGIILVPLR
60 70 80 90 100
AIFLLLVLLV MWPVSVIITF GQSLKGVVEP MTGWRRFLHR RVMTFLGRMY
110 120 130 140 150
FFGMGFKVVV KGKKASTLEA PILAVAPHSS FFDAIACIES GLPSTVSRIE
160 170 180 190 200
SLEAPIFGRF LRCVQPVLVS RTDPDSRRNT IIEIERRAKS GGHWPQVLIF
210 220 230 240 250
PEGTCTNRSC LITFKQGGFV PGVPVQPVLI RYPNKLDTVT WTWQGPKSAR
260 270 280 290 300
LLLLTLCQLC TTVEVEFLPP QVPTEMEKKC PLKFAQSVRA VMAKSLKLPV
310 320 330 340 350
TDHTYEDCRL MIAAGELTLP MEAGLVEFTK ISRKLELKWD NVKKELESFA
360 370 380 390 400
NIACSCKGGR ITVEEFASFL KLPISPALQQ LFALFDRNGD GTIDFREYVI
410 420 430 440 450
GVTVLCRPAN NEEVIQTAFK LFDIDEDNCI TQEEFSSLLR SALGVCDLDV
460 470 480 490 500
HSLFREIDAD GSGHITYDEF RSFALNHPEY AKLFTTYIEL QRYQGLQGDE
510 520
TDFDSAFSHC CTAAYDEYQE DSASDKKDD
Length:529
Mass (Da):59,424
Last modified:June 7, 2005 - v1
Checksum:iF54CA365FE6461D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC095679 mRNA. Translation: AAH95679.1.
RefSeqiNP_001018492.1. NM_001020656.1.
UniGeneiDr.14414.

Genome annotation databases

GeneIDi553683.
KEGGidre:553683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC095679 mRNA. Translation: AAH95679.1.
RefSeqiNP_001018492.1. NM_001020656.1.
UniGeneiDr.14414.

3D structure databases

ProteinModelPortaliQ502J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000111397.

Proteomic databases

PaxDbiQ502J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi553683.
KEGGidre:553683.

Organism-specific databases

CTDi54947.
ZFINiZDB-GENE-050522-229. lpcat2.

Phylogenomic databases

eggNOGiKOG4666. Eukaryota.
ENOG410XSIQ. LUCA.
HOGENOMiHOG000234374.
HOVERGENiHBG060273.
InParanoidiQ502J0.
KOiK13510.
PhylomeDBiQ502J0.

Enzyme and pathway databases

UniPathwayiUPA00085.

Miscellaneous databases

NextBioi20880414.
PROiQ502J0.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00563. PlsC. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Larva.

Entry informationi

Entry nameiPCAT2_DANRE
AccessioniPrimary (citable) accession number: Q502J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 7, 2005
Last modified: December 9, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.