Reviewed, UniProtKB/Swiss-Prot Q4ZTG0 (DNAE2_PSEU2)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Psyr_2523

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	1031 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1031	1031	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103391

Sequences

	Sequence		Length	Mass (Da)
	Q4ZTG0-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 21A85A477C7FC6E8	FASTA	1,031	116,142
	10 20 30 40 50 60 MTREYAELHC LSNFSFQRGA SSARELFERA LRHGYKALAI TDECTLAGIV RAWQASKSTG 70 80 90 100 110 120 LPLIVGSEMH IENGPKVVLL VENQAGYEAL CKLITVARRR AGKGEYRVLR EDFEPAPDGL 130 140 150 160 170 180 LALWLPDLDG NAQACLAGGR WLRERFAERL WLGVGLHRGP DDEQRLADLL ALAQSLGVPA 190 200 210 220 230 240 VASGDVHMHA RGRRALQDTM TAIRHHTTVA EAGHLLFANG ERHLRPLDAL SEHYPDWLLA 250 260 270 280 290 300 ESVRIARRCT FDLGDLKYEY PHELVPKGQT STSWLRELTE RGVRRRWPGG LTPATRAQVE 310 320 330 340 350 360 KELALIAEKK FDSYFLTVHD IVEFARSQHI LCQGRGSAAN SAVCYALGIT ELNPEQSNLL 370 380 390 400 410 420 FERFISRERN EPPDIDVDFE HDRREEVIQY IFRRYGRGRA ALTAVASTYH GSGAMRDVAK 430 440 450 460 470 480 VLGLPPEQIN ALAEAFSRWS DSLPSPERLR EYGFDADTPI LKRVLALTGE LIGFPRHLSQ 490 500 510 520 530 540 HPGGFVISEH PLETLVPVEN AAMADRTIIQ WDKDDLDLVG LLKVDILALG MLSALRRTFD 550 560 570 580 590 600 LVHLHRGQRW TLATLPGDDR KTYEMISRAD TIGVFQIESR AQMAMLPRLR PEKFYDLVIE 610 620 630 640 650 660 VAIVRPGPIQ GDMVHPYLRR RNGEEDVTYP PKLESVFKRT LGVPLFQEQV MEVAILAADY 670 680 690 700 710 720 TPGEADELRR AMAAWKRHGG LEPHRERLRT GMLKNGYEAD FADRIFEQIK GFGSYGFPES 730 740 750 760 770 780 HAASFALLTY ASCWLKCHEP AAFTCALINS WPMGFYSPDQ LLQDARRHHI EIRPVDVRYS 790 800 810 820 830 840 DWDCSLEPLD HPDRTRNLAI RLGLRMVRSF REEDALRIEV ARAKRPFVDA TDLTLRAELD 850 860 870 880 890 900 ARAAEALADS GALRGLIGHR HRARWEVAGV EAQRPLFDDL PSEETQVTLP LPTVAEDLVA 910 920 930 940 950 960 DYTTLGTTLG PHPLALLRRQ LAAKRFRSSQ DLLHLENDRT LSVAGLVIGR QRPGTASGVT 970 980 990 1000 1010 1020 FVTLEDEFGM VNVVVWRDLA ERQRKVLVGS QLLQVFGRLE SKSGVRHLIA QRLYDLTPLL 1030 TGLDVRSRDF Q

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY37562.1.
RefSeq	YP_235600.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZTG0.
Genome annotation databases
GeneID	3368040.
GenomeReviews	Gene locus Psyr_2523 in contig CP000075_GR.
KEGG	psb:Psyr_2523.
NMPDR	fig|205918.4.peg.2996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_2523-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEU2

Accession

Primary (citable) accession number: Q4ZTG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)