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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

dot1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei357 – 3571S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
Gene namesi
Name:dot1
ORF Names:AFUA_5G12110
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:Afu5g12110.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000270604Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ4WVH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 502322DOT1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni308 – 3114S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni331 – 34010S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni393 – 3942S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
Contains 1 DOT1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000176257.
InParanoidiQ4WVH4.
KOiK11427.
OMAiMGFFDHL.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WVH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFFDHLQKG GAFSLQAQKP QIRKVVQTRP PPPSRSSSHT PVRSLSQTSP
60 70 80 90 100
PGRVKRPRDS TSRSVSRDPD HRPSKRLSTP LRNRKRPTPE QRFSSDDDAS
110 120 130 140 150
DTDTSFELRK RARTEDSAEP DLARRIRSLK AFSEENVKPL PLVHAADITS
160 170 180 190 200
KQKAGNFRRA FGGADRPTEI LLQYPSASLK ERFNLVVPRD NDDFKPIDDI
210 220 230 240 250
VHVIDIVSEN YIPESEADFF NNESTGIKRR LRRALAHSSE TEFREAVASY
260 270 280 290 300
NREIERLRRS GAIAKHLDAT HRLNLPLVER ILTQIYARTV SPRVESLRRY
310 320 330 340 350
ENGTDNVYGE LLPRFISTIF KETRLKSGHV FVDLGSGVGN VVLQAALEIG
360 370 380 390 400
CESWGCEMMA NACDLAELQQ TEFRARCRLW GIAPGKTNLV RGDFLQEQSI
410 420 430 440 450
IDVLKRADVV LINNQAFTPQ LNNEIINHFL DMKEGCQIVS LKSFVPAGHK
460 470 480 490 500
IQSRNLYSPI NLLKVKQLNY WSNSVSWTDV GGTYFIATKD SSRLKAFADS

ME
Length:502
Mass (Da):56,798
Last modified:July 5, 2005 - v1
Checksum:i267DE547C9D276E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000003 Genomic DNA. Translation: EAL91402.1.
RefSeqiXP_753440.1. XM_748347.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00005981; CADAFUAP00005981; CADAFUAG00005981.
GeneIDi3511508.
KEGGiafm:AFUA_5G12110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000003 Genomic DNA. Translation: EAL91402.1.
RefSeqiXP_753440.1. XM_748347.1.

3D structure databases

ProteinModelPortaliQ4WVH4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00005981; CADAFUAP00005981; CADAFUAG00005981.
GeneIDi3511508.
KEGGiafm:AFUA_5G12110.

Organism-specific databases

EuPathDBiFungiDB:Afu5g12110.

Phylogenomic databases

HOGENOMiHOG000176257.
InParanoidiQ4WVH4.
KOiK11427.
OMAiMGFFDHL.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiDOT1_ASPFU
AccessioniPrimary (citable) accession number: Q4WVH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2005
Last modified: February 17, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.