Reviewed, UniProtKB/Swiss-Prot Q4ZNG3 (RLMG_PSEU2)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	Psyr_4279

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366486

Sequences

	Sequence		Length	Mass (Da)
	Q4ZNG3-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 29A65FFE4A4B1AEB	FASTA	374	40,736
	10 20 30 40 50 60 MPLLISPFAE LDLIRQPEQQ DEPLQAFDAA DEYLLNHVAQ AGLSLPSRVL VLNDSFGALA 70 80 90 100 110 120 ASLAPHATVI SSTDSFLAAQ ALEKNLARNG MSYDAVPHIP ASEALQGPFD WVLIRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGARV IAAAMVKHLP RSAGDLLEEY VGPVQASLAV KKARLLFATP 190 200 210 220 230 240 QPMEVRTSPY PTRYRLEEPA IELLNHANVF CRDGLDIGTR AFLPHLPKNL GTARVADLGC 250 260 270 280 290 300 GNGVLAIASA LDNPQAHYTL VDESFMAVKS AAENWRATLG DRDVRVRAGD GLEMQEPDSL 310 320 330 340 350 360 DVVLCNPPFH QQQVVGDFLA WRMFLQARAA LVTGGALYIV GNRHLGYHTK LSRLFRGVEQ 370 VAATPKFVIL KARK

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY39309.1.
RefSeq	YP_237347.1.
3D structure databases
SMR	Q4ZNG3. Positions 26-373.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZNG3.
Genome annotation databases
GeneID	3369819.
GenomeReviews	Gene locus Psyr_4279 in contig CP000075_GR.
KEGG	psb:Psyr_4279.
NMPDR	fig|205918.4.peg.4714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	CGNGVLG.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_4279-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEU2

Accession

Primary (citable) accession number: Q4ZNG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)