Q4X180 (ATG15_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase atg15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	AFUA_2G10900

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Putative lipase atg15		PRO_0000090364
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 650	618	Lumenal Potential
	Compositional bias	471 – 632	162	Thr-rich
Sites
	Active site	320	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4X180 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 6B0C115DBF6141AA	FASTA	650	70,250
	10 20 30 40 50 60 MKSSRKRTKR RVLQDMSISG LLLSVALLPS VVSAQDHVYL DPSSSGSPYL GPQIPLTGLP 70 80 90 100 110 120 ALTGTHEFTL RHIYQRGTHD QPDLHRRLDI KPHTRLWAVS DDGLEKELVT FDTPLVASSS 130 140 150 160 170 180 PLTIQRLADR RLSVIEGYLA AARSRGEAVA LSPSEWVMDT LAGPNVTDKE SVLTFAKMTA 190 200 210 220 230 240 NDYIEEPGSG DWHYIHGRFN YSSSFGWQSD GLRGHIYADK TNSTIVISLK GTSPALFDGA 250 260 270 280 290 300 GTTTNDKIND NLFFSCCCGQ GGSYLWRQVC DCQQSAFTAN LTCIVEAMND ENRYYRAAID 310 320 330 340 350 360 LYSNVTDMYP DANVWLTGHS LGGAMSSLLG LTFGLPVVTF EAVPEALPAA RLGLPSPPGH 370 380 390 400 410 420 DPRLPQSRQY TGAYHFGHTA DPVYMGTCNG VGSICTWGGY AMESACHTGQ MCVYDTVEDK 430 440 450 460 470 480 GWRVALSTHR IEAVISDVLE VYEDIPPCAP EEECYDCELW KFFKSNGSES TTTSTTTTTT 490 500 510 520 530 540 APTTTRTSTC KTPGWWGCLD ESTTTTTTTS TTTTTTTTTT STCKTPGWFG CKDPTTTTEA 550 560 570 580 590 600 TAAPSVTTSI PTPTTYPTSS TSTCKDPGWF GCRDPPSTTA SITSSPSTTS TCDDPGFFWG 610 620 630 640 650 CYDESTTATH PITSGPSAPY STPSPTHEHT CTSSIFFGLI CVGSTGTELR

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000001 Genomic DNA. Translation: EAL93385.1.
RefSeq	XP_755423.1. XM_750330.1.
3D structure databases
ProteinModelPortal	Q4X180.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4X180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00004206; CADAFUAP00004206; CADAFUAG00004206.
GeneID	3513512.
GenomeReviews	Gene locus atg15 in contig CM000170_GR.
KEGG	afm:AFUA_2G10900.
Phylogenomic databases
eggNOG	fuNOG04745.
GeneTree	EFGT00050000002657.
HOGENOM	HBG398588.
OMA	EFTLRHI.
OrthoDB	EOG4F7RV2.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
KO	K01046.
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_ASPFU

Accession

Primary (citable) accession number: Q4X180

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2005
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program