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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581NucleophileUniRule annotation
Metal bindingi299 – 2991Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi346 – 3461MagnesiumUniRule annotation
Metal bindingi408 – 4081MagnesiumUniRule annotation
Metal bindingi409 – 4091MagnesiumUniRule annotation
Metal bindingi445 – 4451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi501 – 5011Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi504 – 5041Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:ML2206
ORF Names:MLCB5.38
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML2206.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5757By similarityPRO_0000029255Add
BLAST
Chaini58 – 556499AmidophosphoribosyltransferasePRO_0000029256Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML2206.

Structurei

3D structure databases

ProteinModelPortaliQ50028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 284227Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q50028-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCLAVGVGVR APKHVPQIRR LGRAGRRLRC VTNCALGSCP IVTVQQPGRD
60 70 80 90 100
FSSPREECGV FGVWAPGELV AKLTYFGLYA LQHRGQEAAG IAVADGSQVL
110 120 130 140 150
VFKDLGLVSQ VFDEQTLAAM EGHVAIGHCR YSTTGDTTWE NAQPVFRNIA
160 170 180 190 200
AGSGVALGHN GNLVNTAELA ARARDAGLIA KRCPAPATTD SDILGALLAH
210 220 230 240 250
GAADSTLEQA ALELLPTVRG AFCLTFMDEN TLYACRDPYG VRPLSLGRLD
260 270 280 290 300
RGWVVASETA GLDIVGASFV RDIEPGELLA IDADGVRSTR FANPTPKGCV
310 320 330 340 350
FEYVYLARPD STLAGRSVHG TRVEIGRRLA RECPVEADLV IGVPESGTPA
360 370 380 390 400
AVGYAQESGI SYGQGLMKNA YVGRTFIQPS QTIRQLGIRL KLNPLKEVIR
410 420 430 440 450
GKRLIVVDDS VVRGNTQRAL VRMLREAGAV ELHVRIASPP VKWPCFYGID
460 470 480 490 500
FPSPAELIAN VVADEEEMLE AVRQGIGADT LGYISLRGMI AASEQPASRL
510 520 530 540 550
CYACFDGRYP IELPSEAMLG KNVIEHMLAN AARGAGLRDL AADQVPVDAD

ENCVWR
Length:556
Mass (Da):59,540
Last modified:November 1, 1996 - v1
Checksum:iE0CF4D278302FDCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15182 Genomic DNA. Translation: AAA62988.1.
Z95151 Genomic DNA. Translation: CAB08437.1.
AL583924 Genomic DNA. Translation: CAC31161.1.
PIRiA87185.
RefSeqiNP_302447.1. NC_002677.1.
WP_010908767.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31161; CAC31161; CAC31161.
GeneIDi908827.
KEGGimle:ML2206.
PATRICi18058519. VBIMycLep78757_4178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15182 Genomic DNA. Translation: AAA62988.1.
Z95151 Genomic DNA. Translation: CAB08437.1.
AL583924 Genomic DNA. Translation: CAC31161.1.
PIRiA87185.
RefSeqiNP_302447.1. NC_002677.1.
WP_010908767.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ50028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2206.

Protein family/group databases

MEROPSiC44.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31161; CAC31161; CAC31161.
GeneIDi908827.
KEGGimle:ML2206.
PATRICi18058519. VBIMycLep78757_4178.

Organism-specific databases

LepromaiML2206.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPUR1_MYCLE
AccessioniPrimary (citable) accession number: Q50028
Secondary accession number(s): O05762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.