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Q4ZUZ6 (LEUC_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuC
Ordered Locus Names:Psyr_1983
OrganismPseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]
Taxonomic identifier205918 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4744743-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
PRO_0000076788

Sites

Metal binding3521Iron-sulfur (4Fe-4S) By similarity
Metal binding4131Iron-sulfur (4Fe-4S) By similarity
Metal binding4161Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ZUZ6 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: FFC844498821359A

FASTA47450,968
        10         20         30         40         50         60 
MAGKTLYDKL WDSHLVKQRD DGSALIYIDR HIIHEVTSPQ AFEGLRLAKR KPWRIDSIIA 

        70         80         90        100        110        120 
TPDHNVPTTA ERKGGIGAIE DQVSRLQVQT LDDNCDEYGI TEFKMNDPRQ GIVHVIGPEQ 

       130        140        150        160        170        180 
GATLPGMSVV CGDSHTSTHG AFGALAHGIG TSEVEHVLAT QCLVAKKMKN MLVSVEGQLP 

       190        200        210        220        230        240 
FGVTAKDIVL AVIGKIGTAG GNGYAIEFAG SAIRDLSIEG RMTICNMSIE AGARVGMVAT 

       250        260        270        280        290        300 
DEKTVEYVKG RPFAPKGAEW DLAVEAWKDL VSDPDAVFDT VVRLDAAQIK PQVSWGTSPE 

       310        320        330        340        350        360 
MVLAVDQNVP DPAQEPDLVK RGSIERALKY MGLKANQPIT DIQLDRVFIG SCTNSRIEDL 

       370        380        390        400        410        420 
RAAADVAKGR KVASTIKQAI VVPGSGLIKA QAEKEGLDKV FIEAGFEWRE PGCSMCLAMN 

       430        440        450        460        470 
PDRLGSGEHC ASTSNRNFEG RQGAGGRTHL VSPAMAAAAA VNGRFIDVRD LIQH 

« Hide

References

[1]"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000."
Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E.
Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B728a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000075 Genomic DNA. Translation: AAY37026.1.
RefSeqYP_235064.1. NC_007005.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205918.Psyr_1983.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY37026; AAY37026; Psyr_1983.
GeneID3367497.
KEGGpsb:Psyr_1983.
PATRIC19984513. VBIPseSyr42314_2026.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMADKVWDLH.
OrthoDBEOG600DP5.
ProtClustDBPRK05478.

Enzyme and pathway databases

BioCycPSYR205918:GJ94-2022-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEUC_PSEU2
AccessionPrimary (citable) accession number: Q4ZUZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 7, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways