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Q4ZUZ6

- LEUC_PSEU2

UniProt

Q4ZUZ6 - LEUC_PSEU2

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi352 – 3521Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi413 – 4131Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi416 – 4161Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciPSYR205918:GJ94-2022-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:Psyr_1983
    OrganismiPseudomonas syringae pv. syringae (strain B728a)
    Taxonomic identifieri205918 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
    ProteomesiUP000000426: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4744743-isopropylmalate dehydratase large subunitPRO_0000076788Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi205918.Psyr_1983.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiPASHREY.
    OrthoDBiEOG600DP5.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4ZUZ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKTLYDKL WDSHLVKQRD DGSALIYIDR HIIHEVTSPQ AFEGLRLAKR    50
    KPWRIDSIIA TPDHNVPTTA ERKGGIGAIE DQVSRLQVQT LDDNCDEYGI 100
    TEFKMNDPRQ GIVHVIGPEQ GATLPGMSVV CGDSHTSTHG AFGALAHGIG 150
    TSEVEHVLAT QCLVAKKMKN MLVSVEGQLP FGVTAKDIVL AVIGKIGTAG 200
    GNGYAIEFAG SAIRDLSIEG RMTICNMSIE AGARVGMVAT DEKTVEYVKG 250
    RPFAPKGAEW DLAVEAWKDL VSDPDAVFDT VVRLDAAQIK PQVSWGTSPE 300
    MVLAVDQNVP DPAQEPDLVK RGSIERALKY MGLKANQPIT DIQLDRVFIG 350
    SCTNSRIEDL RAAADVAKGR KVASTIKQAI VVPGSGLIKA QAEKEGLDKV 400
    FIEAGFEWRE PGCSMCLAMN PDRLGSGEHC ASTSNRNFEG RQGAGGRTHL 450
    VSPAMAAAAA VNGRFIDVRD LIQH 474
    Length:474
    Mass (Da):50,968
    Last modified:June 7, 2005 - v1
    Checksum:iFFC844498821359A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000075 Genomic DNA. Translation: AAY37026.1.
    RefSeqiYP_235064.1. NC_007005.1.

    Genome annotation databases

    EnsemblBacteriaiAAY37026; AAY37026; Psyr_1983.
    GeneIDi3367497.
    KEGGipsb:Psyr_1983.
    PATRICi19984513. VBIPseSyr42314_2026.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000075 Genomic DNA. Translation: AAY37026.1 .
    RefSeqi YP_235064.1. NC_007005.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205918.Psyr_1983.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY37026 ; AAY37026 ; Psyr_1983 .
    GeneIDi 3367497.
    KEGGi psb:Psyr_1983.
    PATRICi 19984513. VBIPseSyr42314_2026.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi PASHREY.
    OrthoDBi EOG600DP5.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci PSYR205918:GJ94-2022-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B728a.

    Entry informationi

    Entry nameiLEUC_PSEU2
    AccessioniPrimary (citable) accession number: Q4ZUZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3