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Q500W7 (PIGM_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI mannosyltransferase 1
EC=2.4.1.-
Alternative name(s):
GPI mannosyltransferase I
Short name=GPI-MT-I
Phosphatidylinositol-glycan biosynthesis class M protein
Short name=PIG-M
Protein PEANUT 1
Gene names
Name:PIGM
Synonyms:PNT1
Ordered Locus Names:At5g22130
ORF Names:T6G21.34
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Ref.4

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in influorescence meristem. Present at low level in roots, hypocotyls, leaves and stems. Ref.4

Disruption phenotype

Plants display cell walls with decreased crystalline cellulose, increased pectins and irregular and ectopic deposition of pectins, xyloglucans and callose. Ref.4

Sequence similarities

Belongs to the PIGM family.

Sequence caution

The sequence AAP59446.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC34506.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q500W7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450GPI mannosyltransferase 1
PRO_0000246222

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 9352Lumenal Potential
Transmembrane94 – 11421Helical; Potential
Topological domain115 – 1217Cytoplasmic Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 1486Lumenal Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 1734Cytoplasmic Potential
Transmembrane174 – 19421Helical; Potential
Topological domain195 – 24349Lumenal Potential
Transmembrane244 – 26421Helical; Potential
Topological domain265 – 31450Cytoplasmic Potential
Transmembrane315 – 33521Helical; Potential
Topological domain336 – 34510Lumenal Potential
Transmembrane346 – 36621Helical; Potential
Topological domain367 – 3748Cytoplasmic Potential
Transmembrane375 – 39521Helical; Potential
Topological domain396 – 4016Lumenal Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 45028Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 4CD10F65C9CA8056

FASTA45052,946
        10         20         30         40         50         60 
MSRESENTDD EPSKWMKFRS LLVFSMLLRV FLIVYGEWQD AHMEVRYTDV DYIVFSDAAS 

        70         80         90        100        110        120 
LMASGESPYK RTTYRYSPLL ALLLTPNSFF HRSWGKFLFS ASDLLVGWFI HKILKQRKVP 

       130        140        150        160        170        180 
EKICTYSVMV WLFNPFTFTI GTRGNCEPIV CAMILWIILC LMQGNLLQAA FWYGLVVHFR 

       190        200        210        220        230        240 
VYPIIYALPI ILVLDTQVFR SGQKPALLYW NTGQAKTPAS NMERKTFLFN LLTTLKSLFS 

       250        260        270        280        290        300 
RERIMFALIS GGVFLACNAV SFYFYGQEFL HEALLYHLTR TDPRHNFSIY FYHIYLHYER 

       310        320        330        340        350        360 
QFSAVEKLIS FLPQFTVQFA LVFCFSQDLV FCIFLQTVAF VTFNKVITAQ YFVWFYCLLP 

       370        380        390        400        410        420 
LILPWSHMKL KWEGLLCIIM WIGAQTHWLL WGYMLEFKGV NVFLPLWIAS LLFLAANTFV 

       430        440        450 
LVRIIQRHRF SPLFRRYESS SSSNNVTKED

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis cDNA clones."
Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Glycosylphosphatidylinositol-anchored proteins are required for cell wall synthesis and morphogenesis in Arabidopsis."
Gillmor C.S., Lukowitz W., Brininstool G., Sedbrook J.C., Hamann T., Poindexter P., Somerville C.
Plant Cell 17:1128-1140(2005) [PubMed: 15772281] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-450, FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL589883 Genomic DNA. Translation: CAC34506.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92986.1.
BT022100 mRNA. Translation: AAY34161.1.
AY302133 mRNA. Translation: AAP59446.1. Different initiation.
IPIIPI00526341.
RefSeqNP_680199.1. NM_147894.4.
UniGeneAt.45632.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ500W7.

Protein family/group databases

CAZyGT50. Glycosyltransferase Family 50.

Proteomic databases

PRIDEQ500W7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G22130.1; AT5G22130.1; AT5G22130.
GeneID832274.
GenomeReviewsGene locus AT5G22130 in contig BA000015_GR.
KEGGath:AT5G22130.
NMPDRfig|3702.1.peg.24369.

Organism-specific databases

TAIRAt5g22130.

Phylogenomic databases

GeneTreeEPGT00050000019037.
HOGENOMHBG559139.
InParanoidQ500W7.
OMAEFLEHTY.
PhylomeDBQ500W7.
ProtClustDBPLN02841.

Gene expression databases

GenevestigatorQ500W7.

Family and domain databases

InterProIPR007704. Mannosyltransferase_DXD.
[Graphical view]
KOK05284.
PANTHERPTHR12886. Mannos_trans_DXD. 1 hit.
PfamPF05007. Mannosyl_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePIGM_ARATH
AccessionPrimary (citable) accession number: Q500W7
Secondary accession number(s): Q7XZS2, Q9C575
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 7, 2005
Last modified: November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents