Q4ZW63 (MAO1_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38

Gene names

Name:	maeA
Ordered Locus Names:	Psyr_1561

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.
Sequence caution	The sequence AAY36609.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160226
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	416	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZW63 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: C4D76794E57CB6EA	FASTA	563	62,285
	10 20 30 40 50 60 MTKTSRPLYI SYAGPSLLEM PLLNKGSAFT PQERVEFNLI GLLPQNVETI EEQVTRVYSQ 70 80 90 100 110 120 YKQCASDLDK HIYLRSIQDN NETLFFRLLD SHLDEMLPII YTPTVGQACQ EFSKIYRTHR 130 140 150 160 170 180 GLFISYPERD RIDDILRSAT KDRIKIIVVT DSERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPI VLDVGTNNRE LLDDPMYMGW RHERVSGKEY EDFIALFIDA VQRRWPDVLL 250 260 270 280 290 300 QFEDFAQSNA MPLLEKYRDE LCCFNDDIQG TASVAVGTLL AACKAKNETL GQQKVVFVGA 310 320 330 340 350 360 GSAGCGIAEH IIAAMRIEGL SESEARKRIF MVDRFGLLTE SMDNLLDFQK RLAQKTADVS 370 380 390 400 410 420 GWTAGSEAFP QLLDVVTHAG ATVMIGVSGQ RGLFTEQVIR ELHKHCAKPL VMPLSNPTSK 430 440 450 460 470 480 VEATPEEILR WTDGNALVAT GSPFAPVEIN GRTVHIAQCN NSYIFPGIGL GVVACKASRI 490 500 510 520 530 540 TDRMLMAASN ALAECSPMVT GKGDAVLPPL KEIQQVSRKI ALAVAREAQA EGLALETTEE 550 560 ALLEAIERNF WLPGYRAYRR RSV

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B728a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY36609.1. Different initiation.
RefSeq	YP_234647.2. NC_007005.1.
3D structure databases
ProteinModelPortal	Q4ZW63.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZW63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3367061.
GenomeReviews	Gene locus Psyr_1561 in contig CP000075_GR.
KEGG	psb:Psyr_1561.
NMPDR	fig|205918.4.peg.2043.
PATRIC	19983625. VBIPseSyr42314_1595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
ProtClustDB	PRK13529.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_1561-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSEU2

Accession

Primary (citable) accession number: Q4ZW63

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program