Q4ZV77 (STHA_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	Psyr_1901

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Sequence caution	The sequence AAY36945.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000260241
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZV77 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 21CFE19287233998	FASTA	464	50,783
	10 20 30 40 50 60 MAVYNYDVVV LGSGPAGEGA AMNAAKAGRK VAMVDSRRQV GGNCTHLGTI PSKALRHSVK 70 80 90 100 110 120 QIIQFNTNPM FRAIGEPRWF SFPDVLKNAE MVISKQVASR TSYYARNRVD VFFGTGSFAD 130 140 150 160 170 180 ETSINVVCTN GVVEKLVANQ IIIATGSRPY RPADIDFSHK RIYDSDTILS LGHTPRKLII 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VELVDNRDQL LSFLDSEISQ ALSYHFSNNN VMVRHNEEYE 250 260 270 280 290 300 KVEGLDNGVI LHLKSGKKIK ADALLWCNGR TGNTDKLGLE NIGLKANGRG QIEVDETYRT 310 320 330 340 350 360 SVSNVYGAGD VIGWPSLASA AYDQGRSAAG SMVDNGSWRY VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 EHELTQAKVP YEVGKAFFKG MARAQISGER VGMLKILFHR ETLEVLGVHC FGDQASEIVH 430 440 450 460 IGQAIMSQPG EANTMKYFVN TTFNYPTMAE AYRVAAYDGL NRLF

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B728a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY36945.1. Different initiation.
RefSeq	YP_234983.1. NC_007005.1.
3D structure databases
HSSP	HSSP built from PDB template 2F5Z based on UniProtKB P09622.
ProteinModelPortal	Q4ZV77.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZV77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3367411.
GenomeReviews	Gene locus Psyr_1901 in contig CP000075_GR.
KEGG	psb:Psyr_1901.
NMPDR	fig|205918.4.peg.2379.
PATRIC	19984343. VBIPseSyr42314_1945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HBG515043.
ProtClustDB	PRK05249.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_1901-MONOMER.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00322.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_PSEU2

Accession

Primary (citable) accession number: Q4ZV77

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program