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Q50028 (PUR1_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:ML2206
ORF Names:MLCB5.38
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 5757 By similarity
PRO_0000029255
Chain58 – 556499Amidophosphoribosyltransferase
PRO_0000029256

Regions

Domain58 – 284227Glutamine amidotransferase type-2

Sites

Active site581For GATase activity By similarity
Metal binding2991Iron-sulfur (4Fe-4S) By similarity
Metal binding3461Magnesium By similarity
Metal binding4081Magnesium By similarity
Metal binding4091Magnesium By similarity
Metal binding4451Iron-sulfur (4Fe-4S) By similarity
Metal binding5011Iron-sulfur (4Fe-4S) By similarity
Metal binding5041Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q50028 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E0CF4D278302FDCE

FASTA55659,540
        10         20         30         40         50         60 
MCLAVGVGVR APKHVPQIRR LGRAGRRLRC VTNCALGSCP IVTVQQPGRD FSSPREECGV 

        70         80         90        100        110        120 
FGVWAPGELV AKLTYFGLYA LQHRGQEAAG IAVADGSQVL VFKDLGLVSQ VFDEQTLAAM 

       130        140        150        160        170        180 
EGHVAIGHCR YSTTGDTTWE NAQPVFRNIA AGSGVALGHN GNLVNTAELA ARARDAGLIA 

       190        200        210        220        230        240 
KRCPAPATTD SDILGALLAH GAADSTLEQA ALELLPTVRG AFCLTFMDEN TLYACRDPYG 

       250        260        270        280        290        300 
VRPLSLGRLD RGWVVASETA GLDIVGASFV RDIEPGELLA IDADGVRSTR FANPTPKGCV 

       310        320        330        340        350        360 
FEYVYLARPD STLAGRSVHG TRVEIGRRLA RECPVEADLV IGVPESGTPA AVGYAQESGI 

       370        380        390        400        410        420 
SYGQGLMKNA YVGRTFIQPS QTIRQLGIRL KLNPLKEVIR GKRLIVVDDS VVRGNTQRAL 

       430        440        450        460        470        480 
VRMLREAGAV ELHVRIASPP VKWPCFYGID FPSPAELIAN VVADEEEMLE AVRQGIGADT 

       490        500        510        520        530        540 
LGYISLRGMI AASEQPASRL CYACFDGRYP IELPSEAMLG KNVIEHMLAN AARGAGLRDL 

       550 
AADQVPVDAD ENCVWR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15182 Genomic DNA. Translation: AAA62988.1.
Z95151 Genomic DNA. Translation: CAB08437.1.
AL583924 Genomic DNA. Translation: CAC31161.1.
PIRA87185.
RefSeqNP_302447.1. NC_002677.1.

3D structure databases

ProteinModelPortalQ50028.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML2206.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31161; CAC31161; CAC31161.
GeneID908827.
KEGGmle:ML2206.
PATRIC18058519. VBIMycLep78757_4178.

Organism-specific databases

LepromaML2206.
CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMASGIPYEM.
OrthoDBEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_MYCLE
AccessionPrimary (citable) accession number: Q50028
Secondary accession number(s): O05762
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways