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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose and in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Probably also able to use ADP-Glc, CDP-Glc, GDP-Glc and TDP-Glc as glucosyl donors.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity
Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28D-glucose 6-phosphateBy similarity1
Binding sitei106D-glucose 6-phosphateBy similarity1
Sitei115Involved in alpha anomer selectivityBy similarity1
Binding sitei160D-glucose 6-phosphateBy similarity1
Sitei185Involved in alpha anomer selectivityBy similarity1
Binding sitei302UDP-glucoseBy similarity1
Binding sitei307UDP-glucoseBy similarity1
Binding sitei340D-glucose 6-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:ML2254
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2254.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003489071 – 498Trehalose-6-phosphate synthaseAdd BLAST498

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272631.ML2254.

Structurei

3D structure databases

ProteinModelPortaliQ50167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 49UDP-glucose bindingBy similarity2
Regioni405 – 409UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191477.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiPOG093I018P.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.

Sequencei

Sequence statusi: Complete.

Q50167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRGNHGSK TSSDKHLGDS DFVVVANRLP VDQVRLPDGT AIWKRSPGGL
60 70 80 90 100
VTALEPLLRQ RRGAWVGWPG VINDNVDLDL TIKSIVQDGL TLYPVRLNTH
110 120 130 140 150
DVAEYYEGFS NATLWPLYHD VIVKPIYHCE WWERYVDVNR RFAETTSRTA
160 170 180 190 200
AYGGTVWVQD YQLQLVPKML RIMRPDLTIG FFLHIPFPPV ELFMQIPWRT
210 220 230 240 250
EIIEGLLGAD LVGFHLTSGA QNFLFLSRHL LGANTSRGLV GVRSRFGEVQ
260 270 280 290 300
LKSHTVQVGA FPISIDSKEI DQATRDRNVR RRAREIRAEL GNPRKILLGV
310 320 330 340 350
DRLDYTKGID VRLRAFAELL AEGRAKRDDT VLVQLATPSR ERVESYKILR
360 370 380 390 400
NDIERQVGHI NGEYGEVGHP VVHYLHRPIP RDELIAFYVA SDVMLVTPLR
410 420 430 440 450
DGMNLVAKEY VACRNDLGGA LVLSEFTGAA AELRQAYLVN PHDLEGVKDT
460 470 480 490
IEAALNQLAE EARRRMRSLR RQVLAHDVDR WARSFLDALA EAPARDAT
Length:498
Mass (Da):56,300
Last modified:November 1, 1996 - v1
Checksum:iC131B6165655D668
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15187 Genomic DNA. Translation: AAA63137.1.
AL583924 Genomic DNA. Translation: CAC31210.1.
PIRiB87191.
RefSeqiNP_302467.1. NC_002677.1.
WP_010908787.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31210; CAC31210; CAC31210.
GeneIDi908776.
KEGGimle:ML2254.
PATRICi18058777. VBIMycLep78757_4306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15187 Genomic DNA. Translation: AAA63137.1.
AL583924 Genomic DNA. Translation: CAC31210.1.
PIRiB87191.
RefSeqiNP_302467.1. NC_002677.1.
WP_010908787.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ50167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2254.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31210; CAC31210; CAC31210.
GeneIDi908776.
KEGGimle:ML2254.
PATRICi18058777. VBIMycLep78757_4306.

Organism-specific databases

LepromaiML2254.

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191477.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiPOG093I018P.

Enzyme and pathway databases

UniPathwayiUPA00299.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOTSA_MYCLE
AccessioniPrimary (citable) accession number: Q50167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1996
Last modified: February 15, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.