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Q501J6

- DDX17_MOUSE

UniProt

Q501J6 - DDX17_MOUSE

Protein

Probable ATP-dependent RNA helicase DDX17

Gene

Ddx17

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation By similarity. Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation.By similarity1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi136 – 1438ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. estrogen receptor binding Source: UniProtKB
    4. protein binding Source: IntAct
    5. RNA binding Source: UniProtKB-KW
    6. transcription coactivator activity Source: UniProtKB

    GO - Biological processi

    1. cardiovascular system development Source: MGI
    2. positive regulation of intracellular estrogen receptor signaling pathway Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    4. post-embryonic development Source: MGI
    5. regulation of skeletal muscle cell differentiation Source: UniProtKB
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX17 (EC:3.6.4.13)
    Alternative name(s):
    DEAD box protein 17
    Gene namesi
    Name:Ddx17
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1914290. Ddx17.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 650650Probable ATP-dependent RNA helicase DDX17PRO_0000054994Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki50 – 50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei444 – 4441PhosphothreonineBy similarity

    Post-translational modificationi

    Sumoylation significantly increases stability, it also promotes interaction with HDAC1.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ501J6.
    PaxDbiQ501J6.
    PRIDEiQ501J6.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00405364.

    PTM databases

    PhosphoSiteiQ501J6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ501J6.
    BgeeiQ501J6.
    CleanExiMM_DDX17.
    GenevestigatoriQ501J6.

    Interactioni

    Subunit structurei

    Interacts with ESR1. Interacts with NCOA1, NCOA2, NCOA3, TP53 and HDAC1. Self-associates. Interacts with DDX5 By similarity. Interacts with DCP1A in an RNA-independent manner By similarity. Interacts with DCP2 in an RNA-dependent manner By similarity. Interacts with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner By similarity. Interacts with EXOSC3 and EXOSC5 only in the presence of ZC3HAV1 in an RNA-independent manner By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Yap1P469383EBI-911206,EBI-1211949

    Protein-protein interaction databases

    BioGridi211894. 11 interactions.
    IntActiQ501J6. 8 interactions.
    MINTiMINT-1867698.

    Structurei

    3D structure databases

    ProteinModelPortaliQ501J6.
    SMRiQ501J6. Positions 47-467.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini123 – 298176Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini326 – 473148Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni468 – 650183Transactivation domainBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi92 – 12029Q motifAdd
    BLAST
    Motifi246 – 2494DEAD box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOVERGENiHBG015893.
    InParanoidiQ501J6.
    KOiK13178.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q501J6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRGGGFGDRD RDRDRGGFGA RGGSGLPPKK FGNPGERLRK KKWDLSELPK    50
    FEKNFYVEHP EVARLTPYEV DELRRKKEIT VRGGDVCPKP VFAFHHANFP 100
    QYVMDVLMDQ HFTEPTPIQC QGFPLALSGR DMVGIAQTGS GKTLAYLLPA 150
    IVHINHQPYL ERGDGPICLV LAPTRELAQQ VQQVADDYGK CSRLKSTCIY 200
    GGAPKGPQIR DLERGVEICI ATPGRLIDFL ESGKTNLRRC TYLVLDEADR 250
    MLDMGFEPQI RKIVDQIRPD RQTLMWSATW PKEVRQLAED FLRDYTQINV 300
    GNLELSANHN ILQIVDVCME SEKDHKLIQL MEEIMAEKEN KTIIFVETKR 350
    RCDDLTRRMR RDGWPAMCIH GDKSQPERDW VLNEFRSGKA PILIATDVAS 400
    RGLDVEDVKF VINYDYPNSS EDYVHRIGRT ARSTNKGTAY TFFTPGNLKQ 450
    ARELIKVLEE ANQAINPKLM QLVDHRGGGG GGGGRSRYRT TSSANNPNLM 500
    YQDECDRRLR GVKDGGRRDS TSYRDRSETD RASYANGSGY GSPNSAFGAQ 550
    AGQYTYAQGT YGAAAYGTSG YTAQEYAAGT YGASSTASAG RSSQSSSQQF 600
    SGIGRSGQQP QPLMSQQFAQ PPGATNMIGY MGQTAYQYPP PPPPPPPSRK 650
    Length:650
    Mass (Da):72,399
    Last modified:June 7, 2005 - v1
    Checksum:iE2590F3E53883D3C
    GO
    Isoform 2 (identifier: Q501J6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         404-407: DVED → GLYR
         408-650: Missing.

    Show »
    Length:407
    Mass (Da):46,364
    Checksum:iB263C228210F5BAF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti320 – 3201E → V in AAH62910. (PubMed:15489334)Curated
    Sequence conflicti362 – 3621D → Y in AAH62910. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei404 – 4074DVED → GLYR in isoform 2. 2 PublicationsVSP_015780
    Alternative sequencei408 – 650243Missing in isoform 2. 2 PublicationsVSP_015781Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK039888 mRNA. Translation: BAC30474.1.
    BC062910 mRNA. Translation: AAH62910.1.
    BC096036 mRNA. Translation: AAH96036.1.
    RefSeqiNP_001035277.1. NM_001040187.1. [Q501J6-1]
    NP_951061.1. NM_199079.1. [Q501J6-2]
    NP_951062.1. NM_199080.2.
    UniGeneiMm.29644.

    Genome annotation databases

    GeneIDi67040.
    KEGGimmu:67040.
    UCSCiuc007wtq.1. mouse. [Q501J6-1]
    uc007wtt.1. mouse. [Q501J6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK039888 mRNA. Translation: BAC30474.1 .
    BC062910 mRNA. Translation: AAH62910.1 .
    BC096036 mRNA. Translation: AAH96036.1 .
    RefSeqi NP_001035277.1. NM_001040187.1. [Q501J6-1 ]
    NP_951061.1. NM_199079.1. [Q501J6-2 ]
    NP_951062.1. NM_199080.2.
    UniGenei Mm.29644.

    3D structure databases

    ProteinModelPortali Q501J6.
    SMRi Q501J6. Positions 47-467.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211894. 11 interactions.
    IntActi Q501J6. 8 interactions.
    MINTi MINT-1867698.

    PTM databases

    PhosphoSitei Q501J6.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00405364.

    Proteomic databases

    MaxQBi Q501J6.
    PaxDbi Q501J6.
    PRIDEi Q501J6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 67040.
    KEGGi mmu:67040.
    UCSCi uc007wtq.1. mouse. [Q501J6-1 ]
    uc007wtt.1. mouse. [Q501J6-2 ]

    Organism-specific databases

    CTDi 10521.
    MGIi MGI:1914290. Ddx17.

    Phylogenomic databases

    eggNOGi COG0513.
    HOVERGENi HBG015893.
    InParanoidi Q501J6.
    KOi K13178.

    Miscellaneous databases

    ChiTaRSi DDX17. mouse.
    NextBioi 323386.
    PROi Q501J6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q501J6.
    Bgeei Q501J6.
    CleanExi MM_DDX17.
    Genevestigatori Q501J6.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain and Eye.
    3. "The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation."
      Caretti G., Schiltz R.L., Dilworth F.J., Di Padova M., Zhao P., Ogryzko V., Fuller-Pace F.V., Hoffman E.P., Tapscott S.J., Sartorelli V.
      Dev. Cell 11:547-560(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDDX17_MOUSE
    AccessioniPrimary (citable) accession number: Q501J6
    Secondary accession number(s): Q6P5G1, Q8BIN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Silenced gene expression via RNA interference in PubMed:17011493 was simultaneously performed with DDX5 and DDX17; siRNA-resistant DDX5 expression was able to rescue the effect in muscle differentiation.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3