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Protein

Adenylate kinase

Gene

adk

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Pathway: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from ADP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenylate kinase (adk)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from ADP, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351AMPUniRule annotation
Binding sitei40 – 401AMPUniRule annotation
Binding sitei98 – 981AMPUniRule annotation
Binding sitei129 – 1291ATPUniRule annotation
Metal bindingi132 – 1321Zinc; structuralUniRule annotation
Metal bindingi135 – 1351Zinc; structuralUniRule annotation
Metal bindingi152 – 1521Zinc; structuralUniRule annotation
Metal bindingi155 – 1551Zinc; structuralUniRule annotation
Binding sitei162 – 1621AMPUniRule annotation
Binding sitei173 – 1731AMPUniRule annotation
Binding sitei211 – 2111ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196ATPUniRule annotation
Nucleotide bindingi61 – 633AMPUniRule annotation
Nucleotide bindingi91 – 944AMPUniRule annotation
Nucleotide bindingi138 – 1392ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-192-MONOMER.
UniPathwayiUPA00588; UER00649.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinaseUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AKUniRule annotation
Alternative name(s):
ATP-AMP transphosphorylaseUniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Gene namesi
Name:adkUniRule annotation
Ordered Locus Names:MPN_185
ORF Names:MP646
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Adenylate kinasePRO_0000158804Add
BLAST

Proteomic databases

PaxDbiQ50299.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ50299. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ50299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 6330NMPbindUniRule annotationAdd
BLAST
Regioni128 – 16538LIDUniRule annotationAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0563.
KOiK00939.
OMAiIDEITCK.
OrthoDBiEOG679TH4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMENKFLFL GAPGVGKGTL AKQVANTTGL FHLSTGDIFR SVMQEQGALS
60 70 80 90 100
QTLAHYMNQG LYVPDELTNQ TFWHFVTTHQ NELHKGFILD GYPRTLNQLE
110 120 130 140 150
FLQSKLQLDQ VFHLKLSDPQ VLVARILNRL VCPSCGSVYN KQSKPPLKAN
160 170 180 190 200
QCDRCHATLQ ARNDDTEAVI LKRLTLYEDT VKPLIEAFTK QGILTVIEAQ
210
LPLEQQVNLV QQLVH
Length:215
Mass (Da):24,255
Last modified:November 1, 1997 - v1
Checksum:i3F4258BEFA24E8E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34795 Genomic DNA. Translation: AAC43696.1.
U00089 Genomic DNA. Translation: AAB96294.1.
PIRiS62823.
RefSeqiNP_109873.1. NC_000912.1.
WP_010874542.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96294; AAB96294; MPN_185.
GeneIDi876756.
KEGGimpn:MPN185.
PATRICi20021693. VBIMycPne110_0203.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34795 Genomic DNA. Translation: AAC43696.1.
U00089 Genomic DNA. Translation: AAB96294.1.
PIRiS62823.
RefSeqiNP_109873.1. NC_000912.1.
WP_010874542.1. NC_000912.1.

3D structure databases

ProteinModelPortaliQ50299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ50299. 1 interaction.

Proteomic databases

PaxDbiQ50299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96294; AAB96294; MPN_185.
GeneIDi876756.
KEGGimpn:MPN185.
PATRICi20021693. VBIMycPne110_0203.

Phylogenomic databases

eggNOGiCOG0563.
KOiK00939.
OMAiIDEITCK.
OrthoDBiEOG679TH4.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00649.
BioCyciMPNE272634:GJ6Z-192-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes."
    Hilbert H., Himmelreich R., Plagens H., Herrmann R.
    Nucleic Acids Res. 24:628-639(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29342 / M129.
  2. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiKAD_MYCPN
AccessioniPrimary (citable) accession number: Q50299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.