Reviewed, UniProtKB/Swiss-Prot Q500W7 (PIGM_ARATH)

Names and origin

Protein names

Recommended name:
    GPI mannosyltransferase 1
    EC=2.4.1.-
Alternative name(s):
    GPI mannosyltransferase I
      Short name=GPI-MT-I
    Phosphatidylinositol-glycan biosynthesis class M protein
      Short name=PIG-M
    Protein PEANUT 1

Gene names

Name:	PIGM
Synonyms:	PNT1
Ordered Locus Names:	At5g22130
ORF Names:	T6G21.34

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Ref.3
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in influorescence meristem. Present at low level in roots, hypocotyls, leaves and stems. Ref.3
Disruption phenotype	Plants display cell walls with decreased crystalline cellulose, increased pectins and irregular and ectopic deposition of pectins, xyloglucans and callose. Ref.3
Sequence similarities	Belongs to the PIGM family.
Sequence caution	The sequence CAC34506.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Ref.3 Inferred from mutant phenotype. Source: TAIR cell division Ref.3 Inferred from mutant phenotype. Source: TAIR cellulose biosynthetic process Ref.3 Inferred from mutant phenotype. Source: TAIR embryonic development Ref.3 Inferred from mutant phenotype. Source: TAIR plant-type cell wall biogenesis Ref.3 Inferred from mutant phenotype. Source: TAIR
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	GPI mannosyltransferase 1		PRO_0000246222
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Potential
	Topological domain	42 – 93	52	Lumenal Potential
	Transmembrane	94 – 114	21	Potential
	Topological domain	115 – 121	7	Cytoplasmic Potential
	Transmembrane	122 – 142	21	Potential
	Topological domain	143 – 148	6	Lumenal Potential
	Transmembrane	149 – 169	21	Potential
	Topological domain	170 – 173	4	Cytoplasmic Potential
	Transmembrane	174 – 194	21	Potential
	Topological domain	195 – 243	49	Lumenal Potential
	Transmembrane	244 – 264	21	Potential
	Topological domain	265 – 314	50	Cytoplasmic Potential
	Transmembrane	315 – 335	21	Potential
	Topological domain	336 – 345	10	Lumenal Potential
	Transmembrane	346 – 366	21	Potential
	Topological domain	367 – 374	8	Cytoplasmic Potential
	Transmembrane	375 – 395	21	Potential
	Topological domain	396 – 401	6	Lumenal Potential
	Transmembrane	402 – 422	21	Potential
	Topological domain	423 – 450	28	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q500W7-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 4CD10F65C9CA8056	FASTA	450	52,946
	10 20 30 40 50 60 MSRESENTDD EPSKWMKFRS LLVFSMLLRV FLIVYGEWQD AHMEVRYTDV DYIVFSDAAS 70 80 90 100 110 120 LMASGESPYK RTTYRYSPLL ALLLTPNSFF HRSWGKFLFS ASDLLVGWFI HKILKQRKVP 130 140 150 160 170 180 EKICTYSVMV WLFNPFTFTI GTRGNCEPIV CAMILWIILC LMQGNLLQAA FWYGLVVHFR 190 200 210 220 230 240 VYPIIYALPI ILVLDTQVFR SGQKPALLYW NTGQAKTPAS NMERKTFLFN LLTTLKSLFS 250 260 270 280 290 300 RERIMFALIS GGVFLACNAV SFYFYGQEFL HEALLYHLTR TDPRHNFSIY FYHIYLHYER 310 320 330 340 350 360 QFSAVEKLIS FLPQFTVQFA LVFCFSQDLV FCIFLQTVAF VTFNKVITAQ YFVWFYCLLP 370 380 390 400 410 420 LILPWSHMKL KWEGLLCIIM WIGAQTHWLL WGYMLEFKGV NVFLPLWIAS LLFLAANTFV 430 440 450 LVRIIQRHRF SPLFRRYESS SSSNNVTKED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis cDNA clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Glycosylphosphatidylinositol-anchored proteins are required for cell wall synthesis and morphogenesis in Arabidopsis." Gillmor C.S., Lukowitz W., Brininstool G., Sedbrook J.C., Hamann T., Poindexter P., Somerville C. Plant Cell 17:1128-1140(2005) [PubMed: 15772281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-450, FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL589883 Genomic DNA. Translation: CAC34506.1. Sequence problems. BT022100 mRNA. Translation: AAY34161.1. AY302133 mRNA. Translation: AAP59446.1. Different initiation.
IPI	IPI00526341.
RefSeq	NP_680199.1.
UniGene	At.45632
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q500W7.
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Proteomic databases
PRIDE	Q500W7.
Genome annotation databases
GeneID	832274.
GenomeReviews	Gene locus AT5G22130 in contig BA000015_GR.
KEGG	ath:AT5G22130.
NMPDR	fig|3702.1.peg.24369.
Organism-specific databases
TAIR	At5g22130.
Phylogenomic databases
HOGENOM	HBG559139.
InParanoid	Q500W7.
OMA	NFSPYFY.
Gene expression databases
Genevestigator	Q500W7.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
PANTHER	PTHR12886. Mannos_trans_DXD. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIGM_ARATH

Accession

Primary (citable) accession number: Q500W7
Secondary accession number(s): Q7XZS2, Q9C575

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 7, 2005
Last modified:	December 15, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)