Q4WUK5 (BTGC_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glucan endo-1,3-beta-glucosidase btgC
EC=3.2.1.39

Alternative name(s):
Endo-1,3-beta-glucanase btgC
Laminarinase btgC

Gene names

Name:	btgC
ORF Names:	AFUA_5G08780

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Acive on laminarin and lichenan By similarity.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Probable glucan endo-1,3-beta-glucosidase btgC		PRO_0000395124
Regions
	Topological domain	1 – 307	307	Cytoplasmic Potential
	Transmembrane	308 – 328	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	329 – 688	360	Extracellular Potential
	Compositional bias	254 – 336	83	Gly-rich
	Compositional bias	341 – 350	10	Poly-Ser
Sites
	Active site	590	1	Nucleophile By similarity
	Active site	658	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	431	1	N-linked (GlcNAc...) Potential
	Glycosylation	459	1	N-linked (GlcNAc...) Potential
	Glycosylation	609	1	N-linked (GlcNAc...) Potential
	Glycosylation	635	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4WUK5 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: BFE0AA05F1D71214	FASTA	688	72,696
	10 20 30 40 50 60 MSGPNRTYSF GEGDDSLAHP SSRTHAMHSQ YDDVSPISDG ARMNPMNGQG MDHGLASVLE 70 80 90 100 110 120 DGRQGWGRSP EPSPSLLTGS SATPGMDNLG PGAVGGGISG IALSVANSHD RLSGVEALMG 130 140 150 160 170 180 TDGQEANIPA ERGLSTTGSD NPYVPEPPEH RYSYGSNIAL GAAAAPAGQL TPGQSVSHLS 190 200 210 220 230 240 STNPSQRNLY DIPYQDVGGL NAGPYQRHSA YSSNDLPVDI NPDEIVDDGD DGFVPAPNSG 250 260 270 280 290 300 SGARKSQAIP AAAGGAAAGG VLGNLGGLFG GKSAADTSYG PVPGAGLEAG EKGRWVKPKP 310 320 330 340 350 360 GGGNKKRGWI VGAILAFIII GAIVGGAVGG TIGHRGNEEP SSASSASSSS TQTATEDTSV 370 380 390 400 410 420 NGDLDKNSAE IKALMNNKNL HKVFPGIDYT PWGVQYPLCL KYPPSQNNVT RDMAVLTQLT 430 440 450 460 470 480 NNVRLYGTDC NQTEMVLHAI DKLEIKDMKI WLGVWIDSNE TTSRRQIDQL YKIIDDAKDI 490 500 510 520 530 540 SIFNGAIVGN EALYRAGSDK TSAQTTLINY MQEVKDHFKK KNIDLPVATS DLGDNWDATL 550 560 570 580 590 600 VQAADVVMAN VHPFFGGIPV DQAAAWTWRF WQDHNVALTK GTNKKQIISE VGWPSGGGND 610 620 630 640 650 660 CGQGANCPND TAGAVAGVDE LNKFMEDWVC QALDNGTDYF WFEAFDEPWK IVYNTGKENW 670 680 EDKWGLMDSA RNLKPGLKIP DCGGKTAT

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000003 Genomic DNA. Translation: EAL91721.1.
RefSeq	XP_753759.1. XM_748666.1.
3D structure databases
ProteinModelPortal	Q4WUK5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00005902; CADAFUAP00005902; CADAFUAG00005902.
GeneID	3510833.
KEGG	afm:AFUA_5G08780.
Phylogenomic databases
eggNOG	COG5309.
HOGENOM	HOG000173877.
KO	K01238.
OMA	TEMVLHA.
OrthoDB	EOG4M0JC1.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BTGC_ASPFU

Accession

Primary (citable) accession number: Q4WUK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program