Q4ZR64 (HGD_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	Psyr_3326

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 1 › Pseudomonas syringae › Pseudomonas syringae pv. syringae

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000019536
Sites
	Metal binding	332	1	Iron By similarity
	Metal binding	338	1	Iron By similarity
	Metal binding	368	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZR64 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: DD5AC35417020D4E	FASTA	434	48,391
	10 20 30 40 50 60 MAIHSSSDAL VYQSGFGNQF SSEALPDALP VGQNSPQKHP LGLYAEQFSG TAFTVARSEA 70 80 90 100 110 120 RRTWLYRIKP SAAHSRYQRM DRQMAGQEPG PINPNRLRWN AFDIPAAPTD FIDGLIPLAS 130 140 150 160 170 180 TSAAEQAEGV SVYLYTANTS MQRAFFSADG EWLVVPQQGR LRIVTELGLL DIEPLEIAVL 190 200 210 220 230 240 PRGLKFCVQL LDSSARGYLC ENHGCALRLP ELGPIGSNGL ANSRDFLTPV AWFEDSRQPM 250 260 270 280 290 300 QLVQKFLGEL WSTQLEHSPF DVVGWHGNNV PYKYDLRRFN TIGTVSYDHP DPSIFTVLTS 310 320 330 340 350 360 PGAIHGQANI DFVIFPPRWM VAENTFRPPW FHRNLMNEFM GLIDGAYDAK AEGFMPGGAS 370 380 390 400 410 420 LHNCMSAHGP DNVTAEKAIA AELKPHRIDN TMAFMFETGK VLRPSRHALD CPQLQTDYDA 430 CWKDMARTFT QEPR

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B728a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY38358.1.
RefSeq	YP_236396.1. NC_007005.1.
3D structure databases
HSSP	HSSP built from PDB template 1EYB based on UniProtKB Q93099.
ModBase	Search...
Protein-protein interaction databases
STRING	205918.Psyr_3326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY38358; AAY38358; Psyr_3326.
GeneID	3368853.
KEGG	psb:Psyr_3326.
PATRIC	19987294. VBIPseSyr42314_3404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	PSYR205918:GJ94-3378-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEU2

Accession

Primary (citable) accession number: Q4ZR64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program