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Protein

tRNA wybutosine-synthesizing protein 4

Gene

ppm2

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe).
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO2 = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionineBy similarity
Binding sitei95 – 951S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei122 – 1221S-adenosyl-L-methionineBy similarity
Binding sitei196 – 1961S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 4 (EC:2.1.1.290, EC:2.3.1.231)
Short name:
tRNA-yW synthesizing protein 4
Alternative name(s):
Leucine carboxyl methyltransferase 2
tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
Gene namesi
Name:ppm2
Synonyms:tyw4
ORF Names:AFUA_5G11670
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:Afu5g11670.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047tRNA wybutosine-synthesizing protein 4PRO_0000226137Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ4WVD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini814 – 1003190JmjCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1702S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000175968.
InParanoidiQ4WVD1.
KOiK15451.
OrthoDBiEOG7K0ZP5.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR011043. Gal_Oxase/kelch_b-propeller.
IPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 2 hits.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WVD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAAKKPAAA GVSTKAEREA DLVMETNNSS IVSKRSVELL YYPKPHFFRY
60 70 80 90 100
FVKRPPRRSP LINRGYWLRM HAMAESVRQF MKQPSDKPKF VLNLGCGFDP
110 120 130 140 150
LPFILLSTDK SLCSTTRFVD IDYEKLMVNK KTAIRRTDEI TRLLENVEFL
160 170 180 190 200
SDESPIQIRS EQYLAIGCDL KNLKKLDDVL KTELLPSDCS ILFLAEVSLT
210 220 230 240 250
YMDVKSANAV LAWASKLNND SQFCILEQFF PDGPNHPFAS TMMKHFNKLG
260 270 280 290 300
APLYSIHEYR SLSEQEQRFR NAGWAHAQAR SLWDLWSDNE FVGSSLRAWL
310 320 330 340 350
DTVEPFDEWE EFALFASHYF LLVASTKPQT MVQELQKTPA LTTEPDISSQ
360 370 380 390 400
YVLLAGNNPR GGQRRFGALI PDSENSMGHH SGLGRQTRDV STDLYSTCKG
410 420 430 440 450
MTTPQLPFPP REVSARMCHT VTSLRGGDCL LVGGRASPAN AFQDCWLRQG
460 470 480 490 500
KQWQSTKSLP APRFRHSAVK ITLETDSESV LVYGGKSSDG SIFDTWLLWQ
510 520 530 540 550
THSNGWQEVE IQGARPPARF GACLESINQT TGVLFGGIGS DGIIIEDFWI
560 570 580 590 600
WKIRHRSDGT VFLELTDHTE HLQQTPLSQY IYRFGSTVTR TSRGLVIVGG
610 620 630 640 650
IIPRQIVPYE CEIMLLDVGE LLEYVENESS WGHRILSAIG LGGILQGARP
660 670 680 690 700
LLVGHVACAI DPDQVLILGG GAVCFSFGTF WTEGGWVLKP AGSTAQNNWT
710 720 730 740 750
LVPEAMHTPE PVASPKTPQI STALKLSSIR RIRVDTSEQF QQILADGKPV
760 770 780 790 800
IIEGSDIGPC TELWTKEYLT DVVGSDRKVV VHESQSENMN FQAKNFSYVT
810 820 830 840 850
KAFGDFLDEV HAGGRQYLRS ISAELPSKLP ANLAADFPGL KDDFKLPQAL
860 870 880 890 900
SLVTENAHSS PLRISGPVTM WLHYDVSSNT KQEGWKLRVA DRYAQVMANV
910 920 930 940 950
LCQIRGERRL VLFPPADVQY LQVPPGASSS TIDIFQNIKD GSIVSIPHTS
960 970 980 990 1000
PQEAVLNSGD ILFIPPMWLH TASPTGGVSV AVNVFFRSLP KGYAAGRDVY
1010 1020 1030 1040
GNRDLQAYEK ARIDIQKMVR SFDGLPSDIS RFYLLRLAQE LKDNAGV
Length:1,047
Mass (Da):116,731
Last modified:March 7, 2006 - v2
Checksum:i0BFEB53A6487A2AA
GO

Sequence cautioni

The sequence EAL91445.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000003 Genomic DNA. Translation: EAL91445.1. Sequence problems.
RefSeqiXP_753483.1. XM_748390.1.

Genome annotation databases

GeneIDi3511201.
KEGGiafm:AFUA_5G11670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000003 Genomic DNA. Translation: EAL91445.1. Sequence problems.
RefSeqiXP_753483.1. XM_748390.1.

3D structure databases

ProteinModelPortaliQ4WVD1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3511201.
KEGGiafm:AFUA_5G11670.

Organism-specific databases

EuPathDBiFungiDB:Afu5g11670.

Phylogenomic databases

HOGENOMiHOG000175968.
InParanoidiQ4WVD1.
KOiK15451.
OrthoDBiEOG7K0ZP5.

Enzyme and pathway databases

UniPathwayiUPA00375.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR011043. Gal_Oxase/kelch_b-propeller.
IPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 2 hits.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiTYW4_ASPFU
AccessioniPrimary (citable) accession number: Q4WVD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 11, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.