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Q4ZN90 (SYL_PSEU2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Psyr_4352
OrganismPseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]
Taxonomic identifier205918 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas syringae

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009401

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ZN90 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 58F1A797A5E213A8

FASTA86896,923
        10         20         30         40         50         60 
MHELYQPREI EAAAQTFWDE QKSFEVSEQP GKDTFYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPLGWD AFGMPAENAA IDNNVAPAKW TYENIDYMKT QLKSLGLAVD 

       130        140        150        160        170        180 
WSREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITAYAD ELLESLDELP GWPEQVKTMQ RNWIGRSRGM EVQFPYDQAS 

       250        260        270        280        290        300 
IGEAGALKVF TTRPDTLMGA TYVAVAAEHP LATLAAQGNP ALQAFIDECK GGSVAEADVA 

       310        320        330        340        350        360 
TQEKKGQATS LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF EFATQYGLPI 

       370        380        390        400        410        420 
KPVVRTSAGD QTPAPWQPAY GEHGELINSG EFTGLTFQDA FDAIEAALVK KSLGQSRTQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPIV HCDTCGDVPV PEDQLPVVLP EDVVPDGAGS PLARMPEFYE 

       490        500        510        520        530        540 
CSCPKCGAPA KRETDTMDTF VESSWYYARY ASPHYEGGLV EPNAANHWLP VDQYIGGIEH 

       550        560        570        580        590        600 
AILHLLYARF FHKLMRDEGL VTSNEPFKNL LTQGMVNAET YFRMETSGKK TWINPADVTL 

       610        620        630        640        650        660 
ERDAKAKVIS ARLTSDGLPV EIGGTEKMSK SKKNGIDPQT MIDQYGADTC RLFMMFASPP 

       670        680        690        700        710        720 
DMSLEWSDSG VEGSHRFLRR VWRLAQAHVT QGPSTGLDVA ALSDEQKAIR RAIHQAIRQA 

       730        740        750        760        770        780 
SQDIGQNQKF NTAVAQVMTL MNVLEKAPQD TPQDRALMQE GVETVALLLA PITPHISHEL 

       790        800        810        820        830        840 
WKQLGHNEPV IDAGWPAFDA SALVQDSLQL VIQVNGKLRG HIEMPASASR EEVEAAARVN 

       850        860 
ENVLRFTDGL TIRKVIVVPG KLVNIVAS 

« Hide

References

[1]"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000."
Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E.
Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B728a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000075 Genomic DNA. Translation: AAY39382.1.
RefSeqYP_237420.1. NC_007005.1.

3D structure databases

ProteinModelPortalQ4ZN90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205918.Psyr_4352.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY39382; AAY39382; Psyr_4352.
GeneID3369892.
KEGGpsb:Psyr_4352.
PATRIC19989493. VBIPseSyr42314_4492.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPSYR205918:GJ94-4417-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEU2
AccessionPrimary (citable) accession number: Q4ZN90
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 7, 2005
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries