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Q4ZRA0

- FADB_PSEU2

UniProt

Q4ZRA0 - FADB_PSEU2

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadB, Psyr_3290
Organism
Pseudomonas syringae pv. syringae (strain B728a)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate By similarity.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei120 – 1201Important for catalytic activity By similarity
Sitei140 – 1401Important for catalytic activity By similarity
Binding sitei297 – 2971Substrate By similarity
Binding sitei325 – 3251NAD; via amide nitrogen By similarity
Binding sitei344 – 3441NAD By similarity
Binding sitei408 – 4081NAD By similarity
Active sitei451 – 4511For 3-hydroxyacyl-CoA dehydrogenase activity By similarity
Binding sitei454 – 4541NAD By similarity
Binding sitei501 – 5011Substrate By similarity
Binding sitei660 – 6601Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi401 – 4033NAD By similarity
Nucleotide bindingi428 – 4303NAD By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-HAMAP
  5. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPSYR205918:GJ94-3342-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadB
Ordered Locus Names:Psyr_3290
OrganismiPseudomonas syringae pv. syringae (strain B728a)
Taxonomic identifieri205918 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae
ProteomesiUP000000426: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. fatty acid beta-oxidation multienzyme complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Fatty acid oxidation complex subunit alphaUniRule annotation
PRO_0000109281Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA) By similarity.

Protein-protein interaction databases

STRINGi205918.Psyr_3290.

Structurei

3D structure databases

ProteinModelPortaliQ4ZRA0.
SMRiQ4ZRA0. Positions 1-715.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydratase/isomerase By similarity
Add
BLAST
Regioni312 – 7214103-hydroxyacyl-CoA dehydrogenase By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261344.
KOiK01825.
OMAiNPIVVND.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4ZRA0-1 [UniParc]FASTAAdd to Basket

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MIYEGKAITV KALESGIVEL NFDLKGESVN KFNRLTLNEF RQAVDAVKAD    50
ASVKGVIVTS GKDSFIVGAD ITEFVDNFKL PEAELVAGNL EANRIFSDFE 100
DLNVPTVVAI NGIALGGGLE MCLAADYRVM ASSARIGLPE VKLGLYPGFG 150
GTVRLPRIIG ADNAIEWIAS GKENAAEDAL KVGAVDAVVA PEKLQAAALD 200
LIQRAISGEF DYKAKRQPKL DKLKLNAIEQ MMAFETAKGF VAGQAGPNYP 250
APVEAIKTIQ KAANFGRDKA LEIEAAGFVK MAKTPAAQSL IGLFLNDQEL 300
KKKARGYDKI AKDVKQAAVL GAGIMGGGIA YQSAVKGTPI LMKDIREEAI 350
QLGLNEASKL LGGRLEKGRL TAAKMAEALN AIRPTLSYGD FGNVDLVVEA 400
VVENPKVKQA VLAEVEANVG ENTILASNTS TISISLLAQA LKRPENFVGM 450
HFFNPVHMMP LVEVIRGEKS SEEAVATTVA YAKKMGKNPI VVNDCPGFLV 500
NRVLFPYFGG FARLVSAGVD FVRIDKVMEK FGWPMGPAYL MDVVGIDTGH 550
HGRDVMAEGF PDRMKDDRRS AIDALYDAKR LGQKNGKGFY AYETDKKGKP 600
KKVNDPAVLD VLKPIVYEQR EVSDEDIVNW MMIPLCLETV RCLEDGIVET 650
AAEADMGLIY GIGFPPFRGG ALRYIDSIGV AEFVALADQY AELGALYQPT 700
AKLREMAANG QSFFGQASSE E 721
Length:721
Mass (Da):77,709
Last modified:June 7, 2005 - v1
Checksum:i5C9890C2DCCF54EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY38322.1.
RefSeqiYP_236360.1. NC_007005.1.

Genome annotation databases

EnsemblBacteriaiAAY38322; AAY38322; Psyr_3290.
GeneIDi3368817.
KEGGipsb:Psyr_3290.
PATRICi19987216. VBIPseSyr42314_3364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000075 Genomic DNA. Translation: AAY38322.1 .
RefSeqi YP_236360.1. NC_007005.1.

3D structure databases

ProteinModelPortali Q4ZRA0.
SMRi Q4ZRA0. Positions 1-715.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205918.Psyr_3290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY38322 ; AAY38322 ; Psyr_3290 .
GeneIDi 3368817.
KEGGi psb:Psyr_3290.
PATRICi 19987216. VBIPseSyr42314_3364.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261344.
KOi K01825.
OMAi NPIVVND.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci PSYR205918:GJ94-3342-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01621. FadB.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02437. FadB. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B728a.

Entry informationi

Entry nameiFADB_PSEU2
AccessioniPrimary (citable) accession number: Q4ZRA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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