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Protein

Nitrilase

Gene

Psyr_0007

Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Arylacetonitrilase which is capable of hydrolyzing indole-3-acetonitrile (IAN) to the plant hormone indole-3-acetate (IAA), and allows the plant pathogenic bacterium to use IAN as a sole nitrogen source. Is also able to hydrolyze phenylpropionitrile (PPN), allowing the use of this compound as a sole nitrogen source. This enzyme may represent an additional mechanism for IAA biosynthesis or may be used to degrade and assimilate aldoximes and nitriles produced during host plant secondary metabolism.1 Publication

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation1 Publication
Indole-3-acetonitrile + 2 H2O = indole-3-acetate + NH3.1 Publication
Phenylpropionitrile + 2 H2O = phenylpropionate + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorPROSITE-ProRule annotation1
Active sitei127Proton donorPROSITE-ProRule annotation1
Active sitei161NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciPSYR205918:GJ94-7-MONOMER.
BRENDAi3.5.5.7. 12469.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase (EC:3.5.5.1)
Alternative name(s):
Arylacetonitrilase
Indole-3-acetonitrile hydrolase
Short name:
IAN hydrolase
Short name:
Indole-3-acetonitrilase
Gene namesi
Ordered Locus Names:Psyr_0007
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
Proteomesi
  • UP000000426 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to grow in medium in which the sole nitrogen source is either indole-3-acetonitrile (IAN) or phenylpropionitrile (PPN). Their growth is also moderately reduced in medium with ammonia as the nitrogen source; one possible explanation for this phenotype is that the disruption of Psyr_0007 results in the accumulation of endogenous nitriles that can no longer be degraded and which inhibit bacterial growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004257121 – 336NitrilaseAdd BLAST336

Interactioni

Protein-protein interaction databases

STRINGi205918.Psyr_0007.

Structurei

3D structure databases

ProteinModelPortaliQ500U1.
SMRiQ500U1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 278CN hydrolasePROSITE-ProRule annotationAdd BLAST274

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105ES4. Bacteria.
COG0388. LUCA.
HOGENOMiHOG000256364.
KOiK01502.
OMAiWPSFSLY.
OrthoDBiPOG091H0LO0.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q500U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEPLKVACV QAAPVFLDLD ATVDKTITLM EQAAAAGAGL IAFPETWIPG
60 70 80 90 100
YPWFLWLDAP AWNMPLVQRY HQQSLVLDSV QARRISDAAR HLGLYVVLGY
110 120 130 140 150
SERNKASLYI GQWIIDDHGE TVGVRRKLKA THVERTMFGE GDGASLRTFE
160 170 180 190 200
TPVGVLGALC CWEHLQPLSK YAMYAQNEQI HVAAWPSFSL YRNATSALGP
210 220 230 240 250
EVNTAASRVY AAEGQCFVLA PCAIVSPEMI EMLCDSDAKR SLLQAGGGHA
260 270 280 290 300
RIFGPDGSDL ATPLGEHEEG LLYATLDPAA LTLAKVAADP AGHYSRPDVT
310 320 330
RLMFNPNPTP CVVDLPDLPI SSESIELLRP DIALEV
Length:336
Mass (Da):36,553
Last modified:June 7, 2005 - v1
Checksum:iEC3DF2A762E37729
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000075 Genomic DNA. Translation: AAY35081.1.
RefSeqiWP_011266126.1. NC_007005.1.
YP_233119.1. NC_007005.1.

Genome annotation databases

EnsemblBacteriaiAAY35081; AAY35081; Psyr_0007.
GeneIDi3365482.
KEGGipsb:Psyr_0007.
PATRICifig|205918.7.peg.7.

Similar proteinsi

Entry informationi

Entry nameiNITR_PSEU2
AccessioniPrimary (citable) accession number: Q500U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: June 7, 2005
Last modified: October 25, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families