Reviewed, UniProtKB/Swiss-Prot Q502I6 (NB5R4_DANRE)

Names and origin

Protein names

Recommended name:
    Cytochrome b5 reductase 4
    EC=1.6.2.2
Alternative name(s):
    Flavohemoprotein b5/b5R
      Short name=b5+b5R
    cb5/cb5R

Gene names

Name:	cyb5r4
ORF Names:	zgc:112177

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductase involved in endolasmic reticulum stress response pathway By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Endoplasmic reticulum. Note: Soluble protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 CS domain. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Ligand	FAD Flavoprotein Heme Iron Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Cytochrome b5 reductase 4		PRO_0000287559
Regions
	Domain	56 – 132	77	Cytochrome b5 heme-binding
	Domain	173 – 264	92	CS
	Domain	281 – 392	112	FAD-binding FR-type
	Nucleotide binding	372 – 387	16	FAD By similarity
	Nucleotide binding	399 – 431	33	FAD By similarity
Sites
	Metal binding	91	1	Iron (heme axial ligand) By similarity
	Metal binding	114	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q502I6-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 0A58916CDCB39FD0	FASTA	527	59,452
	10 20 30 40 50 60 MLNVPSQAFP AAGSQQRVAP AGQSRNKVVL KPGHSLLDWI RLTKSGQDLT GLRGRLIEVT 70 80 90 100 110 120 EDELKKHNTK KDCWTCIRGM VYNLSAYMDF HPGGEEELMR AAGIDSTDLF DEVHRWVNYE 130 140 150 160 170 180 SMLKECLVGR MAVKPSPALQ AHTEKTESTH LNGLSAPPSL RPEPLSAPLP AKDHRPRYDW 190 200 210 220 230 240 FQTDGTVNIV VYTKRKIPSA GCAVVDLQDD NLRVEMLLGR MSYLLYWRLS SRVQDHVDVQ 250 260 270 280 290 300 TAHSVGKVQL CLRKSVKEKW TQLGQSLEHH DTFIQCKDRG LFYRECVLLS KTDVTHNTQL 310 320 330 340 350 360 LRLQLPRGSR MQVPVGRHVY LKTSVQGTDV VKPYTAVDQM LIPPSQSSAE VGSDIHLMIK 370 380 390 400 410 420 VYPDGVLTPH IANLPIGASL SVGGPEGSFT LRVLRDVTHL YMLAAGTGFT PMARLIRLAL 430 440 450 460 470 480 QDFTVIRKMK LMFFNRQERD ILWQSQLDEL CTKEERFEVQ HVLSEPADSW TGRRGRIDAC 490 500 510 520 MLQNFLERPE NSKCLVCVCG PAGFTESAVQ LVRQLDFSEE ELHVFQA

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC095683 mRNA. Translation: AAH95683.1.
IPI	IPI00627513.
RefSeq	NP_001018496.1.
UniGene	Dr.85123
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000020898. Danio rerio. [Contig view]
GeneID	553685.
KEGG	dre:553685.
Organism-specific databases
ZFIN	ZDB-GENE-050522-225. zgc:112177.
Phylogenomic databases
HOGENOM	Q502I6.
HOVERGEN	Q502I6.
Enzyme and pathway databases
BRENDA	1.6.2.2. 96826.
Gene expression databases
ArrayExpress	Q502I6.
Bgee	Q502I6.
Family and domain databases
InterPro	IPR017447. CS. IPR007052. CS_domain. IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF04969. CS. 1 hit. PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51203. CS. 1 hit. PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R4_DANRE

Accession

Primary (citable) accession number: Q502I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project