Q50291 (TRUA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Synonyms:	hisT
Ordered Locus Names:	MPN_196
ORF Names:	MP635

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	tRNA pseudouridine synthase A HAMAP-Rule MF_00171		PRO_0000057413
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50291 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A5DE0B36E226FF12	FASTA	243	27,717
	10 20 30 40 50 60 MPRYLAIVAY NGAPFRGWAK QPGYHTVQGQ IEQNLALIYQ TPINIYGSGR TDKGVHAINQ 70 80 90 100 110 120 TFHVDLPDKL PLKQLIAKLN QLNYPDIFVK ALVPVAPRFH ARFAVKTKVY EYLINTRAFN 130 140 150 160 170 180 PAQYHTVWQY NHPLDLAQLE ADTTLFLGRK DFWSFSTASQ KDTQRTISKI IVQQDHTGTV 190 200 210 220 230 240 KLTFFGNGFL RNQIRMMVAS LVALNNRTLM RETVQQLFDQ PQKGGCKVKA PACGLYLKTV LYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes." Hilbert H., Himmelreich R., Plagens H., Herrmann R. Nucleic Acids Res. 24:628-639(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34795 Genomic DNA. Translation: AAC43685.1. U00089 Genomic DNA. Translation: AAB96283.1.
PIR	S62812.
RefSeq	NP_109884.1. NC_000912.1.
3D structure databases
ProteinModelPortal	Q50291.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN196.
Proteomic databases
PaxDb	Q50291.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96283; AAB96283; MPN_196.
GeneID	876925.
KEGG	mpn:MPN196.
PATRIC	20021715. VBIMycPne110_0214.
Phylogenomic databases
eggNOG	COG0101.
KO	K06173.
OMA	NYCYEYL.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-203-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_MYCPN

Accession

Primary (citable) accession number: Q50291

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program