Reviewed, UniProtKB/Swiss-Prot Q4ZQH8 (ASTD_PSEU2)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	Psyr_3562

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262418
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZQH8-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 7080FD6B30E662A8	FASTA	488	51,332
	10 20 30 40 50 60 MNSLYIAGVW QDGQGEVVNS LNPVTQQVLW SGRGASAAQV EQAVQAARQA FPGWALLSLD 70 80 90 100 110 120 QRIAVLEAFA ARLKHHADAL AQCIGEETGK PLWESATEVT SMVNKIAISV QSYRERTGEK 130 140 150 160 170 180 SGPLGDATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNVVLFKP SELTPKVAEL 190 200 210 220 230 240 TVKCWIEAGL PAGVLNLLQG GRETGIALAA NPGIDGLFFT GSSRTGNALH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LIVDQVQDID AAVYTIIQSA FISAGQRCTC ARRLLVPEGD WGDALLARLV 310 320 330 340 350 360 GVSATIEAGA FDQQPAPFMG SVISLEAARA LLDAQRNLLA NGALTLLEMR QPQPGAALLT 370 380 390 400 410 420 PGIIDVSAVP ERPDEELFGP LLQVIRYAGF DAAIAEANAT RYGLAAGLLS DSEARYQQFW 430 440 450 460 470 480 LHSRAGIVNW NKPLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL EAGSLTLPAT LTPGIRLS

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000075 Genomic DNA. Translation: AAY38594.1.
RefSeq	YP_236632.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZQH8.
Genome annotation databases
GeneID	3369093.
GenomeReviews	Gene locus Psyr_3562 in contig CP000075_GR.
KEGG	psb:Psyr_3562.
NMPDR	fig|205918.4.peg.3999.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4ZQH8.
OMA	EMTQPQA.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_3562-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEU2

Accession

Primary (citable) accession number: Q4ZQH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)