Q4WXA1 (TRM10_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	trm10
ORF Names:	AFUA_3G08760

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060510

Sequences

	Sequence		Length	Mass (Da)
	Q4WXA1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: C3AC45ED7C2C21EF	FASTA	396	44,284
	10 20 30 40 50 60 MEDDDRPRKY PKLNHDEVQE GSEPVMTGAV GAVHDDDAES ADSDNRASPS NDRIDNDVKQ 70 80 90 100 110 120 ACDEEGQDAH GKDGPPTLSK NQLKKLKRKE HWEAMREQRK VKRKEKLVAK RERRRAALEQ 130 140 150 160 170 180 AKQEGAEATE ETRKAFESTQ KKFQRSTLLP VTLVLDCSYD DLMLDKERVS LGAQITRSYS 190 200 210 220 230 240 DNSRAPFRSH LVVSSFNKLL KERFDTVLGK THENWKGVRF LQEDFAEAAE MAKEWMQGPK 250 260 270 280 290 300 GGQLAGVFAD KADAKPEDGE IVYLSSDSPN ILTELKPYST YIIGGLVDKN RHKGICYKSA 310 320 330 340 350 360 VAKGIKTAKL PIGEYIQMAH RQVLATNHVV EIMIRWLELG DWGKAFIQVI PQRKGGKLKS 370 380 390 ADHESEDQTP RESVEAVEAE PDGEGAAAEA GEGGKE

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000002 Genomic DNA. Translation: EAL92702.1.
RefSeq	XP_754740.1. XM_749647.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4WXA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00004951; CADAFUAP00004951; CADAFUAG00004951.
GeneID	3512093.
GenomeReviews	Gene locus trm10 in contig CM000171_GR.
KEGG	afm:AFUA_3G08760.
Phylogenomic databases
eggNOG	fuNOG04600.
GeneTree	EFGT00050000003459.
HOGENOM	HBG717479.
OMA	RTAKLPI.
OrthoDB	EOG48D43Z.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM10_ASPFU

Accession

Primary (citable) accession number: Q4WXA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program