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Q4ZTJ2

- GLGB_PSEU2

UniProt

Q4ZTJ2 - GLGB_PSEU2

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene
glgB, Psyr_2491
Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei420 – 4201Nucleophile By similarity
Active sitei473 – 4731Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciPSYR205918:GJ94-2533-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:Psyr_2491
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
ProteomesiUP000000426: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7417411,4-alpha-glucan branching enzyme GlgBUniRule annotationPRO_0000260680Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi205918.Psyr_2491.

Structurei

3D structure databases

ProteinModelPortaliQ4ZTJ2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4ZTJ2-1 [UniParc]FASTAAdd to Basket

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MNAPDKTGTR RRAIPAAVDL DALIRAEHRD PFSILGPHGD GGSGQYVRAY    50
LPAALSVRLL ARDDGRELGE MEMSEVPGFF VGHLEHPQPY LLKINWAGGE 100
QITEDPYSFG PLLGEMDLYL FAEGNHRDLS SCLGAQVTSV DGVDGVRFAV 150
WAPNARRVSV VGSFNSWDGR RHPMRMRHPT GVWEIFVPRL QPGEVYKYEI 200
LGAHGILPLK SDPMALSTTL PPDTASKIAA PLQFEWNDQE WLQSRAGRHE 250
VTAPLSIYEL HAGSWQMEQV EDNQWRQYNW RELADRLIPY VKELGFTHIE 300
LMPIMEHPFG GSWGYQLLAQ FAPTARYGSP EDFAFFVDAC HRAEIGVILD 350
WVPAHFPTDT HGLAQFDGTC LYEYADPKEG FHQDWNTLIY NLGRTEVHGF 400
MLASALHWLK HYHIDGLRVD AVASMLYRDY SRNAGEWVTN RFGGRENLEA 450
IDFLRHLNDV VALEAPGTMV IAEESTAWPG VSEPTQQGGL GFNYKWNMGW 500
MHDSLQYMEE DPINRGHHHG KLSFSLVYAW SERFVLPISH DEVVHGKHSL 550
IDKMPGDRWQ KFANLRAYLS FMWTHPGKKL LFMGCEFGQW REWNHDRELD 600
WYLMQYAEHV GVKNLVGDLN RLYREEKALH ERDADPAGFQ WLVGDDSANS 650
VFAYLRWSND GEPLLVVANM TPVPRLDYRL GAPMRGAWTE LLNSDAETYA 700
GSNFGNGGEV MTEAEPAHGM EDSLVLNLPP LAVLILKPKK D 741
Length:741
Mass (Da):84,062
Last modified:June 7, 2005 - v1
Checksum:i30571708F8CEEF0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY37530.1.
RefSeqiWP_011267721.1. NC_007005.1.
YP_235568.1. NC_007005.1.

Genome annotation databases

EnsemblBacteriaiAAY37530; AAY37530; Psyr_2491.
GeneIDi3368008.
KEGGipsb:Psyr_2491.
PATRICi19985570. VBIPseSyr42314_2551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY37530.1 .
RefSeqi WP_011267721.1. NC_007005.1.
YP_235568.1. NC_007005.1.

3D structure databases

ProteinModelPortali Q4ZTJ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205918.Psyr_2491.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY37530 ; AAY37530 ; Psyr_2491 .
GeneIDi 3368008.
KEGGi psb:Psyr_2491.
PATRICi 19985570. VBIPseSyr42314_2551.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci PSYR205918:GJ94-2533-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B728a.

Entry informationi

Entry nameiGLGB_PSEU2
AccessioniPrimary (citable) accession number: Q4ZTJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 7, 2005
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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