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Q50291 (TRUA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Synonyms:hisT
Ordered Locus Names:MPN_196
ORF Names:MP635
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_0000057413

Sites

Active site521Nucleophile By similarity
Binding site1101Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q50291 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A5DE0B36E226FF12

FASTA24327,717
        10         20         30         40         50         60 
MPRYLAIVAY NGAPFRGWAK QPGYHTVQGQ IEQNLALIYQ TPINIYGSGR TDKGVHAINQ 

        70         80         90        100        110        120 
TFHVDLPDKL PLKQLIAKLN QLNYPDIFVK ALVPVAPRFH ARFAVKTKVY EYLINTRAFN 

       130        140        150        160        170        180 
PAQYHTVWQY NHPLDLAQLE ADTTLFLGRK DFWSFSTASQ KDTQRTISKI IVQQDHTGTV 

       190        200        210        220        230        240 
KLTFFGNGFL RNQIRMMVAS LVALNNRTLM RETVQQLFDQ PQKGGCKVKA PACGLYLKTV 


LYE 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes."
Hilbert H., Himmelreich R., Plagens H., Herrmann R.
Nucleic Acids Res. 24:628-639(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34795 Genomic DNA. Translation: AAC43685.1.
U00089 Genomic DNA. Translation: AAB96283.1.
PIRS62812.
RefSeqNP_109884.1. NC_000912.1.

3D structure databases

ProteinModelPortalQ50291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN196.

Proteomic databases

PaxDbQ50291.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96283; AAB96283; MPN_196.
GeneID876925.
KEGGmpn:MPN196.
PATRIC20021715. VBIMycPne110_0214.

Phylogenomic databases

eggNOGCOG0101.
KOK06173.
OMAYAMNSAL.
OrthoDBEOG6Z9B4R.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-203-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_MYCPN
AccessionPrimary (citable) accession number: Q50291
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names