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Q4ZTJ2 (GLGB_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Psyr_2491
OrganismPseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]
Taxonomic identifier205918 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7417411,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260680

Sites

Active site4201Nucleophile By similarity
Active site4731Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ZTJ2 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 30571708F8CEEF0F

FASTA74184,062
        10         20         30         40         50         60 
MNAPDKTGTR RRAIPAAVDL DALIRAEHRD PFSILGPHGD GGSGQYVRAY LPAALSVRLL 

        70         80         90        100        110        120 
ARDDGRELGE MEMSEVPGFF VGHLEHPQPY LLKINWAGGE QITEDPYSFG PLLGEMDLYL 

       130        140        150        160        170        180 
FAEGNHRDLS SCLGAQVTSV DGVDGVRFAV WAPNARRVSV VGSFNSWDGR RHPMRMRHPT 

       190        200        210        220        230        240 
GVWEIFVPRL QPGEVYKYEI LGAHGILPLK SDPMALSTTL PPDTASKIAA PLQFEWNDQE 

       250        260        270        280        290        300 
WLQSRAGRHE VTAPLSIYEL HAGSWQMEQV EDNQWRQYNW RELADRLIPY VKELGFTHIE 

       310        320        330        340        350        360 
LMPIMEHPFG GSWGYQLLAQ FAPTARYGSP EDFAFFVDAC HRAEIGVILD WVPAHFPTDT 

       370        380        390        400        410        420 
HGLAQFDGTC LYEYADPKEG FHQDWNTLIY NLGRTEVHGF MLASALHWLK HYHIDGLRVD 

       430        440        450        460        470        480 
AVASMLYRDY SRNAGEWVTN RFGGRENLEA IDFLRHLNDV VALEAPGTMV IAEESTAWPG 

       490        500        510        520        530        540 
VSEPTQQGGL GFNYKWNMGW MHDSLQYMEE DPINRGHHHG KLSFSLVYAW SERFVLPISH 

       550        560        570        580        590        600 
DEVVHGKHSL IDKMPGDRWQ KFANLRAYLS FMWTHPGKKL LFMGCEFGQW REWNHDRELD 

       610        620        630        640        650        660 
WYLMQYAEHV GVKNLVGDLN RLYREEKALH ERDADPAGFQ WLVGDDSANS VFAYLRWSND 

       670        680        690        700        710        720 
GEPLLVVANM TPVPRLDYRL GAPMRGAWTE LLNSDAETYA GSNFGNGGEV MTEAEPAHGM 

       730        740 
EDSLVLNLPP LAVLILKPKK D 

« Hide

References

[1]"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000."
Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E.
Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B728a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000075 Genomic DNA. Translation: AAY37530.1.
RefSeqYP_235568.1. NC_007005.1.

3D structure databases

ProteinModelPortalQ4ZTJ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205918.Psyr_2491.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY37530; AAY37530; Psyr_2491.
GeneID3368008.
KEGGpsb:Psyr_2491.
PATRIC19985570. VBIPseSyr42314_2551.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycPSYR205918:GJ94-2533-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PSEU2
AccessionPrimary (citable) accession number: Q4ZTJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries