Reviewed, UniProtKB/Swiss-Prot Q4WVG8 (DNLI4_ASPFU)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig4
ORF Names:	AFUA_5G12050

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	979 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 979	979	DNA ligase 4		PRO_0000278375
Regions
	Domain	721 – 814	94	BRCT 1
	Domain	867 – 965	99	BRCT 2
Sites
	Active site	322	1	N6-AMP-lysine intermediate By similarity
	Metal binding	389	1	Magnesium 1 Potential
	Metal binding	490	1	Magnesium 2 Potential
	Binding site	320	1	ATP By similarity
	Binding site	327	1	ATP By similarity
	Binding site	349	1	ATP By similarity
	Binding site	495	1	ATP By similarity
	Binding site	506	1	ATP By similarity
	Binding site	512	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4WVG8-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 2E5C1E83EB5F302A	FASTA	979	111,878
	10 20 30 40 50 60 MDSDEIMPDE EHPNVPVGDE ESDIDEKYPN RPRNHSPTLP FHELFQTLFN PLGEIKKKPA 70 80 90 100 110 120 GAVAARRKVG PHGQSAANLN PLERRRDVIE RFISRWRKEV GDDIYPAFRL ILPDKDRDRA 130 140 150 160 170 180 MYGIKEKAIG KMLVKIMKID KNSEDGFNLL NWKLPGQAAT SRMTGDFAGR CFDVISKRPM 190 200 210 220 230 240 RTDVGDMLIE EVNEKLDKLS AASKEEQQLP ILAEFYRRMN PEELMWLIRI ILRQMKVGAT 250 260 270 280 290 300 ERTFFDVWHP DAENLYSISS SLRRVCWELH DPNIRLDAED RGISLMQCFQ PQLAQFQMDS 310 320 330 340 350 360 LDRMVARMRP TEEDPVFWIE EKLDGERMQL HMASDDSVPG GRRFRFWSRK AKEYTYLYGN 370 380 390 400 410 420 GIYDENGSLT RYLKDAFADG VQSLILDGEM ITWDPEQDAP APFGTLKTAA LSEQRNPFST 430 440 450 460 470 480 TGARPLLRVF DILYLNGRDL TGYTLRDRRK ALQKAVRPVH RRFEIHPYEE ATTKDQVEAA 490 500 510 520 530 540 LRKVVAEASE GLVLKNPRSP YRLNERHDDW MKVKPEYMTE FGESLDLIVI GGYYGSGRRG 550 560 570 580 590 600 GNLSSFLCGL RVDDGHASQG ASASKCYSFC KVGGGFNAAD YANIRHHTDG KWMEWNPKKP 610 620 630 640 650 660 PTAYIELAGR DAQYERPDMW IKPEDSVVIC VKAASVSASD QFRLGLTLRF PRFKRLRMDK 670 680 690 700 710 720 DWKSALSVQE FLDLKSNVEQ EHREKELNVD NSRRKRVKRT AKKPLTVAGY DMDEDVKYAG 730 740 750 760 770 780 PSGHIFDGLN FYILTDSSAP IKKTKPELEQ LVKANGGMFF QTNNAAPHTI CVADRRTVKA 790 800 810 820 830 840 ASLQKRGDVD IIRPSWIIDC VKQNEIDAGL PDFLLPFEPR HMFFATPGKH DEVASNVDQF 850 860 870 880 890 900 GDSYARDTTS EELSDCPESI TKLIERLQES VNSGHEMPCG LLFKSLTILF PHRNDDVSES 910 920 930 940 950 960 EAKTSSHPSI THVVIDPESS SKEISSLRGK WSRKPGRKVP HIVTVDWVEE SWKSRTLLDE 970 ERFQPKRCIS GIAKPYWPG

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAHF01000003 Genomic DNA. Translation: EAL91408.1.
RefSeq	XP_753446.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3511447.
GenomeReviews	Gene locus lig4 in contig CM000173_GR.
KEGG	afm:AFUA_5G12050.
Phylogenomic databases
HOGENOM	Q4WVG8.
OMA	YCVIAGS.
Enzyme and pathway databases
BRENDA	6.5.1.1. 18841.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_ASPFU

Accession

Primary (citable) accession number: Q4WVG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)