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Q4ZTJ2

- GLGB_PSEU2

UniProt

Q4ZTJ2 - GLGB_PSEU2

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei420 – 4201NucleophileUniRule annotation
Active sitei473 – 4731Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciPSYR205918:GJ94-2533-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Psyr_2491
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
ProteomesiUP000000426: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7417411,4-alpha-glucan branching enzyme GlgBPRO_0000260680Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi205918.Psyr_2491.

Structurei

3D structure databases

ProteinModelPortaliQ4ZTJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4ZTJ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNAPDKTGTR RRAIPAAVDL DALIRAEHRD PFSILGPHGD GGSGQYVRAY
60 70 80 90 100
LPAALSVRLL ARDDGRELGE MEMSEVPGFF VGHLEHPQPY LLKINWAGGE
110 120 130 140 150
QITEDPYSFG PLLGEMDLYL FAEGNHRDLS SCLGAQVTSV DGVDGVRFAV
160 170 180 190 200
WAPNARRVSV VGSFNSWDGR RHPMRMRHPT GVWEIFVPRL QPGEVYKYEI
210 220 230 240 250
LGAHGILPLK SDPMALSTTL PPDTASKIAA PLQFEWNDQE WLQSRAGRHE
260 270 280 290 300
VTAPLSIYEL HAGSWQMEQV EDNQWRQYNW RELADRLIPY VKELGFTHIE
310 320 330 340 350
LMPIMEHPFG GSWGYQLLAQ FAPTARYGSP EDFAFFVDAC HRAEIGVILD
360 370 380 390 400
WVPAHFPTDT HGLAQFDGTC LYEYADPKEG FHQDWNTLIY NLGRTEVHGF
410 420 430 440 450
MLASALHWLK HYHIDGLRVD AVASMLYRDY SRNAGEWVTN RFGGRENLEA
460 470 480 490 500
IDFLRHLNDV VALEAPGTMV IAEESTAWPG VSEPTQQGGL GFNYKWNMGW
510 520 530 540 550
MHDSLQYMEE DPINRGHHHG KLSFSLVYAW SERFVLPISH DEVVHGKHSL
560 570 580 590 600
IDKMPGDRWQ KFANLRAYLS FMWTHPGKKL LFMGCEFGQW REWNHDRELD
610 620 630 640 650
WYLMQYAEHV GVKNLVGDLN RLYREEKALH ERDADPAGFQ WLVGDDSANS
660 670 680 690 700
VFAYLRWSND GEPLLVVANM TPVPRLDYRL GAPMRGAWTE LLNSDAETYA
710 720 730 740
GSNFGNGGEV MTEAEPAHGM EDSLVLNLPP LAVLILKPKK D
Length:741
Mass (Da):84,062
Last modified:June 7, 2005 - v1
Checksum:i30571708F8CEEF0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY37530.1.
RefSeqiWP_011267721.1. NC_007005.1.
YP_235568.1. NC_007005.1.

Genome annotation databases

EnsemblBacteriaiAAY37530; AAY37530; Psyr_2491.
GeneIDi3368008.
KEGGipsb:Psyr_2491.
PATRICi19985570. VBIPseSyr42314_2551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY37530.1 .
RefSeqi WP_011267721.1. NC_007005.1.
YP_235568.1. NC_007005.1.

3D structure databases

ProteinModelPortali Q4ZTJ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205918.Psyr_2491.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY37530 ; AAY37530 ; Psyr_2491 .
GeneIDi 3368008.
KEGGi psb:Psyr_2491.
PATRICi 19985570. VBIPseSyr42314_2551.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci PSYR205918:GJ94-2533-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B728a.

Entry informationi

Entry nameiGLGB_PSEU2
AccessioniPrimary (citable) accession number: Q4ZTJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 7, 2005
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3