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Protein

Probable ATP-dependent RNA helicase DDX17

Gene

Ddx17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation (By similarity). Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation.By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 143ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX17 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 17
Gene namesi
Name:Ddx17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1914290. Ddx17.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549941 – 650Probable ATP-dependent RNA helicase DDX17Add BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei444PhosphothreonineBy similarity1
Modified residuei605Omega-N-methylarginineCombined sources1

Post-translational modificationi

Sumoylation significantly increases stability, it also promotes interaction with HDAC1.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ501J6.
PaxDbiQ501J6.
PeptideAtlasiQ501J6.
PRIDEiQ501J6.

2D gel databases

REPRODUCTION-2DPAGEIPI00405364.

PTM databases

iPTMnetiQ501J6.
PhosphoSitePlusiQ501J6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000055065.
CleanExiMM_DDX17.

Interactioni

Subunit structurei

Interacts with ESR1. Interacts with NCOA1, NCOA2, NCOA3, TP53 and HDAC1. Self-associates. Interacts with DDX5 (By similarity). Interacts with DCP1A in an RNA-independent manner (By similarity). Interacts with DCP2 in an RNA-dependent manner (By similarity). Interacts with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner (By similarity). Interacts with EXOSC3 and EXOSC5 only in the presence of ZC3HAV1 in an RNA-independent manner (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Yap1P469383EBI-911206,EBI-1211949

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211894. 5 interactors.
IntActiQ501J6. 9 interactors.
MINTiMINT-1867698.
STRINGi10090.ENSMUSP00000055535.

Structurei

3D structure databases

ProteinModelPortaliQ501J6.
SMRiQ501J6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 298Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini326 – 473Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni468 – 650Transactivation domainBy similarityAdd BLAST183

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 120Q motifAdd BLAST29
Motifi246 – 249DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOVERGENiHBG015893.
InParanoidiQ501J6.
KOiK13178.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q501J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGGGFGDRD RDRDRGGFGA RGGSGLPPKK FGNPGERLRK KKWDLSELPK
60 70 80 90 100
FEKNFYVEHP EVARLTPYEV DELRRKKEIT VRGGDVCPKP VFAFHHANFP
110 120 130 140 150
QYVMDVLMDQ HFTEPTPIQC QGFPLALSGR DMVGIAQTGS GKTLAYLLPA
160 170 180 190 200
IVHINHQPYL ERGDGPICLV LAPTRELAQQ VQQVADDYGK CSRLKSTCIY
210 220 230 240 250
GGAPKGPQIR DLERGVEICI ATPGRLIDFL ESGKTNLRRC TYLVLDEADR
260 270 280 290 300
MLDMGFEPQI RKIVDQIRPD RQTLMWSATW PKEVRQLAED FLRDYTQINV
310 320 330 340 350
GNLELSANHN ILQIVDVCME SEKDHKLIQL MEEIMAEKEN KTIIFVETKR
360 370 380 390 400
RCDDLTRRMR RDGWPAMCIH GDKSQPERDW VLNEFRSGKA PILIATDVAS
410 420 430 440 450
RGLDVEDVKF VINYDYPNSS EDYVHRIGRT ARSTNKGTAY TFFTPGNLKQ
460 470 480 490 500
ARELIKVLEE ANQAINPKLM QLVDHRGGGG GGGGRSRYRT TSSANNPNLM
510 520 530 540 550
YQDECDRRLR GVKDGGRRDS TSYRDRSETD RASYANGSGY GSPNSAFGAQ
560 570 580 590 600
AGQYTYAQGT YGAAAYGTSG YTAQEYAAGT YGASSTASAG RSSQSSSQQF
610 620 630 640 650
SGIGRSGQQP QPLMSQQFAQ PPGATNMIGY MGQTAYQYPP PPPPPPPSRK
Length:650
Mass (Da):72,399
Last modified:June 7, 2005 - v1
Checksum:iE2590F3E53883D3C
GO
Isoform 2 (identifier: Q501J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-407: DVED → GLYR
     408-650: Missing.

Show »
Length:407
Mass (Da):46,364
Checksum:iB263C228210F5BAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti320E → V in AAH62910 (PubMed:15489334).Curated1
Sequence conflicti362D → Y in AAH62910 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015780404 – 407DVED → GLYR in isoform 2. 2 Publications4
Alternative sequenceiVSP_015781408 – 650Missing in isoform 2. 2 PublicationsAdd BLAST243

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039888 mRNA. Translation: BAC30474.1.
BC062910 mRNA. Translation: AAH62910.1.
BC096036 mRNA. Translation: AAH96036.1.
RefSeqiNP_001035277.1. NM_001040187.1. [Q501J6-1]
NP_951061.1. NM_199079.1. [Q501J6-2]
NP_951062.1. NM_199080.2.
UniGeneiMm.29644.

Genome annotation databases

GeneIDi67040.
KEGGimmu:67040.
UCSCiuc007wtq.1. mouse. [Q501J6-1]
uc007wtt.1. mouse. [Q501J6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK039888 mRNA. Translation: BAC30474.1.
BC062910 mRNA. Translation: AAH62910.1.
BC096036 mRNA. Translation: AAH96036.1.
RefSeqiNP_001035277.1. NM_001040187.1. [Q501J6-1]
NP_951061.1. NM_199079.1. [Q501J6-2]
NP_951062.1. NM_199080.2.
UniGeneiMm.29644.

3D structure databases

ProteinModelPortaliQ501J6.
SMRiQ501J6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211894. 5 interactors.
IntActiQ501J6. 9 interactors.
MINTiMINT-1867698.
STRINGi10090.ENSMUSP00000055535.

PTM databases

iPTMnetiQ501J6.
PhosphoSitePlusiQ501J6.

2D gel databases

REPRODUCTION-2DPAGEIPI00405364.

Proteomic databases

EPDiQ501J6.
PaxDbiQ501J6.
PeptideAtlasiQ501J6.
PRIDEiQ501J6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi67040.
KEGGimmu:67040.
UCSCiuc007wtq.1. mouse. [Q501J6-1]
uc007wtt.1. mouse. [Q501J6-2]

Organism-specific databases

CTDi10521.
MGIiMGI:1914290. Ddx17.

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
HOVERGENiHBG015893.
InParanoidiQ501J6.
KOiK13178.

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Miscellaneous databases

ChiTaRSiDdx17. mouse.
PROiQ501J6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055065.
CleanExiMM_DDX17.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX17_MOUSE
AccessioniPrimary (citable) accession number: Q501J6
Secondary accession number(s): Q6P5G1, Q8BIN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Silenced gene expression via RNA interference in PubMed:17011493 was simultaneously performed with DDX5 and DDX17; siRNA-resistant DDX5 expression was able to rescue the effect in muscle differentiation.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.