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Protein

Probable tRNA-dihydrouridine synthase

Gene

dus

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.By similarity

Catalytic activityi

5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84FMNBy similarity1
Active sitei115Proton donorBy similarity1
Binding sitei157FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 6FMNBy similarity3
Nucleotide bindingi219 – 221FMNBy similarity3
Nucleotide bindingi243 – 244FMNBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN, NADP, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-dihydrouridine synthase (EC:1.3.1.-)
Gene namesi
Name:dus
Ordered Locus Names:ML2186
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2186.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621361 – 384Probable tRNA-dihydrouridine synthaseAdd BLAST384

Interactioni

Protein-protein interaction databases

STRINGi272631.ML2186.

Structurei

3D structure databases

ProteinModelPortaliQ50049.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family.Curated

Phylogenomic databases

eggNOGiENOG4105CEH. Bacteria.
COG0042. LUCA.
HOGENOMiHOG000217856.
OMAiTIKMRKG.
OrthoDBiPOG091H02GM.

Family and domain databases

Gene3Di1.10.1200.80. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR024036. tRNA-dHydroUridine_Synthase_C.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 2 hits.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
TIGRFAMsiTIGR00737. nifR3_yhdG. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAPMAGVTN VTFRTLCREF GSTRGNLLRP APPHLRSPLE QSQVGTISGL
60 70 80 90 100
YVCEMVTARA LVERNAVTIH MTTFAPDESP RSLQLYTVDP ATTYTAAKMV
110 120 130 140 150
ADEGLADHID MNFGCPMPKV TRRGGGAALP YKRRLFGQIV AAAVRATEGT
160 170 180 190 200
KIPVTVKFRI GIDDEHHTHL DAGRIAEAEG AAAVALHART AAQRYSGTAD
210 220 230 240 250
WAQIAQLKQQ VRTIPVLGNG DIYDASDALA MMAVTGCDGV VIGRGCLGRP
260 270 280 290 300
WLFAELSAAF TGRPAPAPPT LGEVAEIVRR HGKLLVAHFG EDKGMRDIRK
310 320 330 340 350
HIAWYLHGFP AGSELRNALA LVKTLDELDC LLNRLDGAVP FPDAAIGPRG
360 370 380
RQGSPAWVAL PDSWLTDPDD CTVPIGADIM ESGG
Length:384
Mass (Da):41,100
Last modified:November 1, 1996 - v1
Checksum:i44B6D0B1D678E43C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15182 Genomic DNA. Translation: AAA62994.1.
AL583924 Genomic DNA. Translation: CAC31141.1.
PIRiE87182.
RefSeqiNP_302432.1. NC_002677.1.
WP_010908752.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31141; CAC31141; CAC31141.
GeneIDi909337.
KEGGimle:ML2186.
PATRICi18058453. VBIMycLep78757_4145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15182 Genomic DNA. Translation: AAA62994.1.
AL583924 Genomic DNA. Translation: CAC31141.1.
PIRiE87182.
RefSeqiNP_302432.1. NC_002677.1.
WP_010908752.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ50049.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31141; CAC31141; CAC31141.
GeneIDi909337.
KEGGimle:ML2186.
PATRICi18058453. VBIMycLep78757_4145.

Organism-specific databases

LepromaiML2186.

Phylogenomic databases

eggNOGiENOG4105CEH. Bacteria.
COG0042. LUCA.
HOGENOMiHOG000217856.
OMAiTIKMRKG.
OrthoDBiPOG091H02GM.

Family and domain databases

Gene3Di1.10.1200.80. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR024036. tRNA-dHydroUridine_Synthase_C.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 2 hits.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
TIGRFAMsiTIGR00737. nifR3_yhdG. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS_MYCLE
AccessioniPrimary (citable) accession number: Q50049
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.