Reviewed, UniProtKB/Swiss-Prot Q4ZG55 (GREB1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein GREB1
Alternative name(s):
    Gene regulated in breast cancer 1 protein

Gene names

Name:	GREB1
Synonyms:	KIAA0575

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1949 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Tissue specificity	Expressed in proliferating prostatic tissue and prostate cancer.
Induction	Estrogen-responsive; regulated by estrogen receptors. Regulated by androgens. The regulatory region of the gene contains 3 estrogen-responsive elements.
Sequence similarities	Belongs to the GREB1 family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4ZG55-1)

Also known as: GREB1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4ZG55-2)

Also known as: GREB1b;

The sequence of this isoform differs from the canonical sequence as follows:
     450-457: ADQVPLME → HQIRNPDV
     458-1949: Missing.

Isoform 3 (identifier: Q4ZG55-3)

Also known as: GREB1c;

The sequence of this isoform differs from the canonical sequence as follows:
     387-409: GHGNFPYLCGNLNDVVVSPLLYT → VFVNGATQMVALGPAEPASPRSL
     410-1949: Missing.

Isoform 4 (identifier: Q4ZG55-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1002: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1949	1949	Protein GREB1		PRO_0000320944
Regions
	Transmembrane	1868 – 1888	21	Potential
	Compositional bias	1109 – 1232	124	Ser-rich
Natural variations
	Alternative sequence	1 – 1002	1002	Missing in isoform 4.		VSP_031757
	Alternative sequence	387 – 409	23	GHGNF…PLLYT → VFVNGATQMVALGPAEPASP RSL in isoform 3.		VSP_031758
	Alternative sequence	410 – 1949	1540	Missing in isoform 3.		VSP_031759
	Alternative sequence	450 – 457	8	ADQVPLME → HQIRNPDV in isoform 2.		VSP_031760
	Alternative sequence	458 – 1949	1492	Missing in isoform 2.		VSP_031761
	Natural variant	77	1	N → T: dbSNP rs10929757.		VAR_039313
	Natural variant	122	1	V → A: dbSNP rs4669751.		VAR_039314
	Natural variant	346	1	V → M: dbSNP rs6744817.		VAR_039315
	Natural variant	973	1	R → Q: dbSNP rs3762579.		VAR_039316
	Natural variant	1463	1	Y → C: dbSNP rs11695925.		VAR_039317
	Natural variant	1687	1	D → N: dbSNP rs2304402.		VAR_039318
	Natural variant	1814	1	L → V: dbSNP rs34955282.		VAR_039319
Experimental info
	Sequence conflict	829	1	I → S in AAG39036. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (GREB1a) [UniParc]. Last modified June 7, 2005. Version 1. Checksum: BA12C0535B166BC4	FASTA	1,949	216,467
	10 20 30 40 50 60 MGNSYAGQLK TTRFEEVLHN SIEASLRSNN LVPRPIFSQL YLEAEQQLAA LEGGSRVDNE 70 80 90 100 110 120 EEEEEGEGGL ETNGPPNPFQ LHPLPEGCCT TDGFCQAGKD LRLVSISNEP MDVPAGFLLV 130 140 150 160 170 180 GVKSPSLPDH LLVCAVDKRF LPDDNGHNAL LGFSGNCVGC GKKGFCYFTE FSNHINLKLT 190 200 210 220 230 240 TQPKKQKHLK YYLVRNAQGT LTKGPLICWK GSEFRSRQIP ASTCSSSLFP ALESTAAFPS 250 260 270 280 290 300 EPVPGTNPSI LMGAQQAGPA SDHPSLNAAM GPAVFNGKDS PKCQQLAKNN LLALPRPSAL 310 320 330 340 350 360 GILSNSGPPK KRHKGWSPES PSAPDGGCPQ GGGNRAKYES AGMSCVPQVG LVGPASVTFP 370 380 390 400 410 420 VVASGEPVSV PDNLLKICKA KPVIFKGHGN FPYLCGNLND VVVSPLLYTC YQNSQSVSRA 430 440 450 460 470 480 YEQYGASAIQ PISEEMQLLL TVYYLVQLAA DQVPLMEDLE QIFLRSWRES HLTEIRQYQQ 490 500 510 520 530 540 APPQPFPPAP SAAAPVTSAQ LPWLASLAAS SCNDSVHVIE CAYSLAEGLS EMFRLLVEGK 550 560 570 580 590 600 LAKTNYVVII CACRSAAIDS CIAVTGKYQA RILSESLLTP AEYQKEVNYE LVTGKVDSLG 610 620 630 640 650 660 AFFSTLCPEG DIDILLDKFH QENQGHISSS LAASSVTKAA SLDVSGTPVC TSYNLEPHSI 670 680 690 700 710 720 RPFQLAVAQK LLSHVCSIAD SSTQNLDLGS FEKVDFLICI PPSEVTYQQT LLHVWHSGVL 730 740 750 760 770 780 LELGLKKEHM TKQRVEQYVL KLDTEAQTKF KAFLQNSFQN PHTLFVLIHD HAHWDLVSST 790 800 810 820 830 840 VHNLYSQSDP SVGLVDRLLN CREVKEAPNI VTLHVTSFPY ALQTQHTLIS PYNEIHWPAS 850 860 870 880 890 900 CSNGVDLYHE NKKYFGLSEF IESTLSGHSL PLLRYDSSFE AMVTALGKRF PRLHSAVIRT 910 920 930 940 950 960 FVLVQHYAAA LMAVSGLPQM KNYTSVETLE ITQNLLNSPK QCPCGHGLMV LLRVPCSPLA 970 980 990 1000 1010 1020 VVAYERLAHV RARLALEEHF EIILGSPSSG VTVGKHFVKQ LRMWQKIEDV EWRPQTYLEL 1030 1040 1050 1060 1070 1080 EGLPCILIFS GMDPHGESLP RSLRYCDLRL INSSCLVRTA LEQELGLAAY FVSNEVPLEK 1090 1100 1110 1120 1130 1140 GARNEALESD AEKLSSTDNE DEELGTEGST SEKRSPMKRE RSRSHDSASS SLSSKASGSA 1150 1160 1170 1180 1190 1200 LGGESSAQPT ALPQGEHARS PQPRGPAEEG RAPGEKQRPR ASQGPPSAIS RHSPGPTPQP 1210 1220 1230 1240 1250 1260 DCSLRTGQRS VQVSVTSSCS QLSSSSGSSS SSVAPAAGTW VLQASQCSLT KACRQPPIVF 1270 1280 1290 1300 1310 1320 LPKLVYDMVV STDSSGLPKA ASLLPSPSVM WASSFRPLLS KTMTSTEQSL YYRQWTVPRP 1330 1340 1350 1360 1370 1380 SHMDYGNRAE GRVDGFHPRR LLLSGPPQIG KTGAYLQFLS VLSRMLVRLT EVDVYDEEEI 1390 1400 1410 1420 1430 1440 NINLREESDW HYLQLSDPWP DLELFKKLPF DYIIHDPKYE DASLICSHYQ GIKSEDRGMS 1450 1460 1470 1480 1490 1500 RKPEDLYVRR QTARMRLSKY AAYNTYHHCE QCHQYMGFHP RYQLYESTLH AFAFSYSMLG 1510 1520 1530 1540 1550 1560 EEIQLHFIIP KSKEHHFVFS QPGGQLESMR LPLVTDKSHE YIKSPTFTPT TGRHEHGLFN 1570 1580 1590 1600 1610 1620 LYHAMDGASH LHVLVVKEYE MAIYKKYWPN HIMLVLPSIF NSAGVGAAHF LIKELSYHNL 1630 1640 1650 1660 1670 1680 ELERNRQEEL GIKPQDIWPF IVISDDSCVM WNVVDVNSAG ERSREFSWSE RNVSLKHIMQ 1690 1700 1710 1720 1730 1740 HIEAAPDIMH YALLGLRKWS SKTRASEVQE PFSRCHVHNF IILNVDLTQN VQYNQNRFLC 1750 1760 1770 1780 1790 1800 DDVDFNLRVH SAGLLLCRFN RFSVMKKQIV VGGHRSFHIT SKVSDNSAAV VPAQYICAPD 1810 1820 1830 1840 1850 1860 SKHTFLAAPA QLLLEKFLQH HSHLFFPLSL KNHDHPVLSV DCYLNLGSQI SVCYVSSRPH 1870 1880 1890 1900 1910 1920 SLNISCSDLL FSGLLLYLCD SFVGASFLKK FHFLKGATLC VICQDRSSLR QTVVRLELED 1930 1940 EWQFRLRDEF QTANAREDRP LFFLTGRHI
	Isoform 2 (GREB1b) [UniParc]. Checksum: 2C0028B0ECC23CB4		457	48,935
	10 20 30 40 50 60 MGNSYAGQLK TTRFEEVLHN SIEASLRSNN LVPRPIFSQL YLEAEQQLAA LEGGSRVDNE 70 80 90 100 110 120 EEEEEGEGGL ETNGPPNPFQ LHPLPEGCCT TDGFCQAGKD LRLVSISNEP MDVPAGFLLV 130 140 150 160 170 180 GVKSPSLPDH LLVCAVDKRF LPDDNGHNAL LGFSGNCVGC GKKGFCYFTE FSNHINLKLT 190 200 210 220 230 240 TQPKKQKHLK YYLVRNAQGT LTKGPLICWK GSEFRSRQIP ASTCSSSLFP ALESTAAFPS 250 260 270 280 290 300 EPVPGTNPSI LMGAQQAGPA SDHPSLNAAM GPAVFNGKDS PKCQQLAKNN LLALPRPSAL 310 320 330 340 350 360 GILSNSGPPK KRHKGWSPES PSAPDGGCPQ GGGNRAKYES AGMSCVPQVG LVGPASVTFP 370 380 390 400 410 420 VVASGEPVSV PDNLLKICKA KPVIFKGHGN FPYLCGNLND VVVSPLLYTC YQNSQSVSRA 430 440 450 YEQYGASAIQ PISEEMQLLL TVYYLVQLAH QIRNPDV
	Isoform 3 (GREB1c) [UniParc]. Checksum: 0E03659DC466B4C0		409	43,253
	10 20 30 40 50 60 MGNSYAGQLK TTRFEEVLHN SIEASLRSNN LVPRPIFSQL YLEAEQQLAA LEGGSRVDNE 70 80 90 100 110 120 EEEEEGEGGL ETNGPPNPFQ LHPLPEGCCT TDGFCQAGKD LRLVSISNEP MDVPAGFLLV 130 140 150 160 170 180 GVKSPSLPDH LLVCAVDKRF LPDDNGHNAL LGFSGNCVGC GKKGFCYFTE FSNHINLKLT 190 200 210 220 230 240 TQPKKQKHLK YYLVRNAQGT LTKGPLICWK GSEFRSRQIP ASTCSSSLFP ALESTAAFPS 250 260 270 280 290 300 EPVPGTNPSI LMGAQQAGPA SDHPSLNAAM GPAVFNGKDS PKCQQLAKNN LLALPRPSAL 310 320 330 340 350 360 GILSNSGPPK KRHKGWSPES PSAPDGGCPQ GGGNRAKYES AGMSCVPQVG LVGPASVTFP 370 380 390 400 VVASGEPVSV PDNLLKICKA KPVIFKVFVN GATQMVALGP AEPASPRSL
	Isoform 4 [UniParc]. Checksum: 21023519D9B054E9		947	107,227
	10 20 30 40 50 60 MWQKIEDVEW RPQTYLELEG LPCILIFSGM DPHGESLPRS LRYCDLRLIN SSCLVRTALE 70 80 90 100 110 120 QELGLAAYFV SNEVPLEKGA RNEALESDAE KLSSTDNEDE ELGTEGSTSE KRSPMKRERS 130 140 150 160 170 180 RSHDSASSSL SSKASGSALG GESSAQPTAL PQGEHARSPQ PRGPAEEGRA PGEKQRPRAS 190 200 210 220 230 240 QGPPSAISRH SPGPTPQPDC SLRTGQRSVQ VSVTSSCSQL SSSSGSSSSS VAPAAGTWVL 250 260 270 280 290 300 QASQCSLTKA CRQPPIVFLP KLVYDMVVST DSSGLPKAAS LLPSPSVMWA SSFRPLLSKT 310 320 330 340 350 360 MTSTEQSLYY RQWTVPRPSH MDYGNRAEGR VDGFHPRRLL LSGPPQIGKT GAYLQFLSVL 370 380 390 400 410 420 SRMLVRLTEV DVYDEEEINI NLREESDWHY LQLSDPWPDL ELFKKLPFDY IIHDPKYEDA 430 440 450 460 470 480 SLICSHYQGI KSEDRGMSRK PEDLYVRRQT ARMRLSKYAA YNTYHHCEQC HQYMGFHPRY 490 500 510 520 530 540 QLYESTLHAF AFSYSMLGEE IQLHFIIPKS KEHHFVFSQP GGQLESMRLP LVTDKSHEYI 550 560 570 580 590 600 KSPTFTPTTG RHEHGLFNLY HAMDGASHLH VLVVKEYEMA IYKKYWPNHI MLVLPSIFNS 610 620 630 640 650 660 AGVGAAHFLI KELSYHNLEL ERNRQEELGI KPQDIWPFIV ISDDSCVMWN VVDVNSAGER 670 680 690 700 710 720 SREFSWSERN VSLKHIMQHI EAAPDIMHYA LLGLRKWSSK TRASEVQEPF SRCHVHNFII 730 740 750 760 770 780 LNVDLTQNVQ YNQNRFLCDD VDFNLRVHSA GLLLCRFNRF SVMKKQIVVG GHRSFHITSK 790 800 810 820 830 840 VSDNSAAVVP AQYICAPDSK HTFLAAPAQL LLEKFLQHHS HLFFPLSLKN HDHPVLSVDC 850 860 870 880 890 900 YLNLGSQISV CYVSSRPHSL NISCSDLLFS GLLLYLCDSF VGASFLKKFH FLKGATLCVI 910 920 930 940 CQDRSSLRQT VVRLELEDEW QFRLRDEFQT ANAREDRPLF FLTGRHI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PDZK1 and GREB1 are estrogen-regulated genes expressed in hormone-responsive breast cancer." Ghosh M.G., Thompson D.A., Weigel R.J. Cancer Res. 60:6367-6375(2000) [PubMed: 11103799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [MRNA] OF 1-1001 (ISOFORM 1). Tissue: Mammary carcinoma.
[2]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Brain.
[3]	Ohara O., Nagase T., Ishikawa K. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS THR-77 AND ASN-1687.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANTS THR-77 AND ASN-1687. Tissue: Skin.
[7]	"Discovery of estrogen receptor alpha target genes and response elements in breast tumor cells." Lin C.-Y., Stroem A., Vega V.B., Kong S.L., Yeo A.L., Thomsen J.S., Chan W.C., Doray B., Bangarusamy D.K., Ramasamy A., Vergara L.A., Tang S., Chong A., Bajic V.B., Miller L.D., Gustafsson J.-A., Liu E.T. Genome Biol. 5:RESEARCH0066.1-RESEARCH0066.18(2004) [PubMed: 15345050] [Abstract] Cited for: INDUCTION BY ESTROGEN.
[8]	"GREB 1 is a critical regulator of hormone dependent breast cancer growth." Rae J.M., Johnson M.D., Scheys J.O., Cordero K.E., Larios J.M., Lippman M.E. Breast Cancer Res. Treat. 92:141-149(2005) [PubMed: 15986123] [Abstract] Cited for: POSSIBLE FUNCTION, INDUCTION BY ESTROGEN.
[9]	"GREB1 is a novel androgen-regulated gene required for prostate cancer growth." Rae J.M., Johnson M.D., Cordero K.E., Scheys J.O., Larios J.M., Gottardis M.M., Pienta K.J., Lippman M.E. Prostate 66:886-894(2006) [PubMed: 16496412] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[10]	"Regulation of GREB1 transcription by estrogen receptor alpha through a multipartite enhancer spread over 20 kb of upstream flanking sequences." Deschenes J., Bourdeau V., White J.H., Mader S. J. Biol. Chem. 282:17335-17339(2007) [PubMed: 17463000] [Abstract] Cited for: INDUCTION BY ESTROGEN.
[11]	"Long-range activation of GREB1 by estrogen receptor via three distal consensus estrogen-responsive elements in breast cancer cells." Sun J., Nawaz Z., Slingerland J.M. Mol. Endocrinol. 21:2651-2662(2007) [PubMed: 17666587] [Abstract] Cited for: INDUCTION BY ESTROGEN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF245388 mRNA. Translation: AAG39036.1. AF245389 mRNA. Translation: AAG39037.1. AF245390 mRNA. Translation: AAG39038.1. AB011147 mRNA. Translation: BAA25501.2. Different initiation. AC011994 Genomic DNA. Translation: AAX88907.1. CH471053 Genomic DNA. Translation: EAX00927.1. CH471053 Genomic DNA. Translation: EAX00928.1. BC054502 mRNA. Translation: AAH54502.1.
PIR	T00340.
RefSeq	NP_055483.2. NP_683701.2.
UniGene	Hs.467733
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000196208. Homo sapiens. [Contig view]
GeneID	9687.
KEGG	hsa:9687.
Organism-specific databases
MIM	611736. gene.
HUGE	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q4ZG55.
HOVERGEN	Q4ZG55.
Gene expression databases
ArrayExpress	Q4ZG55.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	36380.
SOURCE	Search...

Entry information

Entry name

GREB1_HUMAN

Accession

Primary (citable) accession number: Q4ZG55
Secondary accession number(s): A6NHD0 , A6NKN0, O60321, Q7Z5S2, Q9H2Q6, Q9H2Q7, Q9H2Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 7, 2005
Last modified:	November 4, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)