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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (Psyr_1148)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi352Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi413Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi416Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPSYR205918:G1G4J-2022-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:Psyr_1983
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
Proteomesi
  • UP000000426 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000767881 – 4743-isopropylmalate dehydratase large subunitAdd BLAST474

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi205918.Psyr_1983

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI Bacteria
COG0065 LUCA
HOGENOMiHOG000226972
KOiK01703
OMAiFDHQVPA
OrthoDBiPOG091H02GD

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01026 LeuC_type1, 1 hit
InterProiView protein in InterPro
IPR004430 3-IsopropMal_deHydase_lsu
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR00170 leuC, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q4ZUZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKTLYDKL WDSHLVKQRD DGSALIYIDR HIIHEVTSPQ AFEGLRLAKR
60 70 80 90 100
KPWRIDSIIA TPDHNVPTTA ERKGGIGAIE DQVSRLQVQT LDDNCDEYGI
110 120 130 140 150
TEFKMNDPRQ GIVHVIGPEQ GATLPGMSVV CGDSHTSTHG AFGALAHGIG
160 170 180 190 200
TSEVEHVLAT QCLVAKKMKN MLVSVEGQLP FGVTAKDIVL AVIGKIGTAG
210 220 230 240 250
GNGYAIEFAG SAIRDLSIEG RMTICNMSIE AGARVGMVAT DEKTVEYVKG
260 270 280 290 300
RPFAPKGAEW DLAVEAWKDL VSDPDAVFDT VVRLDAAQIK PQVSWGTSPE
310 320 330 340 350
MVLAVDQNVP DPAQEPDLVK RGSIERALKY MGLKANQPIT DIQLDRVFIG
360 370 380 390 400
SCTNSRIEDL RAAADVAKGR KVASTIKQAI VVPGSGLIKA QAEKEGLDKV
410 420 430 440 450
FIEAGFEWRE PGCSMCLAMN PDRLGSGEHC ASTSNRNFEG RQGAGGRTHL
460 470
VSPAMAAAAA VNGRFIDVRD LIQH
Length:474
Mass (Da):50,968
Last modified:June 7, 2005 - v1
Checksum:iFFC844498821359A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000075 Genomic DNA Translation: AAY37026.1
RefSeqiWP_003406190.1, NC_007005.1
YP_235064.1, NC_007005.1

Genome annotation databases

EnsemblBacteriaiAAY37026; AAY37026; Psyr_1983
GeneIDi3367497
KEGGipsb:Psyr_1983
PATRICifig|205918.7.peg.2026

Similar proteinsi

Entry informationi

Entry nameiLEUC_PSEU2
AccessioniPrimary (citable) accession number: Q4ZUZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health