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Protein

Fucose-specific lectin

Gene

fleA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multispecific lectin that is able to recognize L-fucose in all possible linkages (PubMed:23695231, PubMed:27058347, PubMed:24340081, PubMed:25760594). These could be found not only in decomposed plant matter in soil, which is the natural environment for A.fumigatus, but also in various epitopes on human tissues (PubMed:25760594). Mediates binding of A.fumigatus conidia to airway mucinin a fucose dependent manner (PubMed:27058347). Stimulates IL-8 production by human bronchial cells in a dose-dependent manner, contributing to the inflammatory response observed upon the exposure of a patient to A.fumigatus, and thus might be an important virulence factor involved in an early stage of A.fumigatus infection (PubMed:24340081).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25Carbohydrate 1Combined sources1 Publication1
Binding sitei37Carbohydrate 1Combined sources1 Publication1
Binding sitei44Carbohydrate 6Combined sources1 Publication1
Binding sitei73Carbohydrate 2Combined sources1 Publication1
Binding sitei85Carbohydrate 2Combined sources1 Publication1
Binding sitei94Carbohydrate 1Combined sources1 Publication1
Binding sitei98Carbohydrate 2; via amide nitrogenCombined sources1 Publication1
Binding sitei126Carbohydrate 3Combined sources1 Publication1
Binding sitei138Carbohydrate 3Combined sources1 Publication1
Binding sitei146Carbohydrate 2Combined sources1 Publication1
Binding sitei150Carbohydrate 3Combined sources1 Publication1
Binding sitei177Carbohydrate 4Combined sources1 Publication1
Binding sitei198Carbohydrate 3Combined sources1 Publication1
Binding sitei230Carbohydrate 5Combined sources1 Publication1
Binding sitei242Carbohydrate 5Combined sources1 Publication1
Metal bindingi244ZincCombined sources1 Publication1
Metal bindingi246ZincCombined sources1 Publication1
Metal bindingi252Zinc; via tele nitrogenCombined sources1 Publication1
Binding sitei282Carbohydrate 6Combined sources1 Publication1
Binding sitei296Carbohydrate 6Combined sources1 Publication1

GO - Molecular functioni

Keywordsi

LigandLectin, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Fucose-specific lectin1 Publication
Alternative name(s):
Aspergillus fumigatus lectin1 Publication
Short name:
AFL1 Publication
Gene namesi
Name:fleA1 Publication
ORF Names:AFUA_5G14740
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:Afu5g14740.

Pathology & Biotechi

Biotechnological usei

Lectins have particular value as specific probes for investigating the distribution, structure and biological function of carbohydrate chains on the cell surface of animal, plant, and microorganism because of their specificity for defined carbohydrate structures. AFL is suitable for detecting core fucose on cell surface glycoproteins (PubMed:25760594).1 Publication

Disruption phenotypei

Increases lung infection and lung injury by A.fumigatus (PubMed:27058347).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004427451 – 315Fucose-specific lectinAdd BLAST315

Expressioni

Developmental stagei

Expressed in conidia (PubMed:27058347, PubMed:24340081).2 Publications

Interactioni

Subunit structurei

Homodimer (PubMed:25760594).1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AGIX-ray1.60A/B/C/D1-315[»]
4AGTX-ray2.00A/B1-315[»]
4AH4X-ray1.75A/B1-315[»]
4AHAX-ray2.20A/B1-315[»]
4C1YX-ray2.23A/B/C/D2-315[»]
4D4UX-ray1.99A/B1-315[»]
4D52X-ray1.76A/B/C/D1-315[»]
4UOUX-ray2.40A/B/C/D2-315[»]
ProteinModelPortaliQ4WW81.
SMRiQ4WW81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 5311 PublicationAdd BLAST52
Repeati54 – 10321 PublicationAdd BLAST50
Repeati104 – 15531 PublicationAdd BLAST52
Repeati156 – 20741 PublicationAdd BLAST52
Repeati208 – 26051 PublicationAdd BLAST53
Repeati261 – 31561 PublicationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 3156 X approximate tandem repeats1 PublicationAdd BLAST314

Domaini

AFL adopts the six-bladed beta-propeller fold and contains 6 binding sites per monomer, each located between two adjacent blades (PubMed:25760594). The 6 binding sites that are non-equivalent, and owing to minor differences in amino-acid composition they exhibit a marked difference in specific ligand recognition (PubMed:24340081, PubMed:25760594).2 Publications

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000217480.
InParanoidiQ4WW81.
OMAiYGSIRES.
OrthoDBiEOG092C34YM.

Family and domain databases

InterProiView protein in InterPro
IPR012475. Fungal_lectin.
PfamiView protein in Pfam
PF07938. Fungal_lectin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4WW81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPGAQQVL FRTGIAAVNL TNHLRVYFQD VYGSIRESLY EGSWANGTEK
60 70 80 90 100
NVIGNAKLGS PVAATSKELK HIRVYTLTEG NTLQEFAYDS GTGWYNGGLG
110 120 130 140 150
GAKFQVAPYS RIAAVFLAGT DALQLRIYAQ KPDNTIQEYM WNGDGWKEGT
160 170 180 190 200
NLGGALPGTG IGATSFRYTD YNGPSIRIWF QTDDLKLVQR AYDPHKGWYP
210 220 230 240 250
DLVTIFDRAP PRTAIAATSF GAGNSSIYMR IYFVNSDNTI WQVCWDHGKG
260 270 280 290 300
YHDKGTITPV IQGSEVAIIS WGSFANNGPD LRLYFQNGTY ISAVSEWVWN
310
RAHGSQLGRS ALPPA
Length:315
Mass (Da):34,661
Last modified:July 5, 2005 - v1
Checksum:i22E0C1479074D154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000003 Genomic DNA. Translation: EAL91145.1.
RefSeqiXP_753183.1. XM_748090.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00006809; CADAFUAP00006809; CADAFUAG00006809.
GeneIDi3511258.
KEGGiafm:AFUA_5G14740.

Similar proteinsi

Entry informationi

Entry nameiLECF_ASPFU
AccessioniPrimary (citable) accession number: Q4WW81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2017
Last sequence update: July 5, 2005
Last modified: December 20, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families