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Q50144 (PUR2_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:ML2235
ORF Names:MLCB5.08
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151463

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q50144 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C5118E1CA84E7018

FASTA42243,984
        10         20         30         40         50         60 
MRVLVIGSGA REHALLLALS RDPQVKGLVV APGNAGTARL AEQYDVDISS GEDIVALARD 

        70         80         90        100        110        120 
VRADMVVIGP ELPLVLGVAD AVRAAGIVCF GPSKDAARIE GSKAFAKEVM AAAGVRTSRS 

       130        140        150        160        170        180 
EIVNSPARLD AALDRFGPPV GDLSWVVKDD RLAAGKGVVV TSDRDVARTH AAGLLEAGHP 

       190        200        210        220        230        240 
VLLESYLDGP EVSLFCVVDG RTVLPLLPAQ DFKRVGEGDT GPNTGGMGAY APLPWLPDEV 

       250        260        270        280        290        300 
CQQALTSIVE PVAAELVQRG SPFRGLLYVG LAVTASGPAV VEFNCRFGDP ETQVVLALLD 

       310        320        330        340        350        360 
SPLGQLLYAA GTGSLADFGE LHWRSGAAVA VVLAAENYPR RPRVGDIVFG SEIEGVLHAG 

       370        380        390        400        410        420 
TTRRDDGVIV SSGGRVLSVV ATGDDLSAAR SQAYRIIGSI RLLGSHFRKD IGFSAAKGRI 


HI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15187 Genomic DNA. Translation: AAA63135.1.
Z95151 Genomic DNA. Translation: CAB08419.1.
AL583924 Genomic DNA. Translation: CAC31191.1.
PIRG87188.
RefSeqNP_302459.1. NC_002677.1.

3D structure databases

ProteinModelPortalQ50144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML2235.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31191; CAC31191; CAC31191.
GeneID908797.
KEGGmle:ML2235.
PATRIC18058655. VBIMycLep78757_4246.

Organism-specific databases

LepromaML2235.
CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_MYCLE
AccessionPrimary (citable) accession number: Q50144
Secondary accession number(s): O05742
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways