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Q4Z6R1 (CDC2H_PLABA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 2 homolog

EC=2.7.11.22
EC=2.7.11.23
Gene names
Name:CRK2
ORF Names:PB000293.00.0
OrganismPlasmodium berghei (strain Anka) [Reference proteome]
Taxonomic identifier5823 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Vinckeia)

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.

Subunit structure

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin By similarity.

Subcellular location

Cytoplasm By similarity.

Developmental stage

Expressed at a low level during the asexual cell-cycle within the host erythrocytes. Also expressed in gametocytes and in stages during development in the mosquito. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Cell division control protein 2 homolog
PRO_0000232668

Regions

Domain4 – 284281Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1251Proton acceptor By similarity
Binding site321ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine By similarity
Modified residue1581Phosphothreonine; by CAK By similarity

Experimental info

Sequence conflict811H → Q in CAA11849. Ref.1
Sequence conflict277 – 2782QA → YT in CAA11849. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q4Z6R1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: B2765B6C3EE4139E

FASTA28832,963
        10         20         30         40         50         60 
MEKYHGLEKI GEGTYGVVYK AQNSDGESFA LKKIRLEKED EGIPSTAIRE ISILKELRHS 

        70         80         90        100        110        120 
NIVKLYDVIH AKKRLILVFE HLDQDLKKLI DVCDGGLESV TAKSFLLQLL NGIAYCHEHR 

       130        140        150        160        170        180 
VLHRDLKPQN LLINREGELK IADFGLARAF GIPARRYTHE VVTLWYRAPD ILMGSKKYST 

       190        200        210        220        230        240 
PIDIWSVGCI FAEMVNGRPL FPGASETDQL MRIFKILGTP NSQNWPDVFK LPKYDPNFPV 

       250        260        270        280 
YNPLPWETFI KGLDDTGIDL LSKMLKLDPN QRITAKQAIE HPYFKETN 

« Hide

References

« Hide 'large scale' references
[1]"Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei."
Vinkenoog R., Speranca M.A., Ramesar J., Thomas A.W., del Portillo H.A., Janse C.J., Waters A.P.
Mol. Biochem. Parasitol. 95:229-240(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[2]"A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses."
Hall N., Karras M., Raine J.D., Carlton J.M., Kooij T.W.A., Berriman M., Florens L., Janssen C.S., Pain A., Christophides G.K., James K., Rutherford K., Harris B., Harris D., Churcher C.M., Quail M.A., Ormond D., Doggett J. expand/collapse author list , Trueman H.E., Mendoza J., Bidwell S.L., Rajandream M.A., Carucci D.J., Yates J.R. III, Kafatos F.C., Janse C.J., Barrell B.G., Turner C.M.R., Waters A.P., Sinden R.S.
Science 307:82-86(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ANKA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ224152 Genomic DNA. Translation: CAA11849.1.
CAAI01000222 Genomic DNA. Translation: CAH93935.1.
RefSeqXP_677202.1. XM_672110.1.

3D structure databases

ProteinModelPortalQ4Z6R1.
SMRQ4Z6R1. Positions 1-286.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ4Z6R1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsPBANKA_113320:mRNA; PBANKA_113320:pep; PBANKA_113320.
GeneID3425729.
KEGGpbe:PB000293.00.0.

Organism-specific databases

EuPathDBPlasmoDB:PBANKA_113320.

Phylogenomic databases

HOGENOMHOG000233024.
KOK04563.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDC2H_PLABA
AccessionPrimary (citable) accession number: Q4Z6R1
Secondary accession number(s): O96820
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families