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Q50028

- PUR1_MYCLE

UniProt

Q50028 - PUR1_MYCLE

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Protein
Amidophosphoribosyltransferase
Gene
purF, ML2206, MLCB5.38
Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581For GATase activity By similarity
Metal bindingi299 – 2991Iron-sulfur (4Fe-4S) By similarity
Metal bindingi346 – 3461Magnesium By similarity
Metal bindingi408 – 4081Magnesium By similarity
Metal bindingi409 – 4091Magnesium By similarity
Metal bindingi445 – 4451Iron-sulfur (4Fe-4S) By similarity
Metal bindingi501 – 5011Iron-sulfur (4Fe-4S) By similarity
Metal bindingi504 – 5041Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:ML2206
ORF Names:MLCB5.38
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML2206.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5757 By similarity
PRO_0000029255Add
BLAST
Chaini58 – 556499Amidophosphoribosyltransferase
PRO_0000029256Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML2206.

Structurei

3D structure databases

ProteinModelPortaliQ50028.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 284227Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiSGIPYEM.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q50028-1 [UniParc]FASTAAdd to Basket

« Hide

MCLAVGVGVR APKHVPQIRR LGRAGRRLRC VTNCALGSCP IVTVQQPGRD    50
FSSPREECGV FGVWAPGELV AKLTYFGLYA LQHRGQEAAG IAVADGSQVL 100
VFKDLGLVSQ VFDEQTLAAM EGHVAIGHCR YSTTGDTTWE NAQPVFRNIA 150
AGSGVALGHN GNLVNTAELA ARARDAGLIA KRCPAPATTD SDILGALLAH 200
GAADSTLEQA ALELLPTVRG AFCLTFMDEN TLYACRDPYG VRPLSLGRLD 250
RGWVVASETA GLDIVGASFV RDIEPGELLA IDADGVRSTR FANPTPKGCV 300
FEYVYLARPD STLAGRSVHG TRVEIGRRLA RECPVEADLV IGVPESGTPA 350
AVGYAQESGI SYGQGLMKNA YVGRTFIQPS QTIRQLGIRL KLNPLKEVIR 400
GKRLIVVDDS VVRGNTQRAL VRMLREAGAV ELHVRIASPP VKWPCFYGID 450
FPSPAELIAN VVADEEEMLE AVRQGIGADT LGYISLRGMI AASEQPASRL 500
CYACFDGRYP IELPSEAMLG KNVIEHMLAN AARGAGLRDL AADQVPVDAD 550
ENCVWR 556
Length:556
Mass (Da):59,540
Last modified:November 1, 1996 - v1
Checksum:iE0CF4D278302FDCE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15182 Genomic DNA. Translation: AAA62988.1.
Z95151 Genomic DNA. Translation: CAB08437.1.
AL583924 Genomic DNA. Translation: CAC31161.1.
PIRiA87185.
RefSeqiNP_302447.1. NC_002677.1.
WP_010908767.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31161; CAC31161; CAC31161.
GeneIDi908827.
KEGGimle:ML2206.
PATRICi18058519. VBIMycLep78757_4178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15182 Genomic DNA. Translation: AAA62988.1 .
Z95151 Genomic DNA. Translation: CAB08437.1 .
AL583924 Genomic DNA. Translation: CAC31161.1 .
PIRi A87185.
RefSeqi NP_302447.1. NC_002677.1.
WP_010908767.1. NC_002677.1.

3D structure databases

ProteinModelPortali Q50028.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272631.ML2206.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC31161 ; CAC31161 ; CAC31161 .
GeneIDi 908827.
KEGGi mle:ML2206.
PATRICi 18058519. VBIMycLep78757_4178.

Organism-specific databases

Lepromai ML2206.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
KOi K00764.
OMAi SGIPYEM.
OrthoDBi EOG6KT2Q1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPUR1_MYCLE
AccessioniPrimary (citable) accession number: Q50028
Secondary accession number(s): O05762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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