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Protein

Diaminopimelate decarboxylase

Gene

lysA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.UniRule annotation

Catalytic activityi

Meso-2,6-diaminoheptanedioate = L-lysine + CO2.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate decarboxylase (lysA)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei283 – 2831Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei328 – 3281SubstrateUniRule annotation
Binding sitei369 – 3691SubstrateUniRule annotation
Binding sitei373 – 3731SubstrateUniRule annotation
Active sitei400 – 4001Proton donorSequence analysis
Binding sitei401 – 4011SubstrateUniRule annotation
Binding sitei430 – 4301Pyridoxal phosphateUniRule annotation
Binding sitei430 – 4301SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00034; UER00027.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate decarboxylaseUniRule annotation (EC:4.1.1.20UniRule annotation)
Short name:
DAP decarboxylaseUniRule annotation
Short name:
DAPDCUniRule annotation
Gene namesi
Name:lysAUniRule annotation
Ordered Locus Names:ML1128
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1128.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Diaminopimelate decarboxylasePRO_0000149928Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1128.

Structurei

3D structure databases

ProteinModelPortaliQ50140.
SMRiQ50140. Positions 28-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 3284Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CU5. Bacteria.
COG0019. LUCA.
HOGENOMiHOG000045071.
KOiK01586.
OMAiLKGNKFG.
OrthoDBiPOG091H013X.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_02120. LysA. 1 hit.
InterProiIPR009006. Ala_racemase/Decarboxylase_C.
IPR002986. DAP_deCOOHase_LysA.
IPR022643. De-COase2_C.
IPR022644. De-COase2_N.
IPR022653. De-COase2_pyr-phos_BS.
IPR000183. Orn/DAP/Arg_de-COase.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF02784. Orn_Arg_deC_N. 1 hit.
PF00278. Orn_DAP_Arg_deC. 1 hit.
[Graphical view]
PRINTSiPR01181. DAPDCRBXLASE.
PR01179. ODADCRBXLASE.
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR01048. lysA. 1 hit.
PROSITEiPS00878. ODR_DC_2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVHTAGPRH AEKTRHTATP QRVQPSDDLL RLASNVWPRN ITRDETGVAC
60 70 80 90 100
IAGNKLTDLA GEYGTPLFVI DEDDFRFRCR EIAAAFGGGE NVHYAAKAFL
110 120 130 140 150
CTEIARWIDE EGLSLDVCSG GELAVALHAS FPPERISLHG NNKSVAELKD
160 170 180 190 200
AVKAGVGYIV LDSTTEIERL DAIAGEAGIV QDVLVRLTVG VEAHTHEFIA
210 220 230 240 250
TAHEDQKFGL SVASGAAMAA VRRVFATDNL RLVGLHSHIG SQIFDVAGFE
260 270 280 290 300
LAAHRVIGLL CDIVGEFDPE KTAQLSIVDL GGGLGISYLP DDDPPPIFEL
310 320 330 340 350
AAKLGAIVSN ESAAVGLPVP KLMVEPGRAI AGPGTITLYE VGTIKDVDVS
360 370 380 390 400
ATAHRRYVSI DGGMSDNIRT ALYDAQYDVR LVSRTSDAPA APASIVGKHC
410 420 430 440 450
ESGDIVVRDT WVPDDLKPGD LVGVAATGAY CYSLSSRYNM LGRPAVVAVC
460 470
AGQARLILRR ETVDDLLSLE VR
Length:472
Mass (Da):50,278
Last modified:April 27, 2001 - v2
Checksum:iDB234E82D8274B39
GO

Sequence cautioni

The sequence AAA63102 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15186 Genomic DNA. Translation: AAA63102.1. Different initiation.
AL583920 Genomic DNA. Translation: CAC31509.1.
PIRiB87050.
RefSeqiNP_301822.1. NC_002677.1.
WP_010908146.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31509; CAC31509; CAC31509.
GeneIDi910222.
KEGGimle:ML1128.
PATRICi18054211. VBIMycLep78757_2050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15186 Genomic DNA. Translation: AAA63102.1. Different initiation.
AL583920 Genomic DNA. Translation: CAC31509.1.
PIRiB87050.
RefSeqiNP_301822.1. NC_002677.1.
WP_010908146.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ50140.
SMRiQ50140. Positions 28-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31509; CAC31509; CAC31509.
GeneIDi910222.
KEGGimle:ML1128.
PATRICi18054211. VBIMycLep78757_2050.

Organism-specific databases

LepromaiML1128.

Phylogenomic databases

eggNOGiENOG4105CU5. Bacteria.
COG0019. LUCA.
HOGENOMiHOG000045071.
KOiK01586.
OMAiLKGNKFG.
OrthoDBiPOG091H013X.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00027.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_02120. LysA. 1 hit.
InterProiIPR009006. Ala_racemase/Decarboxylase_C.
IPR002986. DAP_deCOOHase_LysA.
IPR022643. De-COase2_C.
IPR022644. De-COase2_N.
IPR022653. De-COase2_pyr-phos_BS.
IPR000183. Orn/DAP/Arg_de-COase.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF02784. Orn_Arg_deC_N. 1 hit.
PF00278. Orn_DAP_Arg_deC. 1 hit.
[Graphical view]
PRINTSiPR01181. DAPDCRBXLASE.
PR01179. ODADCRBXLASE.
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR01048. lysA. 1 hit.
PROSITEiPS00878. ODR_DC_2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCDA_MYCLE
AccessioniPrimary (citable) accession number: Q50140
Secondary accession number(s): Q9CC78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.