Reviewed, UniProtKB/Swiss-Prot Q4ZM83 (GLYA2_PSEU2)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	Psyr_4712

Organism

Pseudomonas syringae pv. syringae (strain B728a) [Complete proteome] [HAMAP]

Taxonomic identifier

205918 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000235010
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ZM83-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 004FD60AEBBE4D16	FASTA	417	44,874
	10 20 30 40 50 60 MFSKQDQIQG YDDALLSAMN AEEQRQEDHI ELIASENYTS KRVMQAQGSG LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEH VDKVEQLAIE RARQLFGADY ANVQPHSGSQ ANAAVYLALL QAGDTVLGMS 130 140 150 160 170 180 LAHGGHLTHG AKVSFSGKLY NAVQYGIDTA TGLIDYDEVE RIAVECQPKM IIAGFSAYSK 190 200 210 220 230 240 TLDFPRFRAI ADKVGAYLFV DMAHVAGLVA AGLYPNPLPY ADVVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LARANEELEK KFNSAVFPGG QGGPLMHVIA AKAVCFKEAM EPGFKAYQQQ VIDNAQAMAQ 310 320 330 340 350 360 VFIDRGFDVV SGGTDNHLFL VSLIRQGLTG KDADAALGRA HITVNKNSVP NDPQSPFVTS 370 380 390 400 410 GLRIGTPAVT TRGFKVTQCV ELAGWICDIL DNLGDADVEA DVASQVAALC ADFPVYR

References

[1]

"Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000." Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A., Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S., Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M., Kyrpides N.C., Ivanova N., Lindow S.E. Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005) [PubMed: 16043691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000075 Genomic DNA. Translation: AAY39739.1.
RefSeq	YP_237777.1.
3D structure databases
SMR	Q4ZM83. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4ZM83.
Genome annotation databases
GeneID	3370262.
GenomeReviews	Gene locus Psyr_4712 in contig CP000075_GR.
KEGG	psb:Psyr_4712.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0112.
HOGENOM	HBG301263.
OMA	QTIARLI.
Enzyme and pathway databases
BioCyc	PSYR205918:PSYR_4712-MONOMER.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_PSEU2

Accession

Primary (citable) accession number: Q4ZM83

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)