Reviewed, UniProtKB/Swiss-Prot Q4ZYD3 (CHEB1_PSEU2)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Psyr_0781

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225476
Regions
	Domain	7 – 124	118	Response regulatory
	Domain	170 – 358	189	CheB-type methylesterase
Sites
	Active site	182	1	By similarity
	Active site	208	1	By similarity
	Active site	304	1	By similarity
Amino acid modifications
	Modified residue	58	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZYD3-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 1DFDE66425F31A93	FASTA	358	37,997
	10 20 30 40 50 60 MPNKKISVLL VDDSAVVRQV LVAILNDTPD IHVMGAASDP IFAMSKLAQE WPDVIVLDVE 70 80 90 100 110 120 MPRMDGITFL KKIMSERPTP VVICSSLTQK GAETSLQALS AGAVEIITKP TTGLKNFLID 130 140 150 160 170 180 SAAELVAAVR AAANSNVRNL GKRTAAPVLN PASKLTADAI LPAASGHAMA QTTERIVAIG 190 200 210 220 230 240 TSTGGTQALE AVLTALPRVC PGMVIVQHMP EKFTASFAER LNSLSQIEVR EARNNDRILP 250 260 270 280 290 300 GLALIAPGGK HMMVTRSGAY YHVQVIDGPL VNRHRPSVDV LFRSVAKFAG KNATGIIMTG 310 320 330 340 350 MGDDGARGLK EMLDAGSATV AQDEASCVVF GMPKEAIKLN AAQRIIPLQE IHQAILHR

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000075 Genomic DNA. Translation: AAY35839.1.
RefSeq	YP_233877.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZYD3.
Genome annotation databases
GeneID	3366268.
GenomeReviews	Gene locus Psyr_0781 in contig CP000075_GR.
KEGG	psb:Psyr_0781.
NMPDR	fig|205918.4.peg.1102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4ZYD3.
OMA	VMCSSLT.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_0781-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_PSEU2

Accession

Primary (citable) accession number: Q4ZYD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)