Q4WTN5 (BUR1_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase bur1
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	bur1
ORF Names:	AFUA_5G05510

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Serine/threonine-protein kinase bur1		PRO_0000085678
Regions
	Domain	25 – 326	302	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4WTN5 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: F2BBE96748382704	FASTA	580	65,107
	10 20 30 40 50 60 MAIASLERDD HGNPRFRGCT SIRDFEFLGK LGEGTFGEVY KARSKKDGSI VALKKILMHN 70 80 90 100 110 120 EKDGFPITAL REIKLLKMLS HRNILQLKEM AVERSKGDGR KKPSMYMVTP YMEHDLSGLL 130 140 150 160 170 180 ENPAVNFTEP QIKCYMLQLL EGLKYLHGNR ILHRDMKAAN LLISNNGVLQ IADFGLARPY 190 200 210 220 230 240 DEPPPEPGKG GGEAKRDYTT LVVTRWYRPP ELLLQLRRYT TAIDMWGVGC VFGEMFKGKP 250 260 270 280 290 300 ILAGSSDLNQ TQLIFNLVGT PTEENMPGWS SLPGCEGVKS FGYKPGSLRE VFKDQNPMAI 310 320 330 340 350 360 SLLEELLKLD WRKRINAIDA INHPYFSSPP FPARPGELPS FEDSHEFDRR RFRGQRGAIP 370 380 390 400 410 420 PAPAGGSVDQ QQTTEIAVFP VLHALAGSLH TGFKTILQSA LLKTGMLYHM DLQTRGSRFQ 430 440 450 460 470 480 ASLVQKIAAC LLNLLCLLIH HGAWVIQIGR HVREVNAIIS EDGPMGNWIH TSRLTVTLVD 490 500 510 520 530 540 ILEKGKAIVR VPLLIGVIWN SNTLMKGINR VILGTDVLQE REAGVLTFER LEQRIEHCTD 550 560 570 580 DDKLVLVSTD AERLRQMNPV TLLVLNDSKF ITHLLLSMTY

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000003 Genomic DNA. Translation: EAL92041.1.
RefSeq	XP_754079.1. XM_748986.1.
3D structure databases
ProteinModelPortal	Q4WTN5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00006445; CADAFUAP00006445; CADAFUAG00006445.
GeneID	3511249.
KEGG	afm:AFUA_5G05510.
Phylogenomic databases
eggNOG	COG0515.
KO	K15562.
OMA	KILMHNE.
OrthoDB	EOG41K2MW.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_ASPFU

Accession

Primary (citable) accession number: Q4WTN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program