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Q500B6

- DAPF_PSEU2

UniProt

Q500B6 - DAPF_PSEU2

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Protein
Diaminopimelate epimerase
Gene
dapF, Psyr_0183
Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Substrate By similarity
Binding sitei46 – 461Substrate By similarity
Binding sitei66 – 661Substrate By similarity
Active sitei75 – 751Proton donor/acceptor By similarity
Binding sitei159 – 1591Substrate By similarity
Sitei161 – 1611Important for catalytic activity By similarity
Binding sitei192 – 1921Substrate By similarity
Sitei210 – 2101Important for catalytic activity By similarity
Active sitei219 – 2191Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciPSYR205918:GJ94-184-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:Psyr_0183
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
ProteomesiUP000000426: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Diaminopimelate epimeraseUniRule annotation
PRO_1000011936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 219UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi205918.Psyr_0183.

Structurei

3D structure databases

ProteinModelPortaliQ500B6.
SMRiQ500B6. Positions 3-276.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Substrate binding By similarity
Regioni75 – 773Substrate binding By similarity
Regioni210 – 2112Substrate binding By similarity
Regioni220 – 2212Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiVFDRYFL.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q500B6-1 [UniParc]FASTAAdd to Basket

« Hide

MLLRFTKMHG LGNDFMVLDL VSQHAHILPK HAKQWGDRHT GIGFDQLLLV    50
EAPNNPDVDF RYRIFNSDGS EVEQCGNGAR CFARFVLDKR LTAKKQIRVE 100
TKSGIIELDI RSDGQISVDM GPPRFVPEEI PFEAAEQATC YTVDVDGQHV 150
DMAAVSMGNP HAVLRVDDIN NAPVHELGPK IEHHPRFPAR VNVGFLHVVD 200
RQRAQLRVWE RGAGETQACG TGACAAAVAA ISQGWMDSPL LIDLPGGRLS 250
IEWAGPGHSV MMTGPAVRVY EGQVRL 276
Length:276
Mass (Da):30,399
Last modified:June 7, 2005 - v1
Checksum:iDE1C20A3AE921DAA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY35256.1.
RefSeqiWP_003401768.1. NC_007005.1.
YP_233294.1. NC_007005.1.

Genome annotation databases

EnsemblBacteriaiAAY35256; AAY35256; Psyr_0183.
GeneIDi3365659.
KEGGipsb:Psyr_0183.
PATRICi19980747. VBIPseSyr42314_0179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY35256.1 .
RefSeqi WP_003401768.1. NC_007005.1.
YP_233294.1. NC_007005.1.

3D structure databases

ProteinModelPortali Q500B6.
SMRi Q500B6. Positions 3-276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205918.Psyr_0183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY35256 ; AAY35256 ; Psyr_0183 .
GeneIDi 3365659.
KEGGi psb:Psyr_0183.
PATRICi 19980747. VBIPseSyr42314_0179.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi VFDRYFL.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci PSYR205918:GJ94-184-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B728a.

Entry informationi

Entry nameiDAPF_PSEU2
AccessioniPrimary (citable) accession number: Q500B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 7, 2005
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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