ID   CAPP_PSEU2              Reviewed;         878 AA.
AC   Q4ZWV3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   27-MAR-2024, entry version 94.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595};
GN   OrderedLocusNames=Psyr_1318;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CP000075; AAY36369.1; -; Genomic_DNA.
DR   RefSeq; WP_011266958.1; NC_007005.1.
DR   RefSeq; YP_234407.1; NC_007005.1.
DR   AlphaFoldDB; Q4ZWV3; -.
DR   SMR; Q4ZWV3; -.
DR   STRING; 205918.Psyr_1318; -.
DR   KEGG; psb:Psyr_1318; -.
DR   PATRIC; fig|205918.7.peg.1351; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium.
FT   CHAIN           1..878
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_1000025581"
FT   ACT_SITE        140
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        545
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   878 AA;  97595 MW;  EA7EC59CBEE69C0D CRC64;
     MADIDARLRE DVHLLGELLG NTIREQRGAE FLDKIERIRK GAKAGRRGSA EGAEQLSSSV
     DGLGDDELLP VARAFNQFLN LANIAEQYQL MHRRDDKQPL PFESRVLPEL LDRLKAEGHS
     PDALARQLSK LEIDLVLTAH PTEVARRTLI QKYDAIAAQL AALDHRDLNS IERTQITSRL
     QRLIAEAWHT EEIRRIRPTP VDEAKWGFAV IEHSLWHAIP NYLRKADHAL HAATGLHLPL
     EAAPIRFASW MGGDRDGNPN VTAAVTREVL LLARWMAADL YLRDVDNLAA ELSMQQASDA
     LRASVGDSAE PYRAELKRLR ERLRATRNWA NASLSETLPA PEAVLRDNRE LLDPLLLCFQ
     SLHECGMGVI ADGPLLDCLR RAVTFGLFLV RLDVRQDSSR HCAAMTEITD YLGLGRYEEW
     DEQTRIDFLL RELNNRRPLL PSYFKPAADT AEVLATCREV AAAPAASLGS YVISMAGSAS
     DVLAVQLLLK ESGLQRPMRV VPLFETLADL DNAGPVIETL LGLPGYRSRL HGPQEVMIGY
     SDSAKDAGTT AAAWAQYRAQ ERLVEICREQ QVELLLFHGR GGTVGRGGGP AHAAILSQPP
     GSVAGRFRTT EQGEMIRFKF GLPDIAEQNL NLYLAAVLEA TLLPPPPPQP AWRTMMDQMA
     DDGVSAYRAV VRENPEFVEY FRQATPEQEL GRLPLGSRPA KRREGGVESL RAIPWIFAWT
     QTRLMLPAWL GWEAALSKAL ERGEGEVLAQ MREQWPFFRT RIDMLEMVLA KADADIARLY
     DERLVSAELQ HLGAHLRDLL SQACNVVLGL TGQTQLLAHS PETLEFISLR NTYLDPLHLL
     QAELLSRSRN REASLDSPLE LALLVSVAGI AAGLRNTG
//