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Protein

ATPase get3

Gene

get3

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631UniRule annotation
Binding sitei245 – 2451ATPUniRule annotation
Binding sitei272 – 2721ATP
Metal bindingi283 – 2831Zinc; shared with dimeric partnerUniRule annotation
Metal bindingi286 – 2861Zinc; shared with dimeric partnerUniRule annotation
Binding sitei320 – 3201ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 418ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase get3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:get3
ORF Names:AFUA_3G11350
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiFungiDB:Afu3g11350.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Endoplasmic reticulum UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381G → R: Abolishes ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340ATPase get3PRO_0000388189Add
BLAST

Proteomic databases

PRIDEiQ4WY07.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-48950N.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 214Combined sources
Beta strandi28 – 325Combined sources
Beta strandi34 – 385Combined sources
Helixi39 – 5113Combined sources
Beta strandi57 – 615Combined sources
Helixi67 – 726Combined sources
Beta strandi87 – 937Combined sources
Helixi100 – 1045Combined sources
Helixi134 – 15017Combined sources
Beta strandi153 – 1586Combined sources
Turni163 – 1664Combined sources
Helixi167 – 1704Combined sources
Helixi171 – 18515Combined sources
Helixi198 – 2014Combined sources
Helixi209 – 23022Combined sources
Turni233 – 2353Combined sources
Beta strandi236 – 2427Combined sources
Helixi246 – 26116Combined sources
Beta strandi266 – 2749Combined sources
Helixi284 – 30320Combined sources
Turni304 – 3063Combined sources
Beta strandi307 – 3137Combined sources
Helixi322 – 3309Combined sources
Turni331 – 3333Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IBGX-ray3.20A/B/C/D/E/F3-340[»]
ProteinModelPortaliQ4WY07.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4WY07.

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000197637.
InParanoidiQ4WY07.
KOiK01551.
OMAiYEIDTHN.
OrthoDBiEOG7XDBRV.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_03112. Asna1_Get3.
InterProiIPR025723. Anion-transp_ATPase-like_dom.
IPR016300. ATPase_ArsA/GET3.
IPR027542. ATPase_ArsA/GET3_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00345. GET3_arsA_TRC40. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4WY07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTAVVHGD DLMEPTLQSI LSQKTLRWIF VGGKGGVGKT TTSCSLAIQL
60 70 80 90 100
AKVRKSVLLI STDPAHNLSD AFGQKFGKEA RLVDGYSNLS AMEIDPNGSI
110 120 130 140 150
QDLLASGDSQ GDDPLAGLGM GNMMQDLAFS IPGVDEAMSF AEVLKQVKSL
160 170 180 190 200
SYEVIVFDTA PTGHTLRFLQ FPTVLEKALA KLSQLSSQFG PMLNSILGAR
210 220 230 240 250
GGLPGGQNID ELLQKMESLR ETISEVNTQF KNPDMTTFVC VCIAEFLSLY
260 270 280 290 300
ETERMIQELT SYGIDTHAIV VNQLLFPKEG SGCEQCNARR KMQKKYLEQI
310 320 330 340
EELYEDFNVV RMPLLVEEVR GKEKLEKFSE MLVHPYVPPQ
Length:340
Mass (Da):37,440
Last modified:April 17, 2007 - v2
Checksum:i9F6B277957FFE461
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000002 Genomic DNA. Translation: EAL92446.2.
RefSeqiXP_754484.2. XM_749391.2.

Genome annotation databases

EnsemblFungiiCADAFUAT00005643; CADAFUAP00005643; CADAFUAG00005643.
GeneIDi3512551.
KEGGiafm:AFUA_3G11350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000002 Genomic DNA. Translation: EAL92446.2.
RefSeqiXP_754484.2. XM_749391.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IBGX-ray3.20A/B/C/D/E/F3-340[»]
ProteinModelPortaliQ4WY07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48950N.

Proteomic databases

PRIDEiQ4WY07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00005643; CADAFUAP00005643; CADAFUAG00005643.
GeneIDi3512551.
KEGGiafm:AFUA_3G11350.

Organism-specific databases

EuPathDBiFungiDB:Afu3g11350.

Phylogenomic databases

HOGENOMiHOG000197637.
InParanoidiQ4WY07.
KOiK01551.
OMAiYEIDTHN.
OrthoDBiEOG7XDBRV.

Miscellaneous databases

EvolutionaryTraceiQ4WY07.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_03112. Asna1_Get3.
InterProiIPR025723. Anion-transp_ATPase-like_dom.
IPR016300. ATPase_ArsA/GET3.
IPR027542. ATPase_ArsA/GET3_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00345. GET3_arsA_TRC40. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  2. "Model for eukaryotic tail-anchored protein binding based on the structure of Get3."
    Suloway C.J.M., Chartron J.W., Zaslaver M., Clemons W.M. Jr.
    Proc. Natl. Acad. Sci. U.S.A. 106:14849-14854(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH ADP, SUBUNIT, MUTAGENESIS OF GLY-38.

Entry informationi

Entry nameiGET3_ASPFU
AccessioniPrimary (citable) accession number: Q4WY07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: April 17, 2007
Last modified: June 8, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.