Reviewed, UniProtKB/Swiss-Prot Q4Z6R1 (CDC2H_PLABA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	CRK2
ORF Names:	PB000293.00.0

Organism

Plasmodium berghei (strain Anka)

Taxonomic identifier

5823 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Vinckeia)

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expressed at a low level during the asexual cell-cycle within the host erythrocytes. Also expressed in gametocytes and in stages during development in the mosquito. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Cell division control protein 2 homolog		PRO_0000232668
Regions
	Domain	4 – 284	281	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	125	1	Proton acceptor By similarity
	Binding site	32	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	158	1	Phosphothreonine; by CAK By similarity
Experimental info
	Sequence conflict	81	1	H → Q in CAA11849. Ref.1
	Sequence conflict	277 – 278	2	QA → YT in CAA11849. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q4Z6R1-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: B2765B6C3EE4139E	FASTA	288	32,963
	10 20 30 40 50 60 MEKYHGLEKI GEGTYGVVYK AQNSDGESFA LKKIRLEKED EGIPSTAIRE ISILKELRHS 70 80 90 100 110 120 NIVKLYDVIH AKKRLILVFE HLDQDLKKLI DVCDGGLESV TAKSFLLQLL NGIAYCHEHR 130 140 150 160 170 180 VLHRDLKPQN LLINREGELK IADFGLARAF GIPARRYTHE VVTLWYRAPD ILMGSKKYST 190 200 210 220 230 240 PIDIWSVGCI FAEMVNGRPL FPGASETDQL MRIFKILGTP NSQNWPDVFK LPKYDPNFPV 250 260 270 280 YNPLPWETFI KGLDDTGIDL LSKMLKLDPN QRITAKQAIE HPYFKETN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei." Vinkenoog R., Speranca M.A., Ramesar J., Thomas A.W., del Portillo H.A., Janse C.J., Waters A.P. Mol. Biochem. Parasitol. 95:229-240(1998) [PubMed: 9803415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[2]	"A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses." Hall N., Karras M., Raine J.D., Carlton J.M., Kooij T.W.A., Berriman M., Florens L., Janssen C.S., Pain A., Christophides G.K., James K., Rutherford K., Harris B., Harris D., Churcher C.M., Quail M.A., Ormond D., Doggett J. , Trueman H.E., Mendoza J., Bidwell S.L., Rajandream M.A., Carucci D.J., Yates J.R. III, Kafatos F.C., Janse C.J., Barrell B.G., Turner C.M.R., Waters A.P., Sinden R.S. Science 307:82-86(2005) [PubMed: 15637271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224152 Genomic DNA. Translation: CAA11849.1. CAAI01000222 Genomic DNA. Translation: CAH93935.1.
RefSeq	XP_677202.1.
3D structure databases
SMR	Q4Z6R1. Positions 1-286.
ModBase	Search...
Genome annotation databases
GeneID	3425729.
KEGG	pbe:PB000293.00.0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2H_PLABA

Accession

Primary (citable) accession number: Q4Z6R1
Secondary accession number(s): O96820

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	July 5, 2005
Last modified:	December 15, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)