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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.By similarity

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei393 – 3931FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADBy similarity
Nucleotide bindingi36 – 372FADBy similarity
Nucleotide bindingi58 – 614FADBy similarity
Nucleotide bindingi429 – 4313FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
Ordered Locus Names:ML1044
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1044.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Protoporphyrinogen oxidasePRO_0000135264Add
BLAST

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi272631.ML1044.

Structurei

3D structure databases

ProteinModelPortaliQ50008.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiCOG1232.
HOGENOMiHOG000269479.
KOiK00231.
OMAiFTTKWGH.
OrthoDBiEOG6RFZTN.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

Q50008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRSYCVVG GGISGLTAAY RLRVATGDDV AITLFDPGDR LGGVLRTECV
60 70 80 90 100
GGQPMDLGAE AFLLRRPEVP ALLAELGLSE RQRATTDARP LIYSQQRLHS
110 120 130 140 150
LPPDTVAGIP SSATSVAGLV DDATVARIGA EAVRPLSWEP GSDPAMAELV
160 170 180 190 200
ADRFGEQAVA RLVDPLLGGV YAGSAATIGL RAGAPSVAAA LDCGATSLME
210 220 230 240 250
AVRQGLPPVA AGPVFGALDG GYQVLIDELV RRSRLQWVAA TVVGLDRGTC
260 270 280 290 300
GWTLVDDTGA CWSADGVILA VPAPRLVRLL QQIAPRTVAA ASRIVSASSA
310 320 330 340 350
VVALSVPRDT TFPQNSGVLV ASGERLRAKA VTLSSRKWGL QGDTQLVRLS
360 370 380 390 400
FGKFGDQVAS TASDDELLAW AVSDLAAVFD VTVDPVDVCV QRWIDAMPQY
410 420 430 440 450
GPGHADLVAE VRAGLPPTLV VAGSHMDGIG VPACISAAGR AIEALQAEVA

R
Length:451
Mass (Da):46,580
Last modified:October 31, 1996 - v1
Checksum:iDF76EE1655CA2056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15181 Genomic DNA. Translation: AAA62958.1.
AL583920 Genomic DNA. Translation: CAC31425.1.
PIRiF87039.
RefSeqiNP_301770.1. NC_002677.1.
WP_010908094.1. NC_002677.1.

Genome annotation databases

GeneIDi910131.
KEGGimle:ML1044.
PATRICi18053862. VBIMycLep78757_1876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15181 Genomic DNA. Translation: AAA62958.1.
AL583920 Genomic DNA. Translation: CAC31425.1.
PIRiF87039.
RefSeqiNP_301770.1. NC_002677.1.
WP_010908094.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ50008.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi910131.
KEGGimle:ML1044.
PATRICi18053862. VBIMycLep78757_1876.

Organism-specific databases

LepromaiML1044.

Phylogenomic databases

eggNOGiCOG1232.
HOGENOMiHOG000269479.
KOiK00231.
OMAiFTTKWGH.
OrthoDBiEOG6RFZTN.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPPOX_MYCLE
AccessioniPrimary (citable) accession number: Q50008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.