Q4ZUZ6 (LEUC_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase large subunit
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase

Gene names

Name:	leuC
Ordered Locus Names:	Psyr_1983

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 1 › Pseudomonas syringae › Pseudomonas syringae pv. syringae

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026
Subunit structure	Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026
Sequence similarities	Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	3-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026		PRO_0000076788
Sites
	Metal binding	352	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	413	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	416	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZUZ6 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: FFC844498821359A	FASTA	474	50,968
	10 20 30 40 50 60 MAGKTLYDKL WDSHLVKQRD DGSALIYIDR HIIHEVTSPQ AFEGLRLAKR KPWRIDSIIA 70 80 90 100 110 120 TPDHNVPTTA ERKGGIGAIE DQVSRLQVQT LDDNCDEYGI TEFKMNDPRQ GIVHVIGPEQ 130 140 150 160 170 180 GATLPGMSVV CGDSHTSTHG AFGALAHGIG TSEVEHVLAT QCLVAKKMKN MLVSVEGQLP 190 200 210 220 230 240 FGVTAKDIVL AVIGKIGTAG GNGYAIEFAG SAIRDLSIEG RMTICNMSIE AGARVGMVAT 250 260 270 280 290 300 DEKTVEYVKG RPFAPKGAEW DLAVEAWKDL VSDPDAVFDT VVRLDAAQIK PQVSWGTSPE 310 320 330 340 350 360 MVLAVDQNVP DPAQEPDLVK RGSIERALKY MGLKANQPIT DIQLDRVFIG SCTNSRIEDL 370 380 390 400 410 420 RAAADVAKGR KVASTIKQAI VVPGSGLIKA QAEKEGLDKV FIEAGFEWRE PGCSMCLAMN 430 440 450 460 470 PDRLGSGEHC ASTSNRNFEG RQGAGGRTHL VSPAMAAAAA VNGRFIDVRD LIQH

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B728a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY37026.1.
RefSeq	YP_235064.1. NC_007005.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	205918.Psyr_1983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY37026; AAY37026; Psyr_1983.
GeneID	3367497.
KEGG	psb:Psyr_1983.
PATRIC	19984513. VBIPseSyr42314_2026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0065.
HOGENOM	HOG000226972.
KO	K01703.
OMA	DIRQGIV.
ProtClustDB	PRK05478.
Enzyme and pathway databases
BioCyc	PSYR205918:GJ94-2022-MONOMER.
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.30.499.10. 2 hits. 3.40.1060.10. 1 hit.
HAMAP	MF_01026. LeuC_type1.
InterPro	IPR004430. 3-IsopropMal_deHydase_lsu. IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR00170. leuC. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEUC_PSEU2

Accession

Primary (citable) accession number: Q4ZUZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program