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Q50028

- PUR1_MYCLE

UniProt

Q50028 - PUR1_MYCLE

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei58 – 581For GATase activityBy similarity
    Metal bindingi299 – 2991Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi346 – 3461MagnesiumBy similarity
    Metal bindingi408 – 4081MagnesiumBy similarity
    Metal bindingi409 – 4091MagnesiumBy similarity
    Metal bindingi445 – 4451Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi501 – 5011Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi504 – 5041Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:ML2206
    ORF Names:MLCB5.38
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML2206.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 5757By similarityPRO_0000029255Add
    BLAST
    Chaini58 – 556499AmidophosphoribosyltransferasePRO_0000029256Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272631.ML2206.

    Structurei

    3D structure databases

    ProteinModelPortaliQ50028.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 284227Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    KOiK00764.
    OMAiSGIPYEM.
    OrthoDBiEOG6KT2Q1.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q50028-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCLAVGVGVR APKHVPQIRR LGRAGRRLRC VTNCALGSCP IVTVQQPGRD    50
    FSSPREECGV FGVWAPGELV AKLTYFGLYA LQHRGQEAAG IAVADGSQVL 100
    VFKDLGLVSQ VFDEQTLAAM EGHVAIGHCR YSTTGDTTWE NAQPVFRNIA 150
    AGSGVALGHN GNLVNTAELA ARARDAGLIA KRCPAPATTD SDILGALLAH 200
    GAADSTLEQA ALELLPTVRG AFCLTFMDEN TLYACRDPYG VRPLSLGRLD 250
    RGWVVASETA GLDIVGASFV RDIEPGELLA IDADGVRSTR FANPTPKGCV 300
    FEYVYLARPD STLAGRSVHG TRVEIGRRLA RECPVEADLV IGVPESGTPA 350
    AVGYAQESGI SYGQGLMKNA YVGRTFIQPS QTIRQLGIRL KLNPLKEVIR 400
    GKRLIVVDDS VVRGNTQRAL VRMLREAGAV ELHVRIASPP VKWPCFYGID 450
    FPSPAELIAN VVADEEEMLE AVRQGIGADT LGYISLRGMI AASEQPASRL 500
    CYACFDGRYP IELPSEAMLG KNVIEHMLAN AARGAGLRDL AADQVPVDAD 550
    ENCVWR 556
    Length:556
    Mass (Da):59,540
    Last modified:November 1, 1996 - v1
    Checksum:iE0CF4D278302FDCE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15182 Genomic DNA. Translation: AAA62988.1.
    Z95151 Genomic DNA. Translation: CAB08437.1.
    AL583924 Genomic DNA. Translation: CAC31161.1.
    PIRiA87185.
    RefSeqiNP_302447.1. NC_002677.1.
    WP_010908767.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC31161; CAC31161; CAC31161.
    GeneIDi908827.
    KEGGimle:ML2206.
    PATRICi18058519. VBIMycLep78757_4178.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15182 Genomic DNA. Translation: AAA62988.1 .
    Z95151 Genomic DNA. Translation: CAB08437.1 .
    AL583924 Genomic DNA. Translation: CAC31161.1 .
    PIRi A87185.
    RefSeqi NP_302447.1. NC_002677.1.
    WP_010908767.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali Q50028.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML2206.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC31161 ; CAC31161 ; CAC31161 .
    GeneIDi 908827.
    KEGGi mle:ML2206.
    PATRICi 18058519. VBIMycLep78757_4178.

    Organism-specific databases

    Lepromai ML2206.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    KOi K00764.
    OMAi SGIPYEM.
    OrthoDBi EOG6KT2Q1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Smith D.R., Robison K.
      Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiPUR1_MYCLE
    AccessioniPrimary (citable) accession number: Q50028
    Secondary accession number(s): O05762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3